ENSG00000154134

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000527196 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding retained_intron 10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.50946616 0.6347366 0.4279813 0.3032160 0.20717596 -0.5578407 0.052691667 0.044666667 0.09096667 0.04630000 7.814394e-01 5.544163e-06 FALSE TRUE
ENST00000527245 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding retained_intron 10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.70197029 1.0579221 0.4517754 0.4607412 0.06132647 -1.2095433 0.080087500 0.085700000 0.09923333 0.01353333 8.663848e-01 5.544163e-06 FALSE TRUE
ENST00000530647 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding retained_intron 10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.04259817 0.0000000 0.2904666 0.0000000 0.14993219 4.9091326 0.007916667 0.000000000 0.05890000 0.05890000 8.837148e-02 5.544163e-06   FALSE
ENST00000531119 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding retained_intron 10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.10173924 0.1030622 0.3096589 0.1030622 0.18724373 1.4994172 0.013016667 0.008933333 0.05943333 0.05050000 5.665118e-01 5.544163e-06   FALSE
ENST00000534598 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding retained_intron 10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.73287888 0.8732868 0.5304397 0.4970205 0.04579899 -0.7087483 0.086525000 0.069666667 0.12356667 0.05390000 6.691365e-01 5.544163e-06   FALSE
MSTRG.6509.11 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding   10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.60807691 0.5402521 0.4407970 0.3044916 0.23656655 -0.2876149 0.057525000 0.037533333 0.10786667 0.07033333 8.949907e-01 5.544163e-06 FALSE TRUE
MSTRG.6509.17 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding   10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.73982372 0.6937748 0.1638145 0.6937748 0.16381455 -2.0175649 0.071304167 0.060100000 0.04650000 -0.01360000 9.647535e-01 5.544163e-06 FALSE TRUE
MSTRG.6509.20 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding   10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 1.77976502 2.2256167 0.8659876 1.2300324 0.06995671 -1.3516906 0.165408333 0.155300000 0.19790000 0.04260000 9.022845e-01 5.544163e-06 FALSE TRUE
MSTRG.6509.21 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding   10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.95615155 0.7591881 0.0000000 0.2725098 0.00000000 -6.2652645 0.090904167 0.062333333 0.00000000 -0.06233333 1.083459e-02 5.544163e-06 FALSE TRUE
MSTRG.6509.22 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding   10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.99210540 2.0590788 0.0000000 0.5491927 0.00000000 -7.6928448 0.084008333 0.157033333 0.00000000 -0.15703333 5.544163e-06 5.544163e-06 FALSE TRUE
MSTRG.6509.25 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding   10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.80000214 1.7357734 0.3607588 0.4770750 0.18157837 -2.2353135 0.072579167 0.129233333 0.07163333 -0.05760000 7.807558e-01 5.544163e-06 FALSE TRUE
MSTRG.6509.6 ENSG00000154134 HEK293_OSMI2_6hA HEK293_TMG_6hB ROBO3 protein_coding   10.15154 13.22492 4.544105 1.5062 0.5893068 -1.53911 0.57984762 0.6180048 0.0000000 0.2391898 0.00000000 -5.9727036 0.050162500 0.051100000 0.00000000 -0.05110000 4.511616e-02 5.544163e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000154134 E001 0.4644705 0.023826039 0.622494832   11 124865432 124865737 306 + 0.000 0.171 9.375
ENSG00000154134 E002 0.0000000       11 124868468 124868801 334 +      
ENSG00000154134 E003 0.4341107 0.021768165 0.625398153   11 124868802 124869128 327 + 0.000 0.171 11.679
ENSG00000154134 E004 0.1308682 0.030851095 1.000000000   11 124869450 124869607 158 + 0.000 0.065 10.189
ENSG00000154134 E005 0.0000000       11 124869948 124870068 121 +      
ENSG00000154134 E006 0.3751086 0.027411536 1.000000000   11 124870165 124870303 139 + 0.000 0.121 11.139
ENSG00000154134 E007 0.3336024 0.027442404 1.000000000   11 124870601 124870728 128 + 0.000 0.121 11.136
ENSG00000154134 E008 0.2027342 0.054426639 1.000000000   11 124871014 124871138 125 + 0.000 0.065 10.137
ENSG00000154134 E009 0.3032425 0.024441170 1.000000000   11 124872381 124872552 172 + 0.000 0.121 11.142
ENSG00000154134 E010 0.1723744 0.033115352 1.000000000   11 124872884 124873089 206 + 0.000 0.065 10.186
ENSG00000154134 E011 0.5059767 0.021648130 0.618385782   11 124873310 124873391 82 + 0.000 0.171 11.682
ENSG00000154134 E012 0.1308682 0.030851095 1.000000000   11 124873697 124873862 166 + 0.000 0.065 10.189
ENSG00000154134 E013 0.6730019 0.019387492 0.179702766   11 124874070 124874236 167 + 0.361 0.121 -2.007
ENSG00000154134 E014 0.8453763 0.017276244 0.303619127   11 124874788 124874909 122 + 0.361 0.171 -1.422
ENSG00000154134 E015 0.9872031 0.015440520 0.862592025   11 124875111 124875336 226 + 0.217 0.256 0.315
ENSG00000154134 E016 0.6839606 0.019119330 0.793025911   11 124875564 124875657 94 + 0.217 0.171 -0.422
ENSG00000154134 E017 0.6486114 0.019310843 0.793756166   11 124875658 124875685 28 + 0.216 0.171 -0.419
ENSG00000154134 E018 0.7906259 0.092251322 1.000000000   11 124875954 124876120 167 + 0.216 0.216 0.000
ENSG00000154134 E019 0.1723744 0.033115352 1.000000000   11 124876121 124876125 5 + 0.000 0.065 10.186
ENSG00000154134 E020 3.7680227 0.009684761 0.048061604 0.17512693 11 124876126 124876194 69 + 0.834 0.532 -1.275
ENSG00000154134 E021 5.3556966 0.090198395 0.047346410 0.17338400 11 124876195 124876234 40 + 0.975 0.644 -1.312
ENSG00000154134 E022 6.1115233 0.050569181 0.043862319 0.16476433 11 124876235 124876274 40 + 1.004 0.694 -1.206
ENSG00000154134 E023 13.1736970 0.041420393 0.264866754 0.50418086 11 124876275 124876460 186 + 1.183 1.028 -0.557
ENSG00000154134 E024 0.3503582 0.027814917 0.308686160   11 124876466 124876469 4 + 0.217 0.065 -2.007
ENSG00000154134 E025 0.4812263 0.021768165 0.558092475   11 124876470 124876485 16 + 0.217 0.121 -1.009
ENSG00000154134 E026 1.9095049 0.010799381 0.275733851 0.51585200 11 124876486 124876697 212 + 0.555 0.359 -1.006
ENSG00000154134 E027 1.2912534 0.012717306 0.251020712   11 124876698 124876727 30 + 0.468 0.256 -1.270
ENSG00000154134 E028 1.1250361 0.015378759 0.171159482   11 124876728 124876738 11 + 0.468 0.216 -1.589
ENSG00000154134 E029 2.1183962 0.053577830 0.397232340 0.62611313 11 124876739 124876813 75 + 0.553 0.391 -0.819
ENSG00000154134 E030 1.4807435 0.211834509 0.888132916 0.94565018 11 124876814 124876817 4 + 0.357 0.332 -0.152
ENSG00000154134 E031 1.4807435 0.211834509 0.888132916 0.94565018 11 124876818 124876821 4 + 0.357 0.332 -0.152
ENSG00000154134 E032 1.6531178 0.279066312 0.974193067 0.98899689 11 124876822 124876825 4 + 0.356 0.366 0.054
ENSG00000154134 E033 2.0872285 0.141493950 0.743754227 0.86283823 11 124876826 124876830 5 + 0.358 0.444 0.473
ENSG00000154134 E034 2.2899627 0.081719495 0.621455881 0.78484050 11 124876831 124876837 7 + 0.359 0.469 0.598
ENSG00000154134 E035 2.4623371 0.121978565 0.574980891 0.75365658 11 124876838 124876840 3 + 0.358 0.494 0.723
ENSG00000154134 E036 2.6403209 0.081203203 0.930819237 0.96740829 11 124876841 124876841 1 + 0.468 0.493 0.126
ENSG00000154134 E037 3.4613067 0.093911650 0.931754461 0.96799521 11 124876842 124876845 4 + 0.553 0.577 0.108
ENSG00000154134 E038 16.1234393 0.002120669 0.662131768 0.81116985 11 124876846 124876874 29 + 1.102 1.152 0.180
ENSG00000154134 E039 27.0070283 0.001371210 0.901359397 0.95255774 11 124876875 124876932 58 + 1.361 1.356 -0.019
ENSG00000154134 E040 13.4587496 0.065776941 0.355611982 0.59221625 11 124876933 124877160 228 + 1.168 1.036 -0.476
ENSG00000154134 E041 17.0601555 0.002260010 0.591257423 0.76456159 11 124877161 124877184 24 + 1.201 1.152 -0.174
ENSG00000154134 E042 7.1001671 0.148828014 0.723198761 0.85007431 11 124877185 124877266 82 + 0.881 0.798 -0.322
ENSG00000154134 E043 20.4397369 0.001980346 0.045038461 0.16771333 11 124877267 124877284 18 + 1.374 1.207 -0.584
ENSG00000154134 E044 23.8284811 0.001442402 0.082885550 0.25050963 11 124877285 124877309 25 + 1.408 1.272 -0.474
ENSG00000154134 E045 6.3052193 0.045623183 0.591474513 0.76469675 11 124877310 124877387 78 + 0.689 0.792 0.418
ENSG00000154134 E046 8.4642566 0.179052744 0.728037306 0.85305808 11 124877388 124877518 131 + 0.874 0.889 0.054
ENSG00000154134 E047 39.0180676 0.006524609 0.674853367 0.81945045 11 124877519 124877562 44 + 1.530 1.500 -0.105
ENSG00000154134 E048 57.9084657 0.005564741 0.026883985 0.11795303 11 124877563 124877658 96 + 1.777 1.640 -0.464
ENSG00000154134 E049 22.7608242 0.010140828 0.244602728 0.48177554 11 124877659 124877752 94 + 1.363 1.253 -0.382
ENSG00000154134 E050 10.1955719 0.252416211 0.149066727 0.36091528 11 124877753 124877936 184 + 1.147 0.896 -0.923
ENSG00000154134 E051 17.3350529 0.073883424 0.162506672 0.38050643 11 124877937 124878131 195 + 1.314 1.130 -0.653
ENSG00000154134 E052 12.4652571 0.335902829 0.301254784 0.54171952 11 124878132 124878296 165 + 1.123 1.014 -0.393
ENSG00000154134 E053 5.5014349 0.206760593 0.358746149 0.59478345 11 124878297 124878297 1 + 0.831 0.703 -0.515
ENSG00000154134 E054 30.0127275 0.001863366 0.233205659 0.46885466 11 124878298 124878364 67 + 1.310 1.412 0.354
ENSG00000154134 E055 28.5451667 0.005215769 0.316741538 0.55671126 11 124878365 124878436 72 + 1.296 1.387 0.316
ENSG00000154134 E056 2.5432247 0.563085260 0.073612870 0.23198409 11 124878437 124878583 147 + 0.801 0.337 -2.184
ENSG00000154134 E057 82.4455573 0.001963310 0.145597580 0.35578252 11 124878584 124878796 213 + 1.763 1.842 0.267
ENSG00000154134 E058 19.6451699 0.099266221 0.427750178 0.64930074 11 124878797 124879189 393 + 1.285 1.200 -0.298
ENSG00000154134 E059 68.7134424 0.009492629 0.002963872 0.02318442 11 124879190 124879341 152 + 1.563 1.783 0.748
ENSG00000154134 E060 60.4472964 0.007725023 0.037657965 0.14873388 11 124879465 124879575 111 + 1.570 1.721 0.512
ENSG00000154134 E061 67.4981117 0.022236926 0.347941575 0.58537643 11 124879787 124879948 162 + 1.672 1.753 0.273
ENSG00000154134 E062 83.9655170 0.017560942 0.211439793 0.44348117 11 124880418 124880608 191 + 1.754 1.850 0.325
ENSG00000154134 E063 53.5796343 0.014388072 0.549479263 0.73592698 11 124881239 124881471 233 + 1.600 1.655 0.188