ENSG00000144224

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000272638 ENSG00000144224 HEK293_OSMI2_6hA HEK293_TMG_6hB UBXN4 protein_coding protein_coding 29.07293 9.32788 51.77582 1.758118 2.510661 2.47139 19.1318711 5.4514807 35.9162864 1.3166720 2.1524493 2.7176758 0.61094167 0.57013333 0.69293333 0.12280000 3.501096e-01 1.905961e-06 FALSE  
ENST00000416538 ENSG00000144224 HEK293_OSMI2_6hA HEK293_TMG_6hB UBXN4 protein_coding nonsense_mediated_decay 29.07293 9.32788 51.77582 1.758118 2.510661 2.47139 1.4907011 0.3857485 3.3790791 0.2253926 0.4657342 3.0982376 0.05223333 0.04120000 0.06496667 0.02376667 7.896865e-01 1.905961e-06 TRUE  
ENST00000426921 ENSG00000144224 HEK293_OSMI2_6hA HEK293_TMG_6hB UBXN4 protein_coding nonsense_mediated_decay 29.07293 9.32788 51.77582 1.758118 2.510661 2.47139 2.0466963 0.0000000 3.1997579 0.0000000 0.8544464 8.3263207 0.04238333 0.00000000 0.06046667 0.06046667 1.905961e-06 1.905961e-06 TRUE  
ENST00000490163 ENSG00000144224 HEK293_OSMI2_6hA HEK293_TMG_6hB UBXN4 protein_coding processed_transcript 29.07293 9.32788 51.77582 1.758118 2.510661 2.47139 1.1972615 0.5008065 1.8401935 0.5008065 0.3650546 1.8568275 0.09918333 0.08023333 0.03636667 -0.04386667 6.758317e-01 1.905961e-06 FALSE  
MSTRG.19269.1 ENSG00000144224 HEK293_OSMI2_6hA HEK293_TMG_6hB UBXN4 protein_coding   29.07293 9.32788 51.77582 1.758118 2.510661 2.47139 3.2561804 1.2501032 4.6596831 0.1157058 1.2941421 1.8897827 0.10643333 0.14030000 0.09283333 -0.04746667 6.715820e-01 1.905961e-06 FALSE  
MSTRG.19269.2 ENSG00000144224 HEK293_OSMI2_6hA HEK293_TMG_6hB UBXN4 protein_coding   29.07293 9.32788 51.77582 1.758118 2.510661 2.47139 0.8483845 1.0734687 0.8659115 0.5374695 0.6029746 -0.3068004 0.04395833 0.10013333 0.01633333 -0.08380000 7.641844e-01 1.905961e-06 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000144224 E001 6.6211928 0.0365424710 1.231097e-02 6.768449e-02 2 135741698 135741712 15 + 0.692 1.097 1.555
ENSG00000144224 E002 8.3242300 0.0252117282 3.737910e-02 1.479474e-01 2 135741713 135741733 21 + 0.807 1.122 1.175
ENSG00000144224 E003 20.0265689 0.0243476214 3.547228e-02 1.428133e-01 2 135741734 135741785 52 + 1.163 1.418 0.891
ENSG00000144224 E004 48.0458711 0.0019768635 1.587217e-04 2.203613e-03 2 135741786 135741854 69 + 1.528 1.770 0.824
ENSG00000144224 E005 43.3507556 0.0008874637 4.527859e-04 5.246910e-03 2 135741855 135741867 13 + 1.487 1.717 0.782
ENSG00000144224 E006 119.1627051 0.0004863847 4.646656e-01 6.763367e-01 2 135741868 135742011 144 + 1.963 2.007 0.150
ENSG00000144224 E007 6.9980185 0.0050209176 1.620345e-02 8.253696e-02 2 135742523 135742753 231 + 0.730 1.055 1.244
ENSG00000144224 E008 1.1690154 0.0600338418 8.159221e-01   2 135747658 135747765 108 + 0.280 0.229 -0.385
ENSG00000144224 E009 1.9991949 0.0106803581 8.803553e-01 9.414331e-01 2 135748111 135748266 156 + 0.399 0.377 -0.125
ENSG00000144224 E010 133.5668339 0.0003365278 4.303176e-03 3.085536e-02 2 135748267 135748369 103 + 2.037 1.934 -0.344
ENSG00000144224 E011 90.7643990 0.0005545094 1.529086e-02 7.916405e-02 2 135753539 135753567 29 + 1.872 1.766 -0.360
ENSG00000144224 E012 34.5734167 0.2324247644 5.014126e-01 7.018132e-01 2 135753568 135754151 584 + 1.457 1.385 -0.249
ENSG00000144224 E013 9.5611957 0.0699125074 3.015884e-01 5.421070e-01 2 135754152 135754158 7 + 0.952 0.762 -0.737
ENSG00000144224 E014 163.1579575 0.0003306827 1.292370e-09 7.404662e-08 2 135754159 135754277 119 + 2.139 1.913 -0.760
ENSG00000144224 E015 216.2438218 0.0002211303 4.516706e-06 1.054496e-04 2 135755517 135755691 175 + 2.249 2.114 -0.451
ENSG00000144224 E016 165.5613724 0.0002985743 3.384006e-02 1.384376e-01 2 135761818 135761911 94 + 2.123 2.060 -0.209
ENSG00000144224 E017 107.1352848 0.0004191641 2.040295e-01 4.346653e-01 2 135769769 135769823 55 + 1.913 1.980 0.227
ENSG00000144224 E018 161.9011145 0.0002863221 1.089540e-01 2.973213e-01 2 135770571 135770735 165 + 2.091 2.160 0.232
ENSG00000144224 E019 84.5148904 0.0114551155 3.269444e-01 5.660958e-01 2 135772420 135772446 27 + 1.809 1.888 0.265
ENSG00000144224 E020 139.3913879 0.0026876654 8.932572e-01 9.482938e-01 2 135772447 135772547 101 + 2.037 2.057 0.068
ENSG00000144224 E021 124.2608361 0.0061026731 9.938113e-01 9.985524e-01 2 135776249 135776351 103 + 1.988 1.996 0.025
ENSG00000144224 E022 171.9814848 0.0003042168 5.460284e-02 1.904539e-01 2 135778948 135779079 132 + 2.137 2.083 -0.181
ENSG00000144224 E023 0.3393995 0.0274424043 1.000000e+00   2 135780163 135780182 20 + 0.115 0.001 -7.028
ENSG00000144224 E024 254.3333952 0.0003286700 1.491251e-02 7.776476e-02 2 135780183 135780385 203 + 2.306 2.248 -0.193
ENSG00000144224 E025 1.4702327 0.0158301010 7.698444e-01 8.785518e-01 2 135781523 135781561 39 + 0.313 0.377 0.389
ENSG00000144224 E026 245.0012571 0.0017644308 4.708910e-02 1.727435e-01 2 135782749 135782895 147 + 2.291 2.230 -0.201
ENSG00000144224 E027 494.6246565 0.0002111964 3.555724e-01 5.921917e-01 2 135782896 135783508 613 + 2.580 2.612 0.107
ENSG00000144224 E028 942.7035554 0.0016886010 1.155040e-04 1.684723e-03 2 135783509 135785056 1548 + 2.847 2.931 0.278