ENSG00000141232

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000499247 ENSG00000141232 HEK293_OSMI2_6hA HEK293_TMG_6hB TOB1 protein_coding protein_coding 14.01618 8.133294 22.43711 1.228285 1.960638 1.462846 11.184394 8.120907 17.342396 1.219589 0.9732854 1.093647 0.8634583 0.9987333 0.7790000 -0.2197333 7.175938e-11 7.175938e-11 FALSE TRUE
ENST00000509385 ENSG00000141232 HEK293_OSMI2_6hA HEK293_TMG_6hB TOB1 protein_coding processed_transcript 14.01618 8.133294 22.43711 1.228285 1.960638 1.462846 2.661386 0.000000 4.577542 0.000000 1.5526773 8.841578 0.1270583 0.0000000 0.1953667 0.1953667 3.673509e-08 7.175938e-11   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000141232 E001 149.2855526 0.038714941 0.008816577 0.05300423 17 50862223 50862979 757 - 2.086 2.269 0.612
ENSG00000141232 E002 197.2870299 0.187368939 0.381064241 0.61305500 17 50862980 50863368 389 - 2.295 2.288 -0.024
ENSG00000141232 E003 280.7491755 0.415581342 0.586201453 0.76107418 17 50863369 50863960 592 - 2.478 2.393 -0.283
ENSG00000141232 E004 37.4172636 0.001358150 0.181403785 0.40585646 17 50863961 50863966 6 - 1.635 1.541 -0.322
ENSG00000141232 E005 62.7527763 0.003295592 0.834846953 0.91643331 17 50863967 50864163 197 - 1.818 1.813 -0.015
ENSG00000141232 E006 58.8348167 1.767783492 0.603665307 0.77259913 17 50866058 50866354 297 - 1.785 1.752 -0.114
ENSG00000141232 E007 0.2852693 0.284349352 0.357497842   17 50867494 50867602 109 - 0.186 0.000 -9.666
ENSG00000141232 E008 0.1308682 0.707128996 0.693253839   17 50867603 50867804 202 - 0.000 0.136 9.601
ENSG00000141232 E009 0.4161375 0.084451600 0.883316509   17 50867805 50868281 477 - 0.187 0.140 -0.499