ENSG00000149658

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370339 ENSG00000149658 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF1 protein_coding protein_coding 37.53902 31.16829 31.98017 2.319827 1.512188 0.03708693 32.7597481 27.810639 27.090386 2.2730152 1.8906953 -0.03784213 0.874687500 0.8914 0.84523333 -0.046166667 5.275857e-01 4.050699e-08 FALSE TRUE
MSTRG.20970.2 ENSG00000149658 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF1 protein_coding   37.53902 31.16829 31.98017 2.319827 1.512188 0.03708693 2.7352276 2.013541 1.959879 0.1529957 0.2579320 -0.03877506 0.071500000 0.0659 0.06123333 -0.004666667 9.671438e-01 4.050699e-08 TRUE TRUE
MSTRG.20970.5 ENSG00000149658 HEK293_OSMI2_6hA HEK293_TMG_6hB YTHDF1 protein_coding   37.53902 31.16829 31.98017 2.319827 1.512188 0.03708693 0.2285413 0.000000 1.676498 0.0000000 0.5083325 7.39788698 0.007183333 0.0000 0.05423333 0.054233333 4.050699e-08 4.050699e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000149658 E001 17.42783 0.13402357 0.571718310 0.75146025 20 63195429 63195455 27 - 1.174 1.342 0.589
ENSG00000149658 E002 182.85323 1.11181338 0.422275412 0.64536288 20 63195456 63195586 131 - 2.093 2.382 0.967
ENSG00000149658 E003 337.39841 1.35782574 0.467630588 0.67846221 20 63195587 63195770 184 - 2.365 2.643 0.929
ENSG00000149658 E004 522.58786 1.47924153 0.398990014 0.62737434 20 63195771 63196366 596 - 2.497 2.859 1.204
ENSG00000149658 E005 122.13692 0.67122112 0.243913413 0.48093660 20 63196367 63196454 88 - 1.844 2.240 1.330
ENSG00000149658 E006 359.44945 1.36539915 0.441466504 0.65971935 20 63196455 63196734 280 - 2.374 2.680 1.017
ENSG00000149658 E007 1306.62568 0.13544141 0.117642833 0.31192374 20 63202287 63203799 1513 - 3.098 3.133 0.118
ENSG00000149658 E008 102.51254 0.01689358 0.019451627 0.09401832 20 63203800 63203807 8 - 2.012 2.017 0.017
ENSG00000149658 E009 153.77428 0.01783495 0.005220299 0.03578529 20 63213864 63213943 80 - 2.199 2.180 -0.066
ENSG00000149658 E010 78.45887 0.02583025 0.003835180 0.02821687 20 63215577 63215601 25 - 1.937 1.859 -0.264
ENSG00000149658 E011 30.42397 0.07057770 0.230672387 0.46591273 20 63215602 63215865 264 - 1.495 1.500 0.019
ENSG00000149658 E012 55.30980 0.02765629 0.060839512 0.20468221 20 63215866 63216139 274 - 1.752 1.749 -0.012