ENSG00000155189

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000285518 ENSG00000155189 HEK293_OSMI2_6hA HEK293_TMG_6hB AGPAT5 protein_coding protein_coding 9.062154 2.618833 14.74378 0.5719479 0.4270659 2.48859 8.3504244 2.2581880 13.5139017 0.52678215 0.4273211 2.57589927 0.8721500 0.8538333 0.91646667 0.06263333 0.43851428 0.03810878 FALSE TRUE
ENST00000523586 ENSG00000155189 HEK293_OSMI2_6hA HEK293_TMG_6hB AGPAT5 protein_coding nonsense_mediated_decay 9.062154 2.618833 14.74378 0.5719479 0.4270659 2.48859 0.3275509 0.2941026 0.3001403 0.07630639 0.1677882 0.02836285 0.0705625 0.1130000 0.01983333 -0.09316667 0.03810878 0.03810878   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000155189 E001 8.2468086 0.0045759528 9.061084e-02 0.264900496 8 6708642 6708645 4 + 0.818 1.038 0.828
ENSG00000155189 E002 14.7077168 0.0021305742 2.701256e-01 0.510087064 8 6708646 6708665 20 + 1.064 1.181 0.422
ENSG00000155189 E003 24.2493270 0.0016015737 4.977409e-01 0.699299708 8 6708666 6708692 27 + 1.274 1.334 0.210
ENSG00000155189 E004 48.8298242 0.0049379878 4.735845e-01 0.682503519 8 6708693 6708859 167 + 1.571 1.618 0.161
ENSG00000155189 E005 31.2656523 0.0158358873 8.714181e-01 0.936711232 8 6708860 6708887 28 + 1.392 1.402 0.033
ENSG00000155189 E006 4.8125128 0.0843799559 2.776711e-05 0.000504223 8 6708888 6709162 275 + 0.438 1.156 2.935
ENSG00000155189 E007 3.2359583 0.0075690613 3.842145e-01 0.615766475 8 6709481 6709542 62 + 0.506 0.657 0.685
ENSG00000155189 E008 39.9582851 0.0009819660 7.688432e-01 0.877937251 8 6724870 6724939 70 + 1.499 1.478 -0.072
ENSG00000155189 E009 74.8808631 0.0033067555 9.933147e-02 0.280578635 8 6730711 6730826 116 + 1.779 1.679 -0.339
ENSG00000155189 E010 88.3522101 0.0006348930 9.659623e-01 0.984874849 8 6732561 6732650 90 + 1.836 1.834 -0.006
ENSG00000155189 E011 0.1779838 0.0361072834 1.000000e+00   8 6738376 6738550 175 + 0.060 0.000 -7.159
ENSG00000155189 E012 72.6639375 0.0005506805 5.166969e-01 0.712822500 8 6741661 6741751 91 + 1.743 1.777 0.114
ENSG00000155189 E013 0.8048655 0.0206148522 3.993462e-01   8 6745755 6746241 487 + 0.239 0.000 -9.631
ENSG00000155189 E014 51.8551566 0.0006914928 4.377688e-01 0.657042995 8 6747670 6747683 14 + 1.598 1.646 0.162
ENSG00000155189 E015 112.8234120 0.0022431207 2.567669e-01 0.495533083 8 6747684 6747828 145 + 1.929 1.979 0.169
ENSG00000155189 E016 104.3929031 0.0038098220 3.966748e-01 0.625722036 8 6755051 6755174 124 + 1.912 1.868 -0.148
ENSG00000155189 E017 790.6385289 0.0002816784 1.247271e-01 0.323317894 8 6757163 6761503 4341 + 2.784 2.765 -0.061