ENSG00000152219

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000282032 ENSG00000152219 HEK293_OSMI2_6hA HEK293_TMG_6hB ARL14EP protein_coding protein_coding 18.4153 5.445202 31.50053 0.3325603 1.805232 2.53013 9.042626 2.8438552 15.494180 0.6165297 0.5998341 2.4416732 0.4631208 0.5130333 0.4932000 -0.019833333 0.983584119 0.002580657 FALSE TRUE
ENST00000532047 ENSG00000152219 HEK293_OSMI2_6hA HEK293_TMG_6hB ARL14EP protein_coding retained_intron 18.4153 5.445202 31.50053 0.3325603 1.805232 2.53013 5.283982 1.0350199 8.617363 0.5309904 0.3408058 3.0453893 0.2723000 0.1934667 0.2741333 0.080666667 0.859378888 0.002580657 FALSE FALSE
ENST00000533457 ENSG00000152219 HEK293_OSMI2_6hA HEK293_TMG_6hB ARL14EP protein_coding processed_transcript 18.4153 5.445202 31.50053 0.3325603 1.805232 2.53013 2.367005 0.7476356 4.835269 0.3752555 0.5009395 2.6770008 0.1372000 0.1445667 0.1529000 0.008333333 0.929735834 0.002580657   FALSE
MSTRG.5328.1 ENSG00000152219 HEK293_OSMI2_6hA HEK293_TMG_6hB ARL14EP protein_coding   18.4153 5.445202 31.50053 0.3325603 1.805232 2.53013 1.091285 0.7364199 1.208228 0.1222923 0.1438210 0.7067248 0.1017708 0.1334000 0.0391000 -0.094300000 0.002580657 0.002580657 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000152219 E001 0.1426347 0.0329642624 1.00000000   11 30323093 30323103 11 + 0.066 0.000 -7.703
ENSG00000152219 E002 0.2027342 0.0341968194 0.10968059   11 30323104 30323109 6 + 0.000 0.212 11.285
ENSG00000152219 E003 38.3595804 0.0128402487 0.27933155 0.51944595 11 30323110 30323132 23 + 1.491 1.566 0.254
ENSG00000152219 E004 74.4365722 0.0012613951 0.02418420 0.10965486 11 30323133 30323202 70 + 1.766 1.865 0.334
ENSG00000152219 E005 2.2583424 0.0107650453 0.93273434 0.96851262 11 30327006 30327058 53 + 0.450 0.460 0.051
ENSG00000152219 E006 25.5232694 0.0329071500 0.07686595 0.23853967 11 30328036 30330885 2850 + 1.377 1.138 -0.842
ENSG00000152219 E007 247.8515383 0.0003694876 0.59557853 0.76744947 11 30330886 30331374 489 + 2.312 2.291 -0.070
ENSG00000152219 E008 4.0800717 0.0069335882 0.01413784 0.07490737 11 30331375 30331402 28 + 0.711 0.212 -2.719
ENSG00000152219 E009 12.1326470 0.0031967655 0.60832400 0.77581616 11 30331644 30331800 157 + 1.058 0.991 -0.246
ENSG00000152219 E010 126.8151606 0.0005150581 0.02735046 0.11935290 11 30332866 30332993 128 + 2.002 2.072 0.233
ENSG00000152219 E011 341.3638897 0.0036093965 0.15027172 0.36268760 11 30336567 30338223 1657 + 2.458 2.412 -0.152