ENSG00000148516

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000320985 ENSG00000148516 HEK293_OSMI2_6hA HEK293_TMG_6hB ZEB1 protein_coding protein_coding 3.822067 0.9808787 7.402796 0.2348082 0.3976449 2.903238 0.5118528 0.4708182 0.71347881 0.08785243 0.25454515 0.5894593 0.17318750 0.4962000 0.100566667 -0.395633333 1.315890e-02 1.317465e-05 FALSE TRUE
ENST00000424869 ENSG00000148516 HEK293_OSMI2_6hA HEK293_TMG_6hB ZEB1 protein_coding protein_coding 3.822067 0.9808787 7.402796 0.2348082 0.3976449 2.903238 1.8067700 0.2933359 4.17215288 0.19637516 0.33251326 3.7852575 0.35043333 0.2819000 0.561966667 0.280066667 5.857162e-01 1.317465e-05 FALSE TRUE
ENST00000542815 ENSG00000148516 HEK293_OSMI2_6hA HEK293_TMG_6hB ZEB1 protein_coding protein_coding 3.822067 0.9808787 7.402796 0.2348082 0.3976449 2.903238 0.1633879 0.1338430 0.00000000 0.13384303 0.00000000 -3.8464234 0.19216250 0.1383667 0.000000000 -0.138366667 6.404373e-01 1.317465e-05 FALSE TRUE
ENST00000559858 ENSG00000148516 HEK293_OSMI2_6hA HEK293_TMG_6hB ZEB1 protein_coding processed_transcript 3.822067 0.9808787 7.402796 0.2348082 0.3976449 2.903238 0.7876215 0.0000000 2.23819155 0.00000000 0.45182660 7.8126212 0.11484583 0.0000000 0.297400000 0.297400000 1.317465e-05 1.317465e-05 FALSE TRUE
ENST00000560721 ENSG00000148516 HEK293_OSMI2_6hA HEK293_TMG_6hB ZEB1 protein_coding protein_coding 3.822067 0.9808787 7.402796 0.2348082 0.3976449 2.903238 0.1036771 0.0000000 0.04474911 0.00000000 0.04474911 2.4528355 0.07263333 0.0000000 0.006733333 0.006733333 1.000000e+00 1.317465e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000148516 E001 0.0000000       10 31318495 31318580 86 +      
ENSG00000148516 E002 0.0000000       10 31318783 31318817 35 +      
ENSG00000148516 E003 0.0000000       10 31318818 31318836 19 +      
ENSG00000148516 E004 0.0000000       10 31318837 31318912 76 +      
ENSG00000148516 E005 0.0000000       10 31318913 31319124 212 +      
ENSG00000148516 E006 0.0000000       10 31319125 31319133 9 +      
ENSG00000148516 E007 0.0000000       10 31319134 31319211 78 +      
ENSG00000148516 E008 0.0000000       10 31319212 31319212 1 +      
ENSG00000148516 E009 0.1614157 0.0339885699 9.767891e-01   10 31319213 31319215 3 + 0.052 0.000 -9.097
ENSG00000148516 E010 0.1614157 0.0339885699 9.767891e-01   10 31319216 31319221 6 + 0.052 0.000 -10.564
ENSG00000148516 E011 9.9803590 0.0044405122 4.836737e-01 6.895651e-01 10 31319222 31319292 71 + 0.911 0.826 -0.327
ENSG00000148516 E012 0.0000000       10 31320113 31320125 13 +      
ENSG00000148516 E013 0.0000000       10 31320126 31320207 82 +      
ENSG00000148516 E014 0.0000000       10 31320547 31320605 59 +      
ENSG00000148516 E015 0.1779838 0.0353963150 9.803932e-01   10 31320606 31320698 93 + 0.052 0.000 -10.554
ENSG00000148516 E016 0.0000000       10 31321135 31321151 17 +      
ENSG00000148516 E017 0.1779838 0.0353963150 9.803932e-01   10 31321152 31321473 322 + 0.052 0.000 -10.554
ENSG00000148516 E018 0.1779838 0.0353963150 9.803932e-01   10 31321474 31321532 59 + 0.052 0.000 -10.554
ENSG00000148516 E019 0.0000000       10 31321621 31321689 69 +      
ENSG00000148516 E020 0.0000000       10 31321690 31321851 162 +      
ENSG00000148516 E021 0.0000000       10 31322010 31322084 75 +      
ENSG00000148516 E022 0.0000000       10 31355144 31355146 3 +      
ENSG00000148516 E023 0.2027342 0.0392975471 5.681634e-02   10 31355147 31355192 46 + 0.000 0.259 13.230
ENSG00000148516 E024 0.0000000       10 31358145 31358590 446 +      
ENSG00000148516 E025 0.3206185 0.0274424043 1.000000e+00   10 31373018 31373173 156 + 0.099 0.000 -11.581
ENSG00000148516 E026 0.0000000       10 31374803 31374964 162 +      
ENSG00000148516 E027 0.0000000       10 31381723 31381829 107 +      
ENSG00000148516 E028 1.2515505 0.0148500414 2.715550e-01   10 31387124 31387266 143 + 0.306 0.000 -13.513
ENSG00000148516 E029 0.4466850 0.0236368753 1.000000e+00   10 31387720 31387798 79 + 0.141 0.000 -12.164
ENSG00000148516 E030 0.0000000       10 31389605 31389660 56 +      
ENSG00000148516 E031 0.0000000       10 31389661 31389695 35 +      
ENSG00000148516 E032 35.6552963 0.0010024493 1.743625e-01 3.963481e-01 10 31461037 31461237 201 + 1.424 1.330 -0.328
ENSG00000148516 E033 9.1638810 0.0032155417 2.817296e-02 1.217780e-01 10 31495776 31495778 3 + 0.904 0.537 -1.524
ENSG00000148516 E034 26.4579850 0.0013618585 5.597633e-01 7.431055e-01 10 31495779 31495838 60 + 1.293 1.258 -0.125
ENSG00000148516 E035 15.1091749 0.0027392770 6.453608e-01 8.004112e-01 10 31502348 31502353 6 + 1.069 1.032 -0.135
ENSG00000148516 E036 30.7842700 0.0013718832 4.523347e-01 6.674292e-01 10 31502354 31502509 156 + 1.359 1.312 -0.161
ENSG00000148516 E037 36.7029475 0.0010311338 1.917297e-02 9.305550e-02 10 31510673 31510875 203 + 1.444 1.258 -0.649
ENSG00000148516 E038 28.0622858 0.0047177538 4.184613e-01 6.424720e-01 10 31514603 31514708 106 + 1.320 1.258 -0.216
ENSG00000148516 E039 197.5454791 0.0003451211 1.344689e-02 7.217873e-02 10 31520126 31521936 1811 + 2.148 2.090 -0.194
ENSG00000148516 E040 49.5102902 0.0032540932 3.338291e-01 5.724252e-01 10 31523933 31524113 181 + 1.555 1.504 -0.175
ENSG00000148516 E041 215.3900339 0.0028578680 7.047870e-08 2.713422e-06 10 31526672 31529814 3143 + 2.146 2.327 0.603