ENSG00000146278

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000336032 ENSG00000146278 HEK293_OSMI2_6hA HEK293_TMG_6hB PNRC1 protein_coding protein_coding 16.96099 19.56115 21.24713 3.375846 1.774744 0.1192184 13.710440 17.878589 16.197022 3.26152423 0.3889791 -0.1424206 0.81222917 0.90993333 0.77130000 -0.13863333 0.130661523 0.007640469 FALSE  
ENST00000354922 ENSG00000146278 HEK293_OSMI2_6hA HEK293_TMG_6hB PNRC1 protein_coding protein_coding 16.96099 19.56115 21.24713 3.375846 1.774744 0.1192184 1.667334 1.189731 3.078953 0.04961886 0.2321054 1.3644076 0.09657917 0.06423333 0.14520000 0.08096667 0.007640469 0.007640469 FALSE  
ENST00000369472 ENSG00000146278 HEK293_OSMI2_6hA HEK293_TMG_6hB PNRC1 protein_coding protein_coding 16.96099 19.56115 21.24713 3.375846 1.774744 0.1192184 1.096564 0.000000 1.226759 0.00000000 1.2267592 6.9504208 0.06360833 0.00000000 0.04993333 0.04993333 0.897036752 0.007640469 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000146278 E001 53.388737 0.0092490164 0.8484346615 0.924040067 6 89080751 89080771 21 + 1.747 1.710 -0.123
ENSG00000146278 E002 151.891872 0.0003381365 0.0390650630 0.152362326 6 89080772 89080973 202 + 2.157 2.192 0.116
ENSG00000146278 E003 112.083913 0.0006535637 0.0004863934 0.005551874 6 89080974 89081078 105 + 1.983 2.082 0.332
ENSG00000146278 E004 134.057253 0.0022744283 0.0002566570 0.003290274 6 89081079 89081434 356 + 2.047 2.164 0.393
ENSG00000146278 E005 5.359353 0.0066652982 0.0108582511 0.061766623 6 89081836 89081923 88 + 0.993 0.649 -1.355
ENSG00000146278 E006 7.234129 0.0124368666 0.0297635258 0.126629202 6 89081924 89082003 80 + 1.075 0.791 -1.074
ENSG00000146278 E007 14.408092 0.0028856001 0.0002780998 0.003509791 6 89082004 89082165 162 + 1.375 1.036 -1.204
ENSG00000146278 E008 25.290848 0.0435557722 0.0016041740 0.014360589 6 89082166 89082545 380 + 1.626 1.204 -1.460
ENSG00000146278 E009 10.983535 0.2072863238 0.2063654735 0.437419386 6 89082546 89082550 5 + 1.217 0.942 -1.001
ENSG00000146278 E010 29.813319 0.0826031896 0.0185184408 0.090783750 6 89082551 89082735 185 + 1.684 1.284 -1.373
ENSG00000146278 E011 601.343781 0.0023561616 0.7476688500 0.865310711 6 89083753 89085160 1408 + 2.783 2.759 -0.078