ENSG00000149547

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000278903 ENSG00000149547 HEK293_OSMI2_6hA HEK293_TMG_6hB EI24 protein_coding protein_coding 253.5699 276.8262 201.1653 32.53558 7.985152 -0.4605797 191.263383 202.448328 160.477270 27.269348 9.3150635 -0.3351661 0.74991250 0.72796667 0.79663333 0.06866667 0.40282942 0.01350192 FALSE  
ENST00000527842 ENSG00000149547 HEK293_OSMI2_6hA HEK293_TMG_6hB EI24 protein_coding protein_coding 253.5699 276.8262 201.1653 32.53558 7.985152 -0.4605797 9.907428 9.210679 16.804526 2.707265 0.2857621 0.8667632 0.04569167 0.03266667 0.08386667 0.05120000 0.01350192 0.01350192 FALSE  
MSTRG.6527.4 ENSG00000149547 HEK293_OSMI2_6hA HEK293_TMG_6hB EI24 protein_coding   253.5699 276.8262 201.1653 32.53558 7.985152 -0.4605797 15.637380 20.099582 8.743837 1.592437 1.9031767 -1.1998956 0.06403750 0.07363333 0.04440000 -0.02923333 0.43125539 0.01350192 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000149547 E001 0.1723744 3.043373e-02 7.886038e-01   11 125569280 125569332 53 + 0.000 0.097 9.670
ENSG00000149547 E002 0.3751086 2.444117e-02 3.872038e-01   11 125569333 125569365 33 + 0.000 0.177 10.680
ENSG00000149547 E003 0.4644705 7.133210e-02 2.091130e-01   11 125569366 125569372 7 + 0.000 0.242 11.255
ENSG00000149547 E004 1.1540406 3.733144e-01 7.009691e-01   11 125569373 125569396 24 + 0.267 0.350 0.540
ENSG00000149547 E005 2.2793664 2.651801e-02 9.819918e-02 2.786239e-01 11 125569397 125569403 7 + 0.263 0.576 1.734
ENSG00000149547 E006 17.4148109 2.670314e-03 1.430536e-06 3.882658e-05 11 125569404 125569445 42 + 0.860 1.359 1.809
ENSG00000149547 E007 40.2275250 3.498207e-02 1.011664e-05 2.117636e-04 11 125569446 125569457 12 + 1.198 1.726 1.823
ENSG00000149547 E008 128.3437404 3.768162e-02 4.116665e-06 9.731410e-05 11 125569458 125569476 19 + 1.732 2.220 1.639
ENSG00000149547 E009 196.2896194 2.130402e-02 8.952443e-05 1.362182e-03 11 125569477 125569488 12 + 2.052 2.372 1.068
ENSG00000149547 E010 375.5229926 8.398251e-03 1.073841e-04 1.586990e-03 11 125569489 125569514 26 + 2.415 2.626 0.701
ENSG00000149547 E011 545.3279672 2.296194e-03 3.364715e-06 8.194551e-05 11 125569515 125569573 59 + 2.618 2.770 0.505
ENSG00000149547 E012 2.7852705 9.210063e-03 2.805543e-04 3.534989e-03 11 125569574 125569577 4 + 0.000 0.701 13.681
ENSG00000149547 E013 2.1243680 2.146460e-01 8.301175e-01 9.138393e-01 11 125569840 125569858 19 + 0.429 0.488 0.304
ENSG00000149547 E014 3.8054606 1.388384e-01 8.373731e-01 9.179703e-01 11 125569859 125569947 89 + 0.680 0.640 -0.168
ENSG00000149547 E015 60.3645313 7.393234e-04 9.105573e-01 9.573945e-01 11 125570104 125570190 87 + 1.760 1.767 0.022
ENSG00000149547 E016 1200.7630090 9.493861e-05 1.211856e-02 6.692530e-02 11 125572458 125572569 112 + 3.067 3.053 -0.047
ENSG00000149547 E017 10.3351441 3.481707e-02 8.201776e-01 9.081871e-01 11 125575251 125575262 12 + 1.023 1.058 0.130
ENSG00000149547 E018 1039.0608678 7.584518e-05 2.501596e-07 8.405787e-06 11 125575263 125575304 42 + 3.023 2.976 -0.156
ENSG00000149547 E019 851.7912266 1.030702e-04 3.828014e-07 1.222423e-05 11 125575305 125575308 4 + 2.940 2.888 -0.175
ENSG00000149547 E020 1804.9417007 8.621775e-05 8.363812e-09 3.998783e-07 11 125575309 125575408 100 + 3.259 3.220 -0.128
ENSG00000149547 E021 5.7280420 3.954627e-02 4.819678e-02 1.754606e-01 11 125575803 125575931 129 + 0.966 0.682 -1.114
ENSG00000149547 E022 1602.7075307 6.617319e-04 7.419697e-05 1.165404e-03 11 125576255 125576315 61 + 3.210 3.173 -0.123
ENSG00000149547 E023 1560.2787072 9.365881e-04 9.677648e-08 3.603349e-06 11 125577504 125577570 67 + 3.215 3.150 -0.216
ENSG00000149547 E024 9.7866308 2.604968e-02 1.399475e-01 3.474048e-01 11 125577571 125577735 165 + 1.128 0.953 -0.641
ENSG00000149547 E025 10.6423507 2.858281e-03 2.785970e-02 1.208794e-01 11 125577736 125577906 171 + 1.168 0.955 -0.774
ENSG00000149547 E026 1908.4346967 1.214743e-04 3.349841e-09 1.744968e-07 11 125578133 125578257 125 + 3.284 3.245 -0.132
ENSG00000149547 E027 1677.0455201 2.150952e-04 5.759921e-01 7.544151e-01 11 125578949 125579068 120 + 3.197 3.208 0.038
ENSG00000149547 E028 1672.1279772 1.185902e-04 4.682488e-01 6.788850e-01 11 125580093 125580204 112 + 3.188 3.211 0.075
ENSG00000149547 E029 8.0960073 9.082646e-03 9.230578e-01 9.638128e-01 11 125580780 125581210 431 + 0.929 0.932 0.015
ENSG00000149547 E030 1497.6513207 1.042260e-04 7.866175e-02 2.420996e-01 11 125581211 125581322 112 + 3.132 3.165 0.109
ENSG00000149547 E031 1372.2276046 1.926076e-04 1.110569e-03 1.075074e-02 11 125582346 125582420 75 + 3.081 3.135 0.179
ENSG00000149547 E032 6752.0428065 8.909084e-04 4.875967e-04 5.563112e-03 11 125583521 125584684 1164 + 3.773 3.836 0.207