ENSG00000149499

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000278845 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding protein_coding 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 0.79739814 1.2955040 0.3045109 0.68064358 0.1559534 -2.053425 0.058500000 0.07070000 0.04970000 -2.100000e-02 9.923266e-01 3.652618e-05 FALSE  
ENST00000394773 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding protein_coding 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 2.32447534 2.9454053 0.9174399 0.26692918 0.5282535 -1.672030 0.195033333 0.18006667 0.16480000 -1.526667e-02 9.137523e-01 3.652618e-05 FALSE  
ENST00000394776 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding protein_coding 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 0.62883571 0.8607274 0.0000000 0.09420215 0.0000000 -6.444149 0.049458333 0.05280000 0.00000000 -5.280000e-02 3.652618e-05 3.652618e-05 FALSE  
ENST00000439994 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding protein_coding 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 1.02318719 1.6520425 0.5691783 0.35433039 0.3642809 -1.520878 0.089366667 0.09633333 0.08766667 -8.666667e-03 8.948427e-01 3.652618e-05 FALSE  
ENST00000460939 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding retained_intron 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 1.61118755 2.1057389 0.7701400 0.55830945 0.1648816 -1.439357 0.134233333 0.12583333 0.12576667 -6.666667e-05 1.000000e+00 3.652618e-05 TRUE  
ENST00000494176 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding protein_coding 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 0.07561246 0.0000000 0.3101930 0.00000000 0.1653015 5.000870 0.010508333 0.00000000 0.05470000 5.470000e-02 1.430521e-01 3.652618e-05 FALSE  
ENST00000524518 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding retained_intron 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 0.80679497 1.1155404 0.9122660 0.53234368 0.2858842 -0.287363 0.077633333 0.06463333 0.15820000 9.356667e-02 5.809327e-01 3.652618e-05    
ENST00000529309 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding protein_coding 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 0.04412913 0.0000000 0.3530330 0.00000000 0.3530330 5.182029 0.006508333 0.00000000 0.05206667 5.206667e-02 7.576542e-01 3.652618e-05 FALSE  
ENST00000531557 ENSG00000149499 HEK293_OSMI2_6hA HEK293_TMG_6hB EML3 protein_coding protein_coding 12.1314 16.8572 6.017325 1.455722 0.3972181 -1.484631 2.06865642 2.9600140 0.5381548 1.48602745 0.2834198 -2.437814 0.149304167 0.18293333 0.08713333 -9.580000e-02 9.520963e-01 3.652618e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000149499 E001 39.592625 0.0009653424 5.007288e-02 0.1798533095 11 62602218 62602218 1 - 1.608 1.478 -0.442
ENSG00000149499 E002 39.706737 0.0009878694 7.581819e-02 0.2364113784 11 62602219 62602219 1 - 1.600 1.483 -0.403
ENSG00000149499 E003 42.355978 0.0010036954 8.350158e-02 0.2517753043 11 62602220 62602225 6 - 1.621 1.509 -0.383
ENSG00000149499 E004 84.528511 0.0005990234 3.029507e-04 0.0037599943 11 62602226 62602353 128 - 1.954 1.791 -0.547
ENSG00000149499 E005 49.656599 0.0200758449 4.348284e-01 0.6548648359 11 62602354 62602595 242 - 1.549 1.617 0.233
ENSG00000149499 E006 38.586922 0.0011438201 6.408635e-06 0.0001428634 11 62602596 62602678 83 - 1.171 1.551 1.323
ENSG00000149499 E007 4.492046 0.0057787796 2.195164e-01 0.4528449711 11 62602679 62602758 80 - 0.474 0.694 0.999
ENSG00000149499 E008 28.029998 0.0014014227 5.823269e-02 0.1987837062 11 62602759 62602779 21 - 1.225 1.390 0.576
ENSG00000149499 E009 48.133001 0.0018622364 8.927809e-03 0.0534966084 11 62602780 62602889 110 - 1.437 1.619 0.624
ENSG00000149499 E010 19.407750 0.0556659061 4.937021e-01 0.6964341989 11 62602890 62603115 226 - 1.275 1.190 -0.302
ENSG00000149499 E011 8.972526 0.0707290209 5.609163e-01 0.7439588570 11 62603116 62603148 33 - 0.978 0.882 -0.361
ENSG00000149499 E012 54.995198 0.0009850989 1.151356e-01 0.3076739281 11 62603149 62603247 99 - 1.571 1.667 0.328
ENSG00000149499 E013 59.712508 0.0008804177 5.846612e-01 0.7601027825 11 62603729 62603816 88 - 1.654 1.684 0.100
ENSG00000149499 E014 62.110580 0.0139395130 6.321097e-01 0.7917563069 11 62603944 62604041 98 - 1.669 1.699 0.103
ENSG00000149499 E015 8.892163 0.1525750901 3.799758e-01 0.6121564213 11 62604042 62604072 31 - 1.016 0.864 -0.568
ENSG00000149499 E016 10.857097 0.2307117161 3.296585e-01 0.5685922556 11 62604073 62604112 40 - 1.118 0.935 -0.670
ENSG00000149499 E017 59.417490 0.0017179042 7.672832e-01 0.8769746649 11 62604113 62604201 89 - 1.691 1.671 -0.068
ENSG00000149499 E018 12.496027 0.2302925044 1.073037e-01 0.2945215532 11 62604708 62605112 405 - 1.294 0.944 -1.261
ENSG00000149499 E019 55.967039 0.0007081621 6.534585e-01 0.8057417434 11 62605113 62605180 68 - 1.635 1.660 0.085
ENSG00000149499 E020 5.049213 0.1328644009 6.330533e-01 0.7923513073 11 62605181 62605229 49 - 0.751 0.681 -0.286
ENSG00000149499 E021 2.687077 0.1979323414 2.076326e-01 0.4389820523 11 62605596 62605641 46 - 0.701 0.415 -1.327
ENSG00000149499 E022 69.158282 0.0286276870 7.806069e-01 0.8850884875 11 62605642 62605773 132 - 1.733 1.745 0.040
ENSG00000149499 E023 2.827482 0.2802920777 3.007769e-01 0.5412442017 11 62605837 62605854 18 - 0.689 0.448 -1.105
ENSG00000149499 E024 48.368752 0.0183610646 7.436565e-01 0.8627602122 11 62605855 62605909 55 - 1.572 1.598 0.088
ENSG00000149499 E025 45.394317 0.0103054299 6.691948e-01 0.8157037972 11 62605910 62605980 71 - 1.539 1.574 0.120
ENSG00000149499 E026 4.250952 0.2239657132 5.892272e-01 0.7631480956 11 62605981 62606062 82 - 0.697 0.621 -0.325
ENSG00000149499 E027 65.668188 0.0159738749 1.456653e-01 0.3558967178 11 62606063 62606214 152 - 1.626 1.744 0.398
ENSG00000149499 E028 62.159330 0.0009646362 1.175531e-01 0.3117544572 11 62606958 62607099 142 - 1.628 1.717 0.303
ENSG00000149499 E029 4.412295 0.4481336865 6.333240e-01 0.7925362993 11 62607100 62607392 293 - 0.631 0.655 0.104
ENSG00000149499 E030 50.702164 0.0009021715 1.908680e-01 0.4179869218 11 62607666 62607821 156 - 1.679 1.600 -0.267
ENSG00000149499 E031 2.271487 0.6216553270 6.060318e-01 0.7742306707 11 62607866 62608200 335 - 0.483 0.427 -0.287
ENSG00000149499 E032 49.365191 0.0011216579 5.633559e-01 0.7456374048 11 62608201 62608296 96 - 1.635 1.598 -0.124
ENSG00000149499 E033 61.084951 0.0007243055 8.018762e-01 0.8974425034 11 62608542 62608652 111 - 1.708 1.693 -0.051
ENSG00000149499 E034 49.502757 0.0008808289 8.785657e-01 0.9405636305 11 62608736 62608805 70 - 1.600 1.609 0.029
ENSG00000149499 E035 64.962921 0.0023794903 8.023986e-01 0.8977254458 11 62608962 62609132 171 - 1.730 1.714 -0.054
ENSG00000149499 E036 48.676460 0.0055525469 6.320722e-01 0.7917342346 11 62609354 62609477 124 - 1.571 1.606 0.118
ENSG00000149499 E037 34.730888 0.0014165708 9.485138e-01 0.9765114128 11 62609629 62609696 68 - 1.457 1.450 -0.023
ENSG00000149499 E038 33.519719 0.0014116323 7.563590e-01 0.8704505146 11 62610879 62610954 76 - 1.457 1.433 -0.085
ENSG00000149499 E039 20.831468 0.0018638316 4.734960e-01 0.6824454999 11 62610955 62610975 21 - 1.289 1.222 -0.236
ENSG00000149499 E040 17.569990 0.0021673729 9.615255e-01 0.9827885731 11 62610976 62610976 1 - 1.171 1.164 -0.024
ENSG00000149499 E041 20.867297 0.0016823531 8.485821e-01 0.9241236241 11 62610977 62610992 16 - 1.225 1.242 0.057
ENSG00000149499 E042 0.000000       11 62610993 62611007 15 -      
ENSG00000149499 E043 20.862133 0.0019818085 8.390639e-01 0.9188599450 11 62611087 62611111 25 - 1.258 1.238 -0.072
ENSG00000149499 E044 43.195875 0.0011385598 5.983867e-01 0.7691507387 11 62611112 62611344 233 - 1.578 1.543 -0.120
ENSG00000149499 E045 6.050471 0.0206621130 5.733256e-01 0.7525336089 11 62611345 62611347 3 - 0.840 0.756 -0.332
ENSG00000149499 E046 26.653461 0.0014052278 9.034023e-01 0.9535968909 11 62611425 62611596 172 - 1.357 1.345 -0.038
ENSG00000149499 E047 7.794254 0.1536749516 1.413370e-01 0.3494755885 11 62611597 62612164 568 - 1.078 0.778 -1.132
ENSG00000149499 E048 3.610768 0.0189287939 4.839815e-01 0.6897665180 11 62612165 62612224 60 - 0.473 0.603 0.609
ENSG00000149499 E049 2.688364 0.0376378888 6.854255e-01 0.8261132562 11 62612225 62612381 157 - 0.560 0.484 -0.360
ENSG00000149499 E050 11.713861 0.0027583863 7.761377e-04 0.0080822931 11 62612436 62612573 138 - 0.560 1.070 2.030
ENSG00000149499 E051 4.458910 0.0057828797 2.194143e-01 0.4527310972 11 62612574 62612775 202 - 0.473 0.694 0.999