ENSG00000149100

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323213 ENSG00000149100 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF3M protein_coding protein_coding 178.5359 91.63698 234.7823 8.745625 11.38336 1.357226 77.05071 39.522841 101.77728 3.492653 6.8235059 1.3644338 0.4350958 0.4339333 0.4333333 -0.00060000 1.0000000 0.0112645 FALSE  
ENST00000531120 ENSG00000149100 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF3M protein_coding protein_coding 178.5359 91.63698 234.7823 8.745625 11.38336 1.357226 17.74535 8.797913 24.27636 1.185855 0.4764023 1.4632744 0.1014625 0.0955000 0.1037000 0.00820000 0.8827567 0.0112645 FALSE  
ENST00000531186 ENSG00000149100 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF3M protein_coding retained_intron 178.5359 91.63698 234.7823 8.745625 11.38336 1.357226 37.21961 22.599069 41.46943 2.286027 2.5222288 0.8754946 0.2071250 0.2468000 0.1764333 -0.07036667 0.0112645 0.0112645 FALSE  
ENST00000532444 ENSG00000149100 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF3M protein_coding nonsense_mediated_decay 178.5359 91.63698 234.7823 8.745625 11.38336 1.357226 33.58610 15.245853 46.71035 3.924450 5.6015522 1.6146883 0.1845583 0.1628333 0.1988667 0.03603333 0.8116678 0.0112645 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000149100 E001 37.128781 0.0009599682 0.5832808118 0.759160457 11 32583798 32583830 33 + 1.566 1.531 -0.118
ENSG00000149100 E002 50.398627 0.0008229919 0.2646565788 0.503949926 11 32583831 32583831 1 + 1.703 1.642 -0.207
ENSG00000149100 E003 54.463552 0.0011428303 0.4299161766 0.650994239 11 32583832 32583833 2 + 1.729 1.686 -0.144
ENSG00000149100 E004 60.047896 0.0008664207 0.6874441385 0.827447089 11 32583834 32583837 4 + 1.752 1.770 0.064
ENSG00000149100 E005 60.779042 0.0007442848 0.6559796452 0.807343107 11 32583838 32583838 1 + 1.757 1.777 0.070
ENSG00000149100 E006 179.049045 0.0002756608 0.0168027980 0.084731465 11 32583839 32583847 9 + 2.208 2.275 0.223
ENSG00000149100 E007 400.714323 0.0013123726 0.0005501363 0.006132716 11 32583848 32583883 36 + 2.549 2.635 0.285
ENSG00000149100 E008 551.535613 0.0008588723 0.0008705463 0.008852622 11 32583884 32583929 46 + 2.693 2.761 0.227
ENSG00000149100 E009 1.822139 0.0125550937 0.6014890849 0.771139503 11 32583976 32584068 93 + 0.475 0.382 -0.495
ENSG00000149100 E010 2.517866 0.0082827058 0.9549303584 0.979366453 11 32584069 32584286 218 + 0.535 0.526 -0.045
ENSG00000149100 E011 15.049361 0.0023055336 0.7817296743 0.885646000 11 32586934 32587011 78 + 1.192 1.166 -0.093
ENSG00000149100 E012 500.115247 0.0001651384 0.0004825470 0.005516869 11 32587012 32587045 34 + 2.654 2.713 0.197
ENSG00000149100 E013 876.477518 0.0004379174 0.9059976283 0.955119439 11 32587046 32587144 99 + 2.917 2.914 -0.009
ENSG00000149100 E014 1068.143390 0.0001250019 0.5056897848 0.704723979 11 32588594 32588732 139 + 3.001 3.008 0.024
ENSG00000149100 E015 3.721043 0.1074781711 0.7173832328 0.846510186 11 32588733 32588927 195 + 0.613 0.689 0.326
ENSG00000149100 E016 979.974363 0.0003447896 0.5872123833 0.761771671 11 32589012 32589135 124 + 2.963 2.970 0.023
ENSG00000149100 E017 689.774276 0.0001419932 0.4756414963 0.683958931 11 32589547 32589585 39 + 2.817 2.805 -0.039
ENSG00000149100 E018 867.972241 0.0004967734 0.3676536542 0.602129832 11 32589586 32589641 56 + 2.917 2.900 -0.057
ENSG00000149100 E019 6.084442 0.0379926176 0.3068965503 0.547522784 11 32593769 32593865 97 + 0.766 0.912 0.571
ENSG00000149100 E020 985.172077 0.0003576667 0.6949928292 0.832310336 11 32593866 32593949 84 + 2.965 2.968 0.011
ENSG00000149100 E021 150.052570 0.0283770422 0.0288775272 0.124015484 11 32593950 32594913 964 + 2.203 2.014 -0.635
ENSG00000149100 E022 1064.388636 0.0006640858 0.2826884900 0.523111705 11 32594914 32595013 100 + 2.994 3.010 0.050
ENSG00000149100 E023 923.740439 0.0002429363 0.5364388509 0.726628979 11 32595966 32596047 82 + 2.937 2.944 0.025
ENSG00000149100 E024 1399.914920 0.0003464765 0.0204579440 0.097525080 11 32600689 32600832 144 + 3.128 3.098 -0.102
ENSG00000149100 E025 5.126496 0.0231379836 0.7610390680 0.873191831 11 32600833 32601501 669 + 0.769 0.720 -0.199
ENSG00000149100 E026 984.204371 0.0001811427 0.0006144618 0.006707144 11 32601762 32601822 61 + 2.980 2.934 -0.154
ENSG00000149100 E027 4.016003 0.0804630270 0.1758594593 0.398274091 11 32602079 32602114 36 + 0.740 0.461 -1.246
ENSG00000149100 E028 991.862229 0.0004390928 0.0420370167 0.160020898 11 32602279 32606264 3986 + 2.979 2.946 -0.108