ENSG00000154822

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000432376 ENSG00000154822 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL2 protein_coding protein_coding 3.308868 2.131316 4.821351 0.3402693 0.6695633 1.173929 1.0379476 0.7421392 2.4493702 0.42488402 0.3022271 1.709217 0.3666250 0.38236667 0.5171000 0.13473333 8.929953e-01 1.563488e-15 FALSE TRUE
ENST00000615277 ENSG00000154822 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL2 protein_coding protein_coding 3.308868 2.131316 4.821351 0.3402693 0.6695633 1.173929 1.2478641 0.4424635 1.6207037 0.44246354 0.5039792 1.849621 0.2896792 0.17006667 0.3275667 0.15750000 4.443179e-01 1.563488e-15 FALSE TRUE
MSTRG.22538.2 ENSG00000154822 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL2 protein_coding   3.308868 2.131316 4.821351 0.3402693 0.6695633 1.173929 0.5707068 0.1700352 0.6466966 0.10756232 0.1489064 1.866948 0.1381125 0.06753333 0.1373667 0.06983333 6.602230e-01 1.563488e-15 FALSE TRUE
MSTRG.22538.4 ENSG00000154822 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL2 protein_coding   3.308868 2.131316 4.821351 0.3402693 0.6695633 1.173929 0.4002033 0.7333217 0.0000000 0.09308783 0.0000000 -6.215915 0.1885750 0.35053333 0.0000000 -0.35053333 1.563488e-15 1.563488e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000154822 E001 0.0000000       3 16802651 16803088 438 +      
ENSG00000154822 E002 3.2723577 0.4221582151 0.29479598 0.53532585 3 16884348 16884536 189 + 0.680 0.531 -0.659
ENSG00000154822 E003 2.2983758 0.3978433525 0.35117939 0.58832915 3 16884822 16884954 133 + 0.553 0.465 -0.423
ENSG00000154822 E004 1.1276969 0.2564121123 0.03739458   3 16884955 16885157 203 + 0.451 0.000 -10.980
ENSG00000154822 E005 8.2564776 0.2837046225 0.70464244 0.83850338 3 16885158 16885366 209 + 0.911 1.014 0.381
ENSG00000154822 E006 0.0000000       3 16933090 16933195 106 +      
ENSG00000154822 E007 0.0000000       3 16933196 16933291 96 +      
ENSG00000154822 E008 29.6525093 0.0016957655 0.25664441 0.49542368 3 17009674 17010086 413 + 1.515 1.434 -0.280
ENSG00000154822 E009 33.5801837 0.0106103008 0.01458306 0.07653378 3 17010087 17010493 407 + 1.611 1.412 -0.683
ENSG00000154822 E010 33.6271649 0.0013444137 0.41717761 0.64152886 3 17010494 17010904 411 + 1.561 1.505 -0.193
ENSG00000154822 E011 98.3488735 0.0010289331 0.69440244 0.83195531 3 17010905 17012041 1137 + 2.009 1.988 -0.068
ENSG00000154822 E012 22.0898494 0.0019155533 0.63018442 0.79050834 3 17012042 17012160 119 + 1.351 1.379 0.096
ENSG00000154822 E013 33.1111084 0.0013627029 0.20641999 0.43748027 3 17014708 17014911 204 + 1.505 1.575 0.238
ENSG00000154822 E014 24.1980954 0.0016129845 0.28025464 0.52039979 3 17042874 17042949 76 + 1.371 1.440 0.239
ENSG00000154822 E015 29.2190074 0.0016296273 0.96248576 0.98319829 3 17067956 17068065 110 + 1.484 1.482 -0.006
ENSG00000154822 E016 0.4764247 0.0242907082 0.96628307   3 17081188 17081546 359 + 0.181 0.147 -0.363
ENSG00000154822 E017 66.0770503 0.0007280587 0.00710585 0.04510828 3 17089733 17090604 872 + 1.783 1.884 0.341