ENSG00000154473

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368865 ENSG00000154473 HEK293_OSMI2_6hA HEK293_TMG_6hB BUB3 protein_coding protein_coding 199.8177 99.21882 263.6706 13.7427 4.944612 1.409961 12.18145 4.855298 17.37845 0.8019293 0.3813907 1.837529 0.05860417 0.04853333 0.0659000 0.01736667 0.15354465 0.01139475 FALSE TRUE
ENST00000407911 ENSG00000154473 HEK293_OSMI2_6hA HEK293_TMG_6hB BUB3 protein_coding protein_coding 199.8177 99.21882 263.6706 13.7427 4.944612 1.409961 86.96095 46.778492 105.38777 6.5642448 6.0668849 1.171619 0.45313750 0.47166667 0.3992667 -0.07240000 0.32523678 0.01139475 FALSE TRUE
ENST00000481952 ENSG00000154473 HEK293_OSMI2_6hA HEK293_TMG_6hB BUB3 protein_coding processed_transcript 199.8177 99.21882 263.6706 13.7427 4.944612 1.409961 16.85057 5.698498 34.70450 0.4553453 0.9078559 2.604355 0.07674167 0.05996667 0.1317000 0.07173333 0.01139475 0.01139475 FALSE FALSE
MSTRG.4762.2 ENSG00000154473 HEK293_OSMI2_6hA HEK293_TMG_6hB BUB3 protein_coding   199.8177 99.21882 263.6706 13.7427 4.944612 1.409961 79.53029 39.247203 102.59084 5.9753160 4.4939325 1.386013 0.38722500 0.39380000 0.3895000 -0.00430000 1.00000000 0.01139475 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000154473 E001 6.2440406 0.0042388021 6.145946e-01 7.799513e-01 10 123154267 123154323 57 + 0.852 0.796 -0.218
ENSG00000154473 E002 26.1320725 0.0376868509 3.460480e-01 5.836014e-01 10 123154324 123154401 78 + 1.429 1.333 -0.334
ENSG00000154473 E003 96.1909493 0.0077347982 4.017433e-01 6.298142e-01 10 123154402 123154414 13 + 1.966 1.935 -0.104
ENSG00000154473 E004 446.5645222 0.0001459806 1.073326e-01 2.945629e-01 10 123154415 123154485 71 + 2.607 2.649 0.140
ENSG00000154473 E005 24.5397390 0.0016086351 1.386772e-02 7.382380e-02 10 123154859 123154917 59 + 1.310 1.499 0.653
ENSG00000154473 E006 1175.0700684 0.0001252130 1.563156e-02 8.047409e-02 10 123154918 123155112 195 + 3.028 3.068 0.132
ENSG00000154473 E007 968.2467706 0.0007369896 2.284666e-01 4.634473e-01 10 123155658 123155727 70 + 2.946 2.977 0.104
ENSG00000154473 E008 3.2481727 0.0078460085 1.944250e-04 2.614238e-03 10 123157360 123157395 36 + 0.730 0.000 -12.604
ENSG00000154473 E009 1117.3585191 0.0009227430 2.222360e-01 4.561536e-01 10 123157729 123157880 152 + 3.009 3.040 0.103
ENSG00000154473 E010 1124.8326514 0.0005684730 2.464319e-01 4.840813e-01 10 123160407 123160565 159 + 3.013 3.042 0.094
ENSG00000154473 E011 0.1779838 0.0354605965 1.000000e+00   10 123161383 123161417 35 + 0.086 0.000 -8.283
ENSG00000154473 E012 457.3050226 0.0008502816 6.504463e-02 2.139930e-01 10 123162236 123162253 18 + 2.615 2.668 0.174
ENSG00000154473 E013 992.0270184 0.0016537249 4.549863e-02 1.687977e-01 10 123162254 123162413 160 + 2.950 3.003 0.178
ENSG00000154473 E014 1156.6670517 0.0015284476 5.135734e-02 1.829831e-01 10 123162612 123162828 217 + 3.016 3.067 0.168
ENSG00000154473 E015 218.8927229 0.0235232964 6.790585e-06 1.500387e-04 10 123162829 123163241 413 + 2.398 2.039 -1.199
ENSG00000154473 E016 358.4911952 0.0001682679 1.321268e-11 1.114110e-09 10 123163820 123163974 155 + 2.564 2.433 -0.435
ENSG00000154473 E017 1155.1899438 0.0001248315 3.160519e-52 1.192731e-48 10 123163975 123165046 1072 + 3.080 2.908 -0.574
ENSG00000154473 E018 929.7894108 0.0020463215 1.499093e-05 2.981059e-04 10 123165047 123170467 5421 + 2.899 3.013 0.381