ENSG00000148484

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000345264 ENSG00000148484 HEK293_OSMI2_6hA HEK293_TMG_6hB RSU1 protein_coding protein_coding 52.10475 56.97391 41.96429 6.684571 1.416914 -0.4410489 3.48073 1.122767 4.429429 0.3732919 0.1251387 1.9705232 0.0712250 0.0187000 0.1056000 0.08690000 0.0001047122 2.805364e-06 FALSE TRUE
ENST00000377911 ENSG00000148484 HEK293_OSMI2_6hA HEK293_TMG_6hB RSU1 protein_coding processed_transcript 52.10475 56.97391 41.96429 6.684571 1.416914 -0.4410489 28.90528 31.779509 22.648532 3.8709131 1.1333647 -0.4884964 0.5560042 0.5571333 0.5391000 -0.01803333 0.8768140101 2.805364e-06 FALSE TRUE
MSTRG.3609.4 ENSG00000148484 HEK293_OSMI2_6hA HEK293_TMG_6hB RSU1 protein_coding   52.10475 56.97391 41.96429 6.684571 1.416914 -0.4410489 16.95341 19.633932 11.882613 2.8562440 0.7104242 -0.7240180 0.3176333 0.3426000 0.2826667 -0.05993333 0.3563243596 2.805364e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000148484 E001 0.0000000       10 16590611 16590619 9 -      
ENSG00000148484 E002 113.2285235 0.0038921026 1.971155e-44 4.714752e-41 10 16590620 16592552 1933 - 2.324 1.679 -2.166
ENSG00000148484 E003 121.3014737 0.0060953129 1.441920e-01 3.536558e-01 10 16592553 16593040 488 - 2.116 2.044 -0.241
ENSG00000148484 E004 202.9192688 0.0002867794 1.166203e-01 3.101958e-01 10 16593041 16593152 112 - 2.274 2.309 0.115
ENSG00000148484 E005 357.5694451 0.0002497696 5.058042e-01 7.048186e-01 10 16593153 16593312 160 - 2.537 2.544 0.021
ENSG00000148484 E006 404.2246772 0.0006788336 5.635315e-01 7.457703e-01 10 16593313 16593496 184 - 2.590 2.596 0.019
ENSG00000148484 E007 314.1188008 0.0002201601 8.944400e-01 9.489320e-01 10 16695023 16695155 133 - 2.487 2.483 -0.012
ENSG00000148484 E008 0.1723744 0.0338199806 7.010033e-01   10 16727031 16727157 127 - 0.000 0.101 8.565
ENSG00000148484 E009 332.6414527 0.0002389860 5.815151e-01 7.581523e-01 10 16752539 16752653 115 - 2.508 2.513 0.017
ENSG00000148484 E010 307.3575202 0.0020453172 5.274894e-01 7.202398e-01 10 16752918 16753000 83 - 2.471 2.484 0.045
ENSG00000148484 E011 384.9122085 0.0004491312 2.945631e-01 5.351302e-01 10 16754871 16754989 119 - 2.566 2.581 0.050
ENSG00000148484 E012 405.9630763 0.0001553466 2.326703e-03 1.922670e-02 10 16764390 16764510 121 - 2.567 2.615 0.159
ENSG00000148484 E013 263.2538046 0.0004382090 2.925086e-01 5.331733e-01 10 16782034 16782084 51 - 2.397 2.415 0.060
ENSG00000148484 E014 231.6808263 0.0003389623 3.460822e-01 5.836359e-01 10 16816973 16817084 112 - 2.342 2.359 0.055
ENSG00000148484 E015 7.8121844 0.0040691051 7.444876e-01 8.633761e-01 10 16817085 16817314 230 - 0.913 0.945 0.120
ENSG00000148484 E016 211.3270957 0.0002295845 5.111153e-02 1.823320e-01 10 16817315 16817478 164 - 2.288 2.330 0.141