ENSG00000143753

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323699 ENSG00000143753 HEK293_OSMI2_6hA HEK293_TMG_6hB DEGS1 protein_coding protein_coding 126.5029 51.91368 151.1206 2.684887 2.884443 1.541331 104.297393 43.539876 117.04875 2.735705 3.5544859 1.426492 0.83578750 0.8384667 0.7745667 -0.06390000 5.802405e-01 1.343916e-36 FALSE TRUE
ENST00000415210 ENSG00000143753 HEK293_OSMI2_6hA HEK293_TMG_6hB DEGS1 protein_coding protein_coding 126.5029 51.91368 151.1206 2.684887 2.884443 1.541331 4.265968 0.000000 16.38002 0.000000 0.8068664 10.678602 0.02613333 0.0000000 0.1084000 0.10840000 1.343916e-36 1.343916e-36 FALSE TRUE
MSTRG.3113.4 ENSG00000143753 HEK293_OSMI2_6hA HEK293_TMG_6hB DEGS1 protein_coding   126.5029 51.91368 151.1206 2.684887 2.884443 1.541331 15.254650 6.393083 15.60488 1.141467 2.3743231 1.286082 0.11532083 0.1231000 0.1029667 -0.02013333 8.926921e-01 1.343916e-36 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000143753 E001 0.1779838 0.0375535850 1.000000e+00   1 224165879 224165919 41 + 0.086 0.000 -8.302
ENSG00000143753 E002 0.2735028 0.0267240366 5.791765e-01   1 224175756 224175947 192 + 0.086 0.169 1.115
ENSG00000143753 E003 184.7649740 1.4800440685 3.787714e-01 0.611224812 1 224183240 224183265 26 + 2.107 2.432 1.086
ENSG00000143753 E004 394.6814253 1.7546410382 4.728994e-01 0.682089258 1 224183266 224183418 153 + 2.469 2.724 0.849
ENSG00000143753 E005 1.9462821 0.0099815129 4.849992e-01 0.690564800 1 224183675 224183831 157 + 0.404 0.528 0.631
ENSG00000143753 E006 1862.0517448 0.0005020022 2.185776e-01 0.451734839 1 224189577 224190135 559 + 3.241 3.237 -0.011
ENSG00000143753 E007 1249.2630426 0.0047118728 1.209036e-02 0.066820576 1 224190136 224190319 184 + 3.086 3.024 -0.206
ENSG00000143753 E008 24.1246766 0.0120088003 2.319764e-01 0.467456952 1 224191092 224191156 65 + 1.326 1.443 0.403
ENSG00000143753 E009 1205.7279565 0.0027491607 4.637085e-03 0.032671603 1 224192332 224192518 187 + 3.068 3.013 -0.184
ENSG00000143753 E010 1130.7098160 0.0017084139 8.723328e-01 0.937217656 1 224192519 224192822 304 + 3.021 3.029 0.028
ENSG00000143753 E011 393.9768086 0.0001682821 6.848552e-01 0.825856491 1 224192823 224192895 73 + 2.563 2.577 0.049
ENSG00000143753 E012 579.9123021 0.0012010093 1.245997e-01 0.323106106 1 224192896 224193441 546 + 2.720 2.763 0.143
ENSG00000143753 E013 7.9509019 0.0729547457 1.647033e-05 0.000323844 1 224200963 224201163 201 + 0.555 1.274 2.779