ENSG00000145390

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000274030 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding protein_coding 5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 0.28979651 0.06461014 0.5437602 0.06461014 0.27576895 2.8918177 0.05035417 0.06590000 0.04273333 -0.02316667 9.803910e-01 1.039478e-06 FALSE TRUE
ENST00000450251 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding protein_coding 5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 0.28446392 0.14572214 1.1101844 0.07606074 0.58056451 2.8466903 0.05650000 0.12776667 0.08903333 -0.03873333 9.591778e-01 1.039478e-06 FALSE TRUE
ENST00000509769 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding nonsense_mediated_decay 5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 0.13553426 0.12735257 0.3944560 0.12735257 0.22723607 1.5580990 0.03538750 0.10903333 0.03113333 -0.07790000 1.000000e+00 1.039478e-06 FALSE TRUE
ENST00000514305 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding processed_transcript 5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 0.09843071 0.07442162 0.1323989 0.02249190 0.06346175 0.7542537 0.04253333 0.06653333 0.01060000 -0.05593333 1.354532e-01 1.039478e-06   FALSE
ENST00000688980 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding protein_coding 5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 1.29180340 0.00000000 2.6718166 0.00000000 0.07918039 8.0670667 0.12966250 0.00000000 0.21190000 0.21190000 1.039478e-06 1.039478e-06 FALSE TRUE
ENST00000692078 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding protein_coding 5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 0.48374767 0.11829095 0.7401052 0.03716808 0.13458945 2.5476734 0.10813333 0.10996667 0.05843333 -0.05153333 7.012445e-01 1.039478e-06 FALSE TRUE
MSTRG.25381.1 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding   5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 2.52293983 0.14459021 4.5816747 0.14459021 0.63825254 4.8924996 0.42264167 0.12380000 0.36323333 0.23943333 3.953846e-01 1.039478e-06 FALSE TRUE
MSTRG.25381.3 ENSG00000145390 HEK293_OSMI2_6hA HEK293_TMG_6hB USP53 protein_coding   5.691053 1.090041 12.6168 0.05645899 0.1430629 3.52086 0.23541478 0.30520717 1.1648402 0.16579797 0.58880608 1.8980923 0.09707500 0.28686667 0.09236667 -0.19450000 8.612078e-01 1.039478e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000145390 E001 0.3206185 0.0274424043 1.000000e+00   4 119212587 119212673 87 + 0.074 0.000 -8.107
ENSG00000145390 E002 4.5576801 0.0059401659 1.975972e-01 0.426602389 4 119212674 119212712 39 + 0.556 0.327 -1.209
ENSG00000145390 E003 4.8782986 0.0061168450 1.647187e-01 0.383641760 4 119212713 119212720 8 + 0.577 0.327 -1.308
ENSG00000145390 E004 11.9512558 0.0026530002 5.807420e-01 0.757662012 4 119212721 119212740 20 + 0.874 0.888 0.054
ENSG00000145390 E005 11.8086212 0.0026789710 6.051803e-01 0.773640830 4 119212741 119212742 2 + 0.869 0.888 0.075
ENSG00000145390 E006 21.9274993 0.0016827517 6.662429e-01 0.813851733 4 119212743 119212844 102 + 1.106 1.161 0.196
ENSG00000145390 E007 17.6914126 0.0019395818 9.456010e-01 0.975138325 4 119212845 119212873 29 + 1.011 1.126 0.415
ENSG00000145390 E008 0.0000000       4 119213179 119213218 40 +      
ENSG00000145390 E009 7.1849040 0.0173694218 4.383650e-01 0.657514544 4 119214070 119214222 153 + 0.656 0.889 0.934
ENSG00000145390 E010 33.1930721 0.0011004762 5.376955e-01 0.727456376 4 119217550 119217673 124 + 1.261 1.429 0.584
ENSG00000145390 E011 4.4692753 0.0069698504 6.801560e-02 0.220621286 4 119217674 119220115 2442 + 0.483 0.889 1.726
ENSG00000145390 E012 4.7420765 0.0075823945 9.910309e-05 0.001483079 4 119220116 119221495 1380 + 0.441 1.125 2.809
ENSG00000145390 E013 5.0148057 0.0083593281 1.099050e-01 0.298859797 4 119235290 119235411 122 + 0.521 0.889 1.542
ENSG00000145390 E014 28.8007361 0.0039699892 1.506450e-01 0.363214177 4 119239218 119239639 422 + 1.219 1.161 -0.208
ENSG00000145390 E015 26.5758170 0.0218926838 5.534699e-01 0.738682680 4 119239640 119239903 264 + 1.180 1.221 0.145
ENSG00000145390 E016 31.4423560 0.0121809195 8.134349e-02 0.247518545 4 119245337 119245429 93 + 1.259 1.160 -0.350
ENSG00000145390 E017 37.9107471 0.0064952088 1.046538e-02 0.060138157 4 119248748 119248882 135 + 1.341 1.161 -0.636
ENSG00000145390 E018 36.5023081 0.0036186835 1.568233e-02 0.080669708 4 119256246 119256359 114 + 1.323 1.161 -0.569
ENSG00000145390 E019 31.7076563 0.0085279529 2.134030e-01 0.445705280 4 119256441 119256523 83 + 1.256 1.224 -0.113
ENSG00000145390 E020 29.7699392 0.0015030600 1.694681e-01 0.389851077 4 119259820 119259925 106 + 1.238 1.193 -0.159
ENSG00000145390 E021 41.3931861 0.0009567787 5.956727e-02 0.201803344 4 119260507 119260653 147 + 1.376 1.303 -0.255
ENSG00000145390 E022 35.4826672 0.0165575540 2.486972e-01 0.486460623 4 119261715 119261792 78 + 1.305 1.279 -0.092
ENSG00000145390 E023 33.9203407 0.0149743015 2.069861e-01 0.438160694 4 119261793 119261864 72 + 1.286 1.251 -0.124
ENSG00000145390 E024 2.8450893 0.0080155114 5.262795e-01 0.719397598 4 119266300 119266333 34 + 0.411 0.327 -0.490
ENSG00000145390 E025 35.3847079 0.0047987970 6.858868e-01 0.826399453 4 119267320 119267380 61 + 1.297 1.370 0.254
ENSG00000145390 E026 33.9271830 0.0174639770 8.484054e-02 0.254327925 4 119267381 119267435 55 + 1.291 1.192 -0.351
ENSG00000145390 E027 28.7380747 0.0466930631 1.584431e-02 0.081227071 4 119267436 119267482 47 + 1.233 0.943 -1.051
ENSG00000145390 E028 52.6765464 0.0332262472 3.025465e-02 0.128108895 4 119268268 119268420 153 + 1.477 1.322 -0.536
ENSG00000145390 E029 61.5088299 0.0301057003 3.846871e-02 0.150786714 4 119269691 119269837 147 + 1.540 1.410 -0.446
ENSG00000145390 E030 128.9419977 0.0244029325 5.883467e-02 0.200137804 4 119271296 119271787 492 + 1.850 1.802 -0.163
ENSG00000145390 E031 84.9727320 0.0143820683 3.585474e-01 0.594619192 4 119271788 119272034 247 + 1.666 1.709 0.147
ENSG00000145390 E032 0.9640683 0.0156273583 7.276802e-01   4 119272035 119273330 1296 + 0.192 0.000 -9.684
ENSG00000145390 E033 40.1339320 0.0012219393 8.877712e-01 0.945454119 4 119273632 119273708 77 + 1.348 1.446 0.339
ENSG00000145390 E034 38.8713664 0.0535539408 6.872909e-01 0.827363869 4 119291165 119291261 97 + 1.329 1.486 0.545
ENSG00000145390 E035 236.6931663 1.7295568935 3.192655e-01 0.558983690 4 119292338 119295518 3181 + 2.049 2.503 1.516