ENSG00000148400

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000651671 ENSG00000148400 HEK293_OSMI2_6hA HEK293_TMG_6hB NOTCH1 protein_coding protein_coding 6.689768 8.830747 5.565171 0.4882463 0.07139935 -0.6651523 4.4654008 5.3106495 4.204370 0.13685756 0.5774990 -0.3362853 0.66923750 0.60470000 0.7575333 0.15283333 6.345790e-01 6.184415e-16 FALSE TRUE
ENST00000680778 ENSG00000148400 HEK293_OSMI2_6hA HEK293_TMG_6hB NOTCH1 protein_coding protein_coding 6.689768 8.830747 5.565171 0.4882463 0.07139935 -0.6651523 1.2847506 2.1142957 0.000000 0.04718236 0.0000000 -7.7308408 0.19074167 0.24140000 0.0000000 -0.24140000 6.184415e-16 6.184415e-16 FALSE TRUE
ENST00000681454 ENSG00000148400 HEK293_OSMI2_6hA HEK293_TMG_6hB NOTCH1 protein_coding nonsense_mediated_decay 6.689768 8.830747 5.565171 0.4882463 0.07139935 -0.6651523 0.2647166 0.4138728 0.722564 0.41387283 0.4187656 0.7893233 0.03992083 0.04233333 0.1287667 0.08643333 6.891038e-01 6.184415e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000148400 E001 0.0000000       9 136494433 136494443 11 -      
ENSG00000148400 E002 2.8277476 0.1464369322 5.262262e-01 0.7193709028 9 136494444 136494456 13 - 0.649 0.527 -0.546
ENSG00000148400 E003 236.7429124 0.0050059417 2.012076e-04 0.0026886200 9 136494457 136495462 1006 - 2.257 2.404 0.491
ENSG00000148400 E004 71.6932172 0.0005635621 5.353788e-02 0.1879592311 9 136495463 136495688 226 - 1.783 1.869 0.292
ENSG00000148400 E005 31.7167170 0.0012132943 3.034814e-01 0.5440355340 9 136495689 136495735 47 - 1.448 1.516 0.234
ENSG00000148400 E006 139.3824209 0.0007374291 1.666101e-01 0.3860185774 9 136495736 136496229 494 - 2.096 2.142 0.155
ENSG00000148400 E007 291.3987905 0.0001937073 8.626868e-05 0.0013208817 9 136496230 136497558 1329 - 2.389 2.475 0.286
ENSG00000148400 E008 50.4864191 0.0008920443 8.295743e-01 0.9135260692 9 136498899 136498996 98 - 1.698 1.688 -0.034
ENSG00000148400 E009 0.3447487 0.2270608567 4.702329e-01   9 136498997 136499111 115 - 0.000 0.173 10.862
ENSG00000148400 E010 64.8055431 0.0007737314 2.705071e-01 0.5104890317 9 136499112 136499259 148 - 1.764 1.817 0.178
ENSG00000148400 E011 6.8529437 0.2710166569 1.960936e-01 0.4246650223 9 136499260 136500551 1292 - 0.989 0.796 -0.735
ENSG00000148400 E012 98.5958942 0.0003980269 6.226985e-02 0.2078840438 9 136500552 136500847 296 - 1.932 2.003 0.239
ENSG00000148400 E013 73.8092876 0.0006071518 2.231951e-01 0.4571958656 9 136501748 136501913 166 - 1.821 1.875 0.181
ENSG00000148400 E014 50.8471070 0.0007872472 5.753441e-01 0.7539036112 9 136502001 136502088 88 - 1.679 1.709 0.102
ENSG00000148400 E015 54.2633977 0.0007003549 6.466786e-01 0.8012849161 9 136502272 136502436 165 - 1.709 1.733 0.081
ENSG00000148400 E016 29.3304901 0.0015220870 3.749454e-01 0.6081360092 9 136502437 136502488 52 - 1.420 1.482 0.213
ENSG00000148400 E017 1.5413612 0.1111643141 1.936203e-01 0.4216078243 9 136502489 136502775 287 - 0.549 0.295 -1.389
ENSG00000148400 E018 2.4862006 0.2692495529 6.532679e-01 0.8056112466 9 136502776 136503181 406 - 0.634 0.471 -0.753
ENSG00000148400 E019 47.9483488 0.0007479627 3.045571e-02 0.1287099293 9 136503182 136503330 149 - 1.593 1.711 0.402
ENSG00000148400 E020 91.9078309 0.0004624021 7.918995e-02 0.2432052154 9 136504673 136505104 432 - 1.903 1.973 0.235
ENSG00000148400 E021 114.3143894 0.0004863225 6.726426e-01 0.8179860924 9 136505310 136505881 572 - 2.029 2.045 0.053
ENSG00000148400 E022 34.6372319 0.0010170018 5.911655e-01 0.7644974242 9 136506527 136506639 113 - 1.509 1.544 0.117
ENSG00000148400 E023 41.7909523 0.0010382420 7.719160e-01 0.8797750573 9 136506716 136506835 120 - 1.598 1.616 0.061
ENSG00000148400 E024 56.3302131 0.0151619755 7.918402e-01 0.8915157668 9 136506836 136506973 138 - 1.747 1.728 -0.066
ENSG00000148400 E025 54.8657102 0.0011035396 2.976095e-01 0.5380989629 9 136507305 136507437 133 - 1.757 1.706 -0.175
ENSG00000148400 E026 58.8930096 0.0008064047 8.364574e-01 0.9175092983 9 136507955 136508139 185 - 1.767 1.758 -0.030
ENSG00000148400 E027 30.4747714 0.0013222552 9.347717e-01 0.9695899599 9 136508232 136508292 61 - 1.480 1.476 -0.014
ENSG00000148400 E028 37.5832611 0.0012985149 5.707421e-01 0.7508105735 9 136508293 136508385 93 - 1.593 1.561 -0.110
ENSG00000148400 E029 61.8878905 0.0005764767 6.577637e-01 0.8084900234 9 136508870 136509071 202 - 1.792 1.773 -0.066
ENSG00000148400 E030 0.3751086 0.0274424043 4.007798e-01   9 136509072 136509343 272 - 0.000 0.172 11.818
ENSG00000148400 E031 73.4340910 0.0006068704 6.718509e-01 0.8174816785 9 136509733 136509961 229 - 1.864 1.846 -0.059
ENSG00000148400 E032 0.0000000       9 136510510 136510652 143 -      
ENSG00000148400 E033 61.1110521 0.0208982620 3.945524e-01 0.6239937792 9 136510653 136510800 148 - 1.824 1.747 -0.259
ENSG00000148400 E034 24.0077743 0.0661219504 5.677749e-01 0.7486179437 9 136510801 136510805 5 - 1.438 1.347 -0.316
ENSG00000148400 E035 56.0351431 0.0174961693 1.415596e-01 0.3498087379 9 136511152 136511271 120 - 1.804 1.695 -0.368
ENSG00000148400 E036 0.0000000       9 136512693 136512773 81 -      
ENSG00000148400 E037 0.0000000       9 136512774 136512848 75 -      
ENSG00000148400 E038 60.8239080 0.0015501798 2.692307e-02 0.1180855971 9 136513021 136513134 114 - 1.840 1.735 -0.354
ENSG00000148400 E039 65.1891789 0.0032037941 1.236374e-01 0.3214731913 9 136513392 136513537 146 - 1.853 1.778 -0.252
ENSG00000148400 E040 0.3447487 0.2270608567 4.702329e-01   9 136513538 136513551 14 - 0.000 0.173 10.862
ENSG00000148400 E041 69.9354708 0.0017121394 1.223123e-01 0.3194722354 9 136514510 136514702 193 - 1.879 1.808 -0.240
ENSG00000148400 E042 60.7980286 0.0082337154 3.638606e-01 0.5989200803 9 136515290 136515400 111 - 1.812 1.753 -0.199
ENSG00000148400 E043 81.6881905 0.0005871830 1.736551e-02 0.0867932870 9 136515483 136515716 234 - 1.957 1.862 -0.320
ENSG00000148400 E044 47.6465589 0.0012138021 1.228899e-01 0.3202960726 9 136515981 136516094 114 - 1.713 1.631 -0.277
ENSG00000148400 E045 40.6071908 0.0044249862 3.354199e-03 0.0255034741 9 136517272 136517385 114 - 1.709 1.526 -0.624
ENSG00000148400 E046 46.8346390 0.0036686036 1.038417e-02 0.0597928750 9 136517752 136517937 186 - 1.748 1.603 -0.491
ENSG00000148400 E047 47.4023695 0.0008502435 1.703863e-02 0.0856100026 9 136518137 136518292 156 - 1.741 1.618 -0.417
ENSG00000148400 E048 63.9529664 0.0006237585 5.909286e-05 0.0009611248 9 136518591 136518824 234 - 1.903 1.725 -0.599
ENSG00000148400 E049 45.3447366 0.0007591813 4.080037e-04 0.0048083556 9 136519443 136519565 123 - 1.757 1.573 -0.627
ENSG00000148400 E050 5.0245063 0.0148909586 6.758168e-02 0.2196473361 9 136522572 136522849 278 - 0.554 0.835 1.178
ENSG00000148400 E051 65.2142569 0.0007951638 5.837794e-02 0.1991466095 9 136522850 136523188 339 - 1.859 1.774 -0.285
ENSG00000148400 E052 39.7078588 0.0099964827 5.977782e-01 0.7688105720 9 136523717 136523979 263 - 1.621 1.581 -0.137
ENSG00000148400 E053 14.3105574 0.0024798345 2.402905e-01 0.4769160685 9 136544024 136544102 79 - 1.100 1.211 0.399
ENSG00000148400 E054 6.9900628 0.0046170334 2.827083e-02 0.1220711442 9 136545726 136546048 323 - 0.687 0.977 1.135