ENSG00000152527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000282406 ENSG00000152527 HEK293_OSMI2_6hA HEK293_TMG_6hB PLEKHH2 protein_coding protein_coding 1.01986 1.092385 1.260537 0.2441146 0.1800734 0.2048105 0.42059317 0.34960089 0.52641261 0.05220536 0.01670671 0.5769466 0.43402917 0.33470000 0.43093333 0.09623333 0.7795461386 0.0005052431 FALSE TRUE
ENST00000405000 ENSG00000152527 HEK293_OSMI2_6hA HEK293_TMG_6hB PLEKHH2 protein_coding retained_intron 1.01986 1.092385 1.260537 0.2441146 0.1800734 0.2048105 0.12351520 0.08228550 0.21412061 0.02385027 0.01247151 1.2800994 0.11565000 0.07680000 0.17773333 0.10093333 0.4149788629 0.0005052431 TRUE TRUE
ENST00000405223 ENSG00000152527 HEK293_OSMI2_6hA HEK293_TMG_6hB PLEKHH2 protein_coding retained_intron 1.01986 1.092385 1.260537 0.2441146 0.1800734 0.2048105 0.05060176 0.00000000 0.20647590 0.00000000 0.05938346 4.4361345 0.04428333 0.00000000 0.18256667 0.18256667 0.0244115935 0.0005052431 FALSE TRUE
ENST00000480103 ENSG00000152527 HEK293_OSMI2_6hA HEK293_TMG_6hB PLEKHH2 protein_coding retained_intron 1.01986 1.092385 1.260537 0.2441146 0.1800734 0.2048105 0.04431383 0.07862879 0.03000281 0.07862879 0.03000281 -1.1476743 0.03407083 0.07810000 0.01883333 -0.05926667 0.9750234027 0.0005052431   FALSE
ENST00000490038 ENSG00000152527 HEK293_OSMI2_6hA HEK293_TMG_6hB PLEKHH2 protein_coding nonsense_mediated_decay 1.01986 1.092385 1.260537 0.2441146 0.1800734 0.2048105 0.15956460 0.29034052 0.00000000 0.02674896 0.00000000 -4.9085272 0.20578333 0.30353333 0.00000000 -0.30353333 0.0005052431 0.0005052431 FALSE TRUE
ENST00000491692 ENSG00000152527 HEK293_OSMI2_6hA HEK293_TMG_6hB PLEKHH2 protein_coding nonsense_mediated_decay 1.01986 1.092385 1.260537 0.2441146 0.1800734 0.2048105 0.02279772 0.05018654 0.00000000 0.02509755 0.00000000 -2.5894409 0.02098333 0.05126667 0.00000000 -0.05126667 0.4302474903 0.0005052431 FALSE FALSE
ENST00000493408 ENSG00000152527 HEK293_OSMI2_6hA HEK293_TMG_6hB PLEKHH2 protein_coding retained_intron 1.01986 1.092385 1.260537 0.2441146 0.1800734 0.2048105 0.19847370 0.24134246 0.28352501 0.24134246 0.19912745 0.2238291 0.14519583 0.15556667 0.18993333 0.03436667 0.8582049530 0.0005052431 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000152527 E001 0.6536007 0.019387492 0.20862974   2 43637260 43637299 40 + 0.099 0.345 2.241
ENSG00000152527 E002 0.6536007 0.019387492 0.20862974   2 43637300 43637307 8 + 0.099 0.345 2.241
ENSG00000152527 E003 1.7654652 0.012198908 0.47060411 0.68051262 2 43637308 43637338 31 + 0.359 0.535 0.921
ENSG00000152527 E004 2.1048648 0.010042211 0.85067877 0.92541587 2 43637339 43637379 41 + 0.447 0.535 0.435
ENSG00000152527 E005 3.1989437 0.044463986 0.27889205 0.51895293 2 43644671 43644796 126 + 0.661 0.537 -0.551
ENSG00000152527 E006 0.3453689 0.027381956 0.84331690   2 43658666 43658867 202 + 0.099 0.148 0.662
ENSG00000152527 E007 3.8511960 0.070630380 0.15642314 0.37151057 2 43678863 43678925 63 + 0.746 0.540 -0.893
ENSG00000152527 E008 0.0000000       2 43679517 43679638 122 +      
ENSG00000152527 E009 0.0000000       2 43692230 43692513 284 +      
ENSG00000152527 E010 5.2086040 0.070573482 0.16021055 0.37712157 2 43692514 43692663 150 + 0.850 0.670 -0.725
ENSG00000152527 E011 4.1267849 0.010776179 0.25712896 0.49589187 2 43694431 43694514 84 + 0.730 0.628 -0.427
ENSG00000152527 E012 4.9275186 0.005727996 0.13637863 0.34196951 2 43695143 43695224 82 + 0.806 0.667 -0.565
ENSG00000152527 E013 2.2853218 0.012460356 0.09016118 0.26408600 2 43697171 43697173 3 + 0.583 0.345 -1.221
ENSG00000152527 E014 6.9187686 0.038594754 0.10381638 0.28855953 2 43697174 43697356 183 + 0.945 0.767 -0.686
ENSG00000152527 E015 22.4714616 0.003614660 0.01959945 0.09454982 2 43699647 43700608 962 + 1.396 1.301 -0.331
ENSG00000152527 E016 3.0933236 0.007635197 0.12646406 0.32618529 2 43703981 43704056 76 + 0.663 0.481 -0.829
ENSG00000152527 E017 2.7948828 0.012416117 0.09484990 0.27269746 2 43706322 43706416 95 + 0.638 0.418 -1.047
ENSG00000152527 E018 4.2933824 0.025122230 0.31834017 0.55822689 2 43707401 43707545 145 + 0.751 0.665 -0.358
ENSG00000152527 E019 4.5415670 0.016960643 0.43949706 0.65828902 2 43709990 43710126 137 + 0.751 0.703 -0.193
ENSG00000152527 E020 3.4759943 0.007964840 0.95180329 0.97786463 2 43710220 43710330 111 + 0.611 0.668 0.243
ENSG00000152527 E021 2.6358676 0.015353788 0.04112539 0.15767421 2 43710489 43710575 87 + 0.638 0.345 -1.462
ENSG00000152527 E022 7.8820791 0.005217923 0.15795340 0.37366784 2 43710576 43712224 1649 + 0.965 0.875 -0.341
ENSG00000152527 E023 7.5039995 0.023567469 0.77297250 0.88048259 2 43712225 43712383 159 + 0.902 0.939 0.140
ENSG00000152527 E024 4.7807657 0.006604650 0.45180743 0.66696388 2 43720669 43720749 81 + 0.770 0.736 -0.135
ENSG00000152527 E025 8.3317330 0.004438777 0.11565985 0.30860861 2 43726272 43726451 180 + 1.000 0.897 -0.387
ENSG00000152527 E026 7.0014033 0.004809524 0.40472403 0.63209027 2 43729637 43729745 109 + 0.900 0.874 -0.098
ENSG00000152527 E027 3.2897107 0.007587812 0.94971303 0.97692281 2 43731490 43731540 51 + 0.611 0.667 0.242
ENSG00000152527 E028 3.5680152 0.007467169 0.68745172 0.82744709 2 43731541 43731602 62 + 0.611 0.736 0.530
ENSG00000152527 E029 6.3055588 0.004610128 0.44094851 0.65942648 2 43738341 43738520 180 + 0.870 0.850 -0.081
ENSG00000152527 E030 0.4231520 0.088876536 0.45161257   2 43740816 43740945 130 + 0.099 0.255 1.639
ENSG00000152527 E031 3.8607470 0.007923349 0.24423986 0.48135672 2 43740946 43740984 39 + 0.730 0.627 -0.435
ENSG00000152527 E032 3.6361248 0.006747417 0.99152696 0.99753097 2 43740985 43741043 59 + 0.638 0.703 0.277
ENSG00000152527 E033 0.6412370 0.022410196 0.06898155   2 43741044 43741126 83 + 0.307 0.000 -9.810
ENSG00000152527 E034 0.9770522 0.016271537 0.61832076   2 43741127 43741365 239 + 0.307 0.258 -0.343
ENSG00000152527 E035 6.7685311 0.005290477 0.41676828 0.64120453 2 43742741 43742918 178 + 0.806 0.978 0.654
ENSG00000152527 E036 7.6565967 0.050095074 0.79819135 0.89528873 2 43743834 43743989 156 + 0.903 0.959 0.211
ENSG00000152527 E037 1.1793539 0.388977579 0.88552480   2 43743990 43744134 145 + 0.310 0.351 0.253
ENSG00000152527 E038 4.8636625 0.007716536 0.02399433 0.10907114 2 43745866 43745898 33 + 0.840 0.584 -1.061
ENSG00000152527 E039 5.1694778 0.026297633 0.34468359 0.58221968 2 43745899 43745963 65 + 0.806 0.737 -0.278
ENSG00000152527 E040 7.1290968 0.004738744 0.15126460 0.36417546 2 43753619 43753760 142 + 0.788 1.030 0.918
ENSG00000152527 E041 6.2290225 0.005472235 0.08057816 0.24599608 2 43757119 43757264 146 + 0.708 0.997 1.119
ENSG00000152527 E042 6.1548125 0.009985307 0.08317255 0.25114050 2 43758900 43759029 130 + 0.708 0.997 1.121
ENSG00000152527 E043 4.2005358 0.006147329 0.15378421 0.36772882 2 43762304 43762390 87 + 0.583 0.850 1.106
ENSG00000152527 E044 5.7519370 0.058532568 0.33329123 0.57189569 2 43764228 43764365 138 + 0.729 0.952 0.870
ENSG00000152527 E045 39.3997596 0.702257086 0.11211247 0.30261335 2 43765413 43767987 2575 + 1.312 1.838 1.798