ENSG00000148290

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371974 ENSG00000148290 HEK293_OSMI2_6hA HEK293_TMG_6hB SURF1 protein_coding protein_coding 37.27105 45.55277 21.24955 5.341832 0.4965427 -1.099745 13.936847 19.959848 2.963883 4.4818631 0.4312499 -2.7474036 0.35552917 0.42666667 0.1387333 -0.28793333 0.000251471 0.000251471 FALSE TRUE
ENST00000437995 ENSG00000148290 HEK293_OSMI2_6hA HEK293_TMG_6hB SURF1 protein_coding retained_intron 37.27105 45.55277 21.24955 5.341832 0.4965427 -1.099745 3.388343 3.815700 2.807156 2.0767153 1.0610366 -0.4414844 0.09692083 0.09483333 0.1299333 0.03510000 0.891938306 0.000251471 FALSE TRUE
ENST00000495952 ENSG00000148290 HEK293_OSMI2_6hA HEK293_TMG_6hB SURF1 protein_coding processed_transcript 37.27105 45.55277 21.24955 5.341832 0.4965427 -1.099745 4.309283 3.745477 3.274921 0.9281719 0.3125952 -0.1931375 0.12724167 0.09023333 0.1549667 0.06473333 0.540247782 0.000251471 FALSE TRUE
MSTRG.33591.2 ENSG00000148290 HEK293_OSMI2_6hA HEK293_TMG_6hB SURF1 protein_coding   37.27105 45.55277 21.24955 5.341832 0.4965427 -1.099745 14.710761 16.813108 11.657834 4.1592968 0.7187439 -0.5279074 0.39411667 0.36053333 0.5507000 0.19016667 0.341028351 0.000251471 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000148290 E001 0.6597577 0.7197993890 9.232801e-01   9 133351758 133351802 45 - 0.187 0.201 0.124
ENSG00000148290 E002 0.8928518 0.1421834594 7.505057e-01   9 133351803 133351804 2 - 0.189 0.250 0.509
ENSG00000148290 E003 55.3294972 0.0010030958 8.259302e-01 9.114563e-01 9 133351805 133351874 70 - 1.688 1.696 0.025
ENSG00000148290 E004 99.9253753 0.0006708159 6.177629e-01 7.822692e-01 9 133351875 133351912 38 - 1.935 1.951 0.053
ENSG00000148290 E005 175.2388075 0.0007608521 7.439128e-01 8.629220e-01 9 133351913 133351982 70 - 2.199 2.183 -0.053
ENSG00000148290 E006 264.4261671 0.0003082309 4.883287e-01 6.928769e-01 9 133352061 133352142 82 - 2.382 2.361 -0.071
ENSG00000148290 E007 384.0774081 0.0004645335 1.204686e-01 3.165748e-01 9 133352446 133352608 163 - 2.553 2.516 -0.123
ENSG00000148290 E008 204.0153040 0.0005150787 6.348488e-01 7.934626e-01 9 133352694 133352730 37 - 2.266 2.247 -0.062
ENSG00000148290 E009 187.0590477 0.0065533720 2.716763e-01 5.115270e-01 9 133352731 133352766 36 - 2.252 2.199 -0.178
ENSG00000148290 E010 392.9510682 0.0002534338 9.948435e-01 9.989823e-01 9 133353749 133353940 192 - 2.541 2.536 -0.017
ENSG00000148290 E011 23.2205600 0.1135133766 1.290407e-01 3.302699e-01 9 133353941 133354253 313 - 1.497 1.245 -0.874
ENSG00000148290 E012 232.9873885 0.0032174130 6.046838e-01 7.733004e-01 9 133354659 133354741 83 - 2.330 2.302 -0.093
ENSG00000148290 E013 210.1509895 0.0003149746 5.814333e-01 7.581097e-01 9 133354824 133354957 134 - 2.259 2.269 0.034
ENSG00000148290 E014 10.1971498 0.0611741342 5.771879e-01 7.552251e-01 9 133356105 133356268 164 - 1.061 0.959 -0.373
ENSG00000148290 E015 83.0582024 0.0008781868 4.157252e-05 7.111888e-04 9 133356269 133356320 52 - 1.717 1.910 0.651
ENSG00000148290 E016 42.9829856 0.0050610036 3.289839e-09 1.716934e-07 9 133356400 133356458 59 - 1.184 1.672 1.688
ENSG00000148290 E017 20.4067988 0.0018542080 4.825667e-05 8.069213e-04 9 133356459 133356676 218 - 0.939 1.350 1.476