ENSG00000143847

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367240 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding protein_coding 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.04046676 0.03354699 0.11887013 0.03354699 0.06449844 1.5652730 0.04742083 0.02676667 0.20806667 0.181300000 0.449998494 0.005866143 FALSE TRUE
ENST00000486360 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding retained_intron 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.09934864 0.16736709 0.05695346 0.08201952 0.01286509 -1.4055078 0.07647917 0.09696667 0.10756667 0.010600000 0.977596499 0.005866143 FALSE TRUE
ENST00000594572 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding processed_transcript 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.32216434 0.00000000 0.04514693 0.00000000 0.04514693 2.4632807 0.19898333 0.00000000 0.09966667 0.099666667 0.695925804 0.005866143 FALSE FALSE
ENST00000594656 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding retained_intron 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.06148722 0.07202861 0.03512256 0.03726659 0.03512256 -0.8622783 0.07142917 0.05433333 0.08260000 0.028266667 1.000000000 0.005866143   FALSE
ENST00000599966 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding protein_coding 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.05615027 0.01852660 0.07461521 0.01852660 0.04028126 1.5686090 0.07017917 0.01900000 0.14373333 0.124733333 0.486447214 0.005866143 FALSE TRUE
ENST00000601609 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding retained_intron 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.14230414 0.17315322 0.04985105 0.17315322 0.04985105 -1.6136027 0.09680833 0.06683333 0.07340000 0.006566667 0.968927281 0.005866143 FALSE FALSE
ENST00000688357 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding processed_transcript 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.16530299 0.38843246 0.00000000 0.03971646 0.00000000 -5.3162633 0.14873750 0.27223333 0.00000000 -0.272233333 0.005866143 0.005866143 FALSE TRUE
ENST00000692772 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding protein_coding 1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.04617712 0.00000000 0.04883921 0.00000000 0.04883921 2.5567779 0.05151667 0.00000000 0.11483333 0.114833333 0.728017239 0.005866143 TRUE TRUE
MSTRG.2870.9 ENSG00000143847 HEK293_OSMI2_6hA HEK293_TMG_6hB PPFIA4 protein_coding   1.27935 1.606247 0.5191646 0.4985253 0.08043966 -1.610859 0.17464457 0.50876581 0.03027282 0.16524331 0.03027282 -3.6872051 0.12278333 0.31313333 0.07120000 -0.241933333 0.165982785 0.005866143 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000143847 E001 0.0000000       1 203026491 203026497 7 +      
ENSG00000143847 E002 1.2544762 0.015378759 0.118776144   1 203026498 203026625 128 + 0.000 0.364 10.677
ENSG00000143847 E003 0.7788594 0.018364624 0.289447104   1 203026626 203026629 4 + 0.000 0.260 12.471
ENSG00000143847 E004 0.0000000       1 203034484 203034589 106 +      
ENSG00000143847 E005 0.0000000       1 203034703 203034780 78 +      
ENSG00000143847 E006 0.0000000       1 203035239 203035322 84 +      
ENSG00000143847 E007 0.0000000       1 203035559 203035640 82 +      
ENSG00000143847 E008 0.0000000       1 203037174 203037202 29 +      
ENSG00000143847 E009 4.7955440 0.006003627 0.621182832 0.78465905 1 203038610 203038946 337 + 0.735 0.665 -0.292
ENSG00000143847 E010 5.2424892 0.006044214 0.009969053 0.05803025 1 203038947 203039242 296 + 0.965 0.615 -1.398
ENSG00000143847 E011 3.1769178 0.009007519 0.259827518 0.49882642 1 203043397 203043498 102 + 0.681 0.497 -0.828
ENSG00000143847 E012 4.5407774 0.053318668 0.431955188 0.65271968 1 203043931 203044095 165 + 0.783 0.632 -0.627
ENSG00000143847 E013 4.1886837 0.112337389 0.827360497 0.91226086 1 203044379 203044453 75 + 0.621 0.637 0.068
ENSG00000143847 E014 3.3209575 0.008243905 0.621429313 0.78482446 1 203044696 203044785 90 + 0.620 0.540 -0.360
ENSG00000143847 E015 1.8684761 0.011873650 0.862452525 0.93168288 1 203045368 203045408 41 + 0.355 0.395 0.226
ENSG00000143847 E016 2.3558594 0.011193908 0.910360215 0.95733832 1 203045409 203045465 57 + 0.462 0.449 -0.068
ENSG00000143847 E017 2.7505569 0.074051509 0.365381629 0.60025693 1 203045466 203045559 94 + 0.620 0.454 -0.780
ENSG00000143847 E018 3.9930790 0.007247959 0.320541816 0.56019031 1 203045841 203045987 147 + 0.462 0.649 0.863
ENSG00000143847 E019 3.3475745 0.007619098 0.363859223 0.59892008 1 203046248 203046382 135 + 0.681 0.540 -0.625
ENSG00000143847 E020 0.6841482 0.020697718 0.187346556   1 203046383 203046507 125 + 0.356 0.124 -1.945
ENSG00000143847 E021 3.8008261 0.006700731 0.662559405 0.81148905 1 203048227 203048310 84 + 0.548 0.632 0.376
ENSG00000143847 E022 0.0000000       1 203048571 203048582 12 +      
ENSG00000143847 E023 3.9693289 0.036109965 0.701294050 0.83643544 1 203048583 203048714 132 + 0.683 0.612 -0.302
ENSG00000143847 E024 2.4253292 0.278485551 0.921989545 0.96327782 1 203048918 203048980 63 + 0.550 0.447 -0.501
ENSG00000143847 E025 2.0000843 0.057685454 0.231238321 0.46657303 1 203049676 203049767 92 + 0.213 0.470 1.622
ENSG00000143847 E026 0.6370325 0.020195400 0.807047751   1 203051183 203051239 57 + 0.213 0.174 -0.361
ENSG00000143847 E027 3.5920026 0.008233134 0.201837749 0.43193029 1 203051240 203051768 529 + 0.735 0.540 -0.846
ENSG00000143847 E028 4.0835090 0.007939127 0.179791885 0.40368875 1 203051769 203051877 109 + 0.783 0.579 -0.859
ENSG00000143847 E029 0.3336024 0.028913282 1.000000000   1 203053703 203053745 43 + 0.000 0.123 11.367
ENSG00000143847 E030 0.3336024 0.028913282 1.000000000   1 203053746 203053752 7 + 0.000 0.123 11.367
ENSG00000143847 E031 1.8102141 0.017260219 0.311835879 0.55220922 1 203053753 203053795 43 + 0.213 0.422 1.377
ENSG00000143847 E032 1.9522285 0.010577409 0.260259802 0.49921994 1 203053796 203053860 65 + 0.213 0.448 1.514
ENSG00000143847 E033 4.1632404 0.007308968 0.279533391 0.51964007 1 203053861 203053961 101 + 0.462 0.665 0.931
ENSG00000143847 E034 0.0000000       1 203054058 203054123 66 +      
ENSG00000143847 E035 6.6416160 0.005436595 0.863331258 0.93221112 1 203055432 203055672 241 + 0.783 0.818 0.139
ENSG00000143847 E036 3.1224750 0.008053213 0.986178898 0.99491397 1 203056120 203056155 36 + 0.548 0.559 0.053
ENSG00000143847 E037 3.0349506 0.008208189 0.257401098 0.49614227 1 203056375 203056451 77 + 0.355 0.579 1.142
ENSG00000143847 E038 3.5876105 0.007835142 0.429019125 0.65033782 1 203056452 203056508 57 + 0.462 0.615 0.717
ENSG00000143847 E039 2.6056416 0.009314485 0.115400683 0.30814564 1 203056784 203056950 167 + 0.213 0.540 1.961
ENSG00000143847 E040 3.0757167 0.042437957 0.738658793 0.85966056 1 203059178 203059271 94 + 0.462 0.544 0.394
ENSG00000143847 E041 3.4560773 0.007404947 0.810902794 0.90278516 1 203059770 203059851 82 + 0.548 0.597 0.223
ENSG00000143847 E042 7.7686773 0.004083602 0.970121125 0.98690526 1 203060217 203060417 201 + 0.865 0.869 0.017
ENSG00000143847 E043 4.7590973 0.005937592 0.401733623 0.62980484 1 203060970 203061032 63 + 0.783 0.664 -0.486
ENSG00000143847 E044 0.9172423 0.149728366 0.398842884   1 203061366 203061651 286 + 0.357 0.178 -1.335
ENSG00000143847 E045 0.6839606 0.019804580 0.816860424   1 203061652 203061678 27 + 0.213 0.174 -0.360
ENSG00000143847 E046 1.0239076 0.019019400 0.457570649   1 203062959 203063827 869 + 0.355 0.220 -0.940
ENSG00000143847 E047 8.0827586 0.004192059 0.753504544 0.86883170 1 203063828 203064003 176 + 0.901 0.870 -0.119
ENSG00000143847 E048 0.3751086 0.025485084 1.000000000   1 203067445 203067694 250 + 0.000 0.124 11.373
ENSG00000143847 E049 5.4842514 0.006255163 0.990394748 0.99692850 1 203067695 203067792 98 + 0.735 0.748 0.051
ENSG00000143847 E050 7.3822973 0.219446860 0.819604310 0.90787194 1 203068453 203068628 176 + 0.828 0.852 0.093
ENSG00000143847 E051 5.7372419 0.169081521 0.828062812 0.91263864 1 203071692 203071760 69 + 0.784 0.748 -0.146
ENSG00000143847 E052 5.8695108 0.005881304 0.132963260 0.33646407 1 203075577 203075757 181 + 0.548 0.807 1.097
ENSG00000143847 E053 0.6894974 0.478954802 0.716260369   1 203075758 203075967 210 + 0.000 0.225 11.977
ENSG00000143847 E054 0.8203656 0.035376838 0.301468468   1 203075968 203076002 35 + 0.000 0.261 12.460
ENSG00000143847 E055 1.6415390 0.026594433 0.978684114 0.99112697 1 203076003 203076076 74 + 0.355 0.365 0.060
ENSG00000143847 E056 3.5625526 0.015701578 0.695121444 0.83235185 1 203076077 203076340 264 + 0.620 0.561 -0.263
ENSG00000143847 E057 5.2255906 0.008954063 0.266205748 0.50571659 1 203076341 203076479 139 + 0.826 0.665 -0.654
ENSG00000143847 E058 56.3293453 0.001516763 0.243299364 0.48035049 1 203076480 203078740 2261 + 1.612 1.683 0.241