ENSG00000145743

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000481160 ENSG00000145743 HEK293_OSMI2_6hA HEK293_TMG_6hB FBXL17 protein_coding retained_intron 3.418079 1.806868 6.9786 0.543657 0.2266572 1.943549 1.003936 0.1614865 2.843187 0.1614865 0.1536585 4.056408 0.1724125 0.05956667 0.4074333 0.3478667 0.1017948 0.0356188 FALSE TRUE
ENST00000542267 ENSG00000145743 HEK293_OSMI2_6hA HEK293_TMG_6hB FBXL17 protein_coding protein_coding 3.418079 1.806868 6.9786 0.543657 0.2266572 1.943549 2.391088 1.6005737 4.102308 0.4267376 0.1842533 1.352374 0.8142542 0.90496667 0.5877333 -0.3172333 0.0356188 0.0356188 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000145743 E001 225.2511743 0.0329023285 0.300347319 0.54078631 5 107859035 107861860 2826 - 2.274 2.384 0.369
ENSG00000145743 E002 0.6494192 0.0217889718 0.889814548   5 107878237 107881036 2800 - 0.202 0.184 -0.167
ENSG00000145743 E003 32.8509337 0.0012329751 0.211272027 0.44329755 5 107881037 107881179 143 - 1.497 1.468 -0.099
ENSG00000145743 E004 0.1308682 0.0326491905 0.199320048   5 108020799 108020924 126 - 0.000 0.183 10.101
ENSG00000145743 E005 30.3494140 0.0015177139 0.021485339 0.10092957 5 108020925 108021001 77 - 1.483 1.373 -0.380
ENSG00000145743 E006 42.9665125 0.0012082095 0.237752989 0.47397898 5 108186117 108186247 131 - 1.608 1.595 -0.043
ENSG00000145743 E007 38.2631464 0.0052310759 0.042101909 0.16016604 5 108224121 108224228 108 - 1.573 1.489 -0.289
ENSG00000145743 E008 43.3775919 0.0009432135 0.022254167 0.10345093 5 108348399 108348530 132 - 1.626 1.547 -0.272
ENSG00000145743 E009 30.8283500 0.0034603391 0.019844271 0.09534361 5 108364738 108364822 85 - 1.494 1.373 -0.417
ENSG00000145743 E010 34.1444588 0.0157494331 0.159851946 0.37655491 5 108364823 108364995 173 - 1.528 1.454 -0.254
ENSG00000145743 E011 20.6023811 0.0021700814 0.060120574 0.20309759 5 108367831 108367953 123 - 1.231 1.428 0.687
ENSG00000145743 E012 0.0000000       5 108367954 108367954 1 -      
ENSG00000145743 E013 47.9097367 0.0177458656 0.003529421 0.02651752 5 108380699 108382098 1400 - 1.556 1.826 0.917