ENSG00000143494

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366965 ENSG00000143494 HEK293_OSMI2_6hA HEK293_TMG_6hB VASH2 protein_coding protein_coding 2.197269 1.591893 3.031822 0.3828721 0.2988117 0.9251579 0.8840358 0.4818340 1.33303302 0.10636098 0.20411352 1.449251 0.37371250 0.3195667 0.4425333 0.1229667 0.72236439 0.04995301 FALSE TRUE
ENST00000366967 ENSG00000143494 HEK293_OSMI2_6hA HEK293_TMG_6hB VASH2 protein_coding protein_coding 2.197269 1.591893 3.031822 0.3828721 0.2988117 0.9251579 0.2445926 0.0000000 0.56898526 0.00000000 0.28467590 5.855455 0.07939167 0.0000000 0.1745000 0.1745000 0.35945103 0.04995301 FALSE TRUE
ENST00000366968 ENSG00000143494 HEK293_OSMI2_6hA HEK293_TMG_6hB VASH2 protein_coding protein_coding 2.197269 1.591893 3.031822 0.3828721 0.2988117 0.9251579 0.3236943 0.1810470 0.63259295 0.09114285 0.14427173 1.749978 0.18835417 0.1514333 0.2181333 0.0667000 0.85050973 0.04995301 FALSE TRUE
ENST00000493155 ENSG00000143494 HEK293_OSMI2_6hA HEK293_TMG_6hB VASH2 protein_coding processed_transcript 2.197269 1.591893 3.031822 0.3828721 0.2988117 0.9251579 0.2050006 0.4860699 0.07300615 0.12507900 0.07300615 -2.579253 0.11647917 0.3034000 0.0288000 -0.2746000 0.04995301 0.04995301 FALSE FALSE
ENST00000517399 ENSG00000143494 HEK293_OSMI2_6hA HEK293_TMG_6hB VASH2 protein_coding protein_coding 2.197269 1.591893 3.031822 0.3828721 0.2988117 0.9251579 0.2574117 0.2175240 0.09844849 0.18099820 0.05077326 -1.069009 0.10955833 0.1050000 0.0363000 -0.0687000 0.84052666 0.04995301 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000143494 E001 0.0000000       1 212950520 212950540 21 +      
ENSG00000143494 E002 0.4929928 0.022928195 1.000000e+00   1 212950541 212950593 53 + 0.169 0.158 -0.109
ENSG00000143494 E003 0.4929928 0.022928195 1.000000e+00   1 212950594 212950594 1 + 0.168 0.158 -0.109
ENSG00000143494 E004 1.6172211 0.042142269 3.457972e-02 0.1404450999 1 212950595 212950617 23 + 0.233 0.611 2.117
ENSG00000143494 E005 1.9322301 0.148004188 7.385140e-02 0.2324812599 1 212950618 212950634 17 + 0.288 0.657 1.913
ENSG00000143494 E006 2.0936458 0.076501257 8.928728e-02 0.2624396137 1 212950635 212950640 6 + 0.338 0.656 1.581
ENSG00000143494 E007 2.6467382 0.080199968 5.707622e-02 0.1962136902 1 212950641 212950666 26 + 0.384 0.736 1.646
ENSG00000143494 E008 3.2957976 0.012564630 3.806887e-01 0.6127643142 1 212950667 212950740 74 + 0.557 0.696 0.603
ENSG00000143494 E009 0.0000000       1 212951182 212951338 157 +      
ENSG00000143494 E010 1.6480289 0.011551772 7.237051e-01 0.8503605944 1 212951339 212951392 54 + 0.425 0.365 -0.332
ENSG00000143494 E011 1.1246762 0.014572110 7.027913e-01   1 212951393 212951396 4 + 0.340 0.274 -0.434
ENSG00000143494 E012 1.5703657 0.011527787 3.548820e-01 0.5916430431 1 212951397 212951527 131 + 0.339 0.504 0.888
ENSG00000143494 E013 1.8754458 0.040256233 1.041202e-02 0.0599064866 1 212951528 212951588 61 + 0.233 0.692 2.465
ENSG00000143494 E014 3.0282690 0.108063799 1.466216e-01 0.3573705674 1 212951589 212951634 46 + 0.463 0.761 1.324
ENSG00000143494 E015 10.5145580 0.041482318 3.955910e-01 0.6248820621 1 212951635 212951818 184 + 1.002 1.128 0.459
ENSG00000143494 E016 4.8676026 0.006018825 5.990478e-06 0.0001346553 1 212952430 212952791 362 + 0.425 1.045 2.604
ENSG00000143494 E017 9.8033260 0.003709532 5.975256e-01 0.7686423663 1 212961166 212961226 61 + 1.040 0.990 -0.183
ENSG00000143494 E018 7.9963521 0.005495875 4.746033e-01 0.6832760303 1 212961227 212961254 28 + 0.979 0.903 -0.284
ENSG00000143494 E019 4.3439985 0.007054869 6.939069e-01 0.8316139143 1 212961255 212961360 106 + 0.702 0.765 0.257
ENSG00000143494 E020 5.1784366 0.020371426 4.808887e-01 0.6876959960 1 212965722 212965778 57 + 0.824 0.729 -0.377
ENSG00000143494 E021 1.0382672 0.855698091 8.002930e-01   1 212965779 212966155 377 + 0.291 0.342 0.326
ENSG00000143494 E022 8.4208122 0.004054760 3.758887e-02 0.1485512840 1 212966271 212966345 75 + 1.039 0.797 -0.918
ENSG00000143494 E023 0.0000000       1 212966957 212967096 140 +      
ENSG00000143494 E024 3.6018731 0.007808767 3.350313e-01 0.5736004121 1 212967114 212969249 2136 + 0.585 0.732 0.629
ENSG00000143494 E025 22.0804993 0.010190936 3.546225e-02 0.1427864989 1 212972580 212972961 382 + 1.408 1.234 -0.609
ENSG00000143494 E026 0.4929928 0.022928195 1.000000e+00   1 212973424 212973954 531 + 0.168 0.158 -0.109
ENSG00000143494 E027 10.8857671 0.022147403 3.098333e-02 0.1302944974 1 212973955 212974070 116 + 1.140 0.877 -0.968
ENSG00000143494 E028 0.0000000       1 212974597 212974927 331 +      
ENSG00000143494 E029 31.7348351 0.004855493 2.015997e-01 0.4316438893 1 212988512 212989126 615 + 1.530 1.449 -0.277
ENSG00000143494 E030 10.0853703 0.006539328 6.778624e-01 0.8213756733 1 212989127 212989176 50 + 1.049 1.009 -0.145
ENSG00000143494 E031 104.7804304 0.014985414 8.554363e-01 0.9278632126 1 212989177 212991550 2374 + 2.004 2.007 0.009
ENSG00000143494 E032 1.4264435 0.175075320 7.026681e-01 0.8372628075 1 212991551 212992037 487 + 0.391 0.277 -0.708