ENSG00000140350

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000267918 ENSG00000140350 HEK293_OSMI2_6hA HEK293_TMG_6hB ANP32A protein_coding processed_transcript 203.2915 181.2297 202.1819 31.86119 9.791104 0.1578263 41.08870 41.58686 43.83350 6.564593 3.0298552 0.07588819 0.20620000 0.23140000 0.21646667 -0.01493333 0.83465107 0.00233161   FALSE
ENST00000409628 ENSG00000140350 HEK293_OSMI2_6hA HEK293_TMG_6hB ANP32A protein_coding nonsense_mediated_decay 203.2915 181.2297 202.1819 31.86119 9.791104 0.1578263 15.02515 10.91864 12.75148 3.704133 2.5200272 0.22368132 0.07572917 0.07343333 0.06413333 -0.00930000 0.99293230 0.00233161   FALSE
ENST00000465139 ENSG00000140350 HEK293_OSMI2_6hA HEK293_TMG_6hB ANP32A protein_coding protein_coding 203.2915 181.2297 202.1819 31.86119 9.791104 0.1578263 51.47827 30.41755 55.31648 7.100658 2.3706072 0.86259190 0.24975417 0.16356667 0.27376667 0.11020000 0.00233161 0.00233161 FALSE TRUE
ENST00000560303 ENSG00000140350 HEK293_OSMI2_6hA HEK293_TMG_6hB ANP32A protein_coding protein_coding 203.2915 181.2297 202.1819 31.86119 9.791104 0.1578263 46.89427 51.31757 40.16336 12.022771 4.2046281 -0.35349488 0.22617500 0.27606667 0.19756667 -0.07850000 0.29722663 0.00233161 FALSE TRUE
MSTRG.11115.2 ENSG00000140350 HEK293_OSMI2_6hA HEK293_TMG_6hB ANP32A protein_coding   203.2915 181.2297 202.1819 31.86119 9.791104 0.1578263 36.43998 35.34358 34.80705 11.229250 0.8192143 -0.02206252 0.17892083 0.18560000 0.17293333 -0.01266667 0.98674600 0.00233161 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000140350 E001 201.116921 7.849343e-04 2.847708e-09 1.509968e-07 15 68778535 68778906 372 - 2.378 2.218 -0.536
ENSG00000140350 E002 347.862049 1.439200e-04 4.666039e-04 5.370024e-03 15 68778907 68778993 87 - 2.572 2.511 -0.206
ENSG00000140350 E003 945.404736 9.929917e-05 6.171660e-03 4.056703e-02 15 68778994 68779323 330 - 2.989 2.962 -0.089
ENSG00000140350 E004 1511.862277 6.693336e-04 7.944810e-06 1.719179e-04 15 68779324 68779742 419 - 3.207 3.149 -0.194
ENSG00000140350 E005 663.146341 2.015665e-03 1.993922e-01 4.289727e-01 15 68779743 68779915 173 - 2.833 2.808 -0.083
ENSG00000140350 E006 523.903382 9.604192e-04 3.817758e-05 6.623856e-04 15 68779916 68780071 156 - 2.671 2.765 0.312
ENSG00000140350 E007 896.484904 1.104555e-04 5.078141e-01 7.062807e-01 15 68780072 68780139 68 - 2.947 2.961 0.047
ENSG00000140350 E008 658.273038 1.968313e-04 1.395981e-01 3.468821e-01 15 68780140 68780142 3 - 2.828 2.811 -0.055
ENSG00000140350 E009 742.166379 8.849088e-04 1.924582e-01 4.200359e-01 15 68780410 68780419 10 - 2.882 2.861 -0.071
ENSG00000140350 E010 911.912028 2.624020e-03 4.483814e-01 6.645311e-01 15 68780420 68780470 51 - 2.970 2.951 -0.064
ENSG00000140350 E011 518.432485 5.980734e-03 2.296425e-01 4.647179e-01 15 68780471 68780473 3 - 2.740 2.690 -0.164
ENSG00000140350 E012 49.250727 6.293829e-02 4.162811e-01 6.408298e-01 15 68780474 68781605 1132 - 1.638 1.756 0.400
ENSG00000140350 E013 10.353370 1.582441e-01 8.675700e-01 9.346040e-01 15 68782772 68782955 184 - 1.026 1.075 0.179
ENSG00000140350 E014 1484.830634 4.868398e-04 1.234787e-01 3.212515e-01 15 68782956 68783053 98 - 3.180 3.164 -0.055
ENSG00000140350 E015 4.364728 9.504246e-02 6.241426e-01 7.867220e-01 15 68784271 68784384 114 - 0.683 0.772 0.366
ENSG00000140350 E016 5.397527 1.784621e-01 8.727291e-01 9.374295e-01 15 68784385 68784396 12 - 0.810 0.796 -0.059
ENSG00000140350 E017 2243.419239 5.430563e-05 9.282594e-01 9.661417e-01 15 68784397 68784595 199 - 3.348 3.355 0.023
ENSG00000140350 E018 3.531306 3.628785e-01 6.714280e-01 8.172000e-01 15 68787179 68787412 234 - 0.680 0.634 -0.196
ENSG00000140350 E019 1429.522852 1.365596e-03 2.866977e-02 1.233885e-01 15 68787413 68787495 83 - 3.129 3.180 0.169
ENSG00000140350 E020 1032.510887 1.239163e-03 8.362380e-02 2.520409e-01 15 68787496 68787535 40 - 2.993 3.035 0.142
ENSG00000140350 E021 1551.263509 1.332053e-03 2.065242e-02 9.818614e-02 15 68787770 68787919 150 - 3.164 3.217 0.176
ENSG00000140350 E022 45.670490 1.038768e-01 3.588407e-04 4.325717e-03 15 68789243 68792248 3006 - 1.249 1.880 2.160
ENSG00000140350 E023 0.333790 3.784859e-02 9.938290e-01   15 68812946 68813034 89 - 0.120 0.123 0.046
ENSG00000140350 E024 2.957070 7.863224e-02 8.440071e-02 2.535186e-01 15 68817385 68817549 165 - 0.412 0.761 1.591
ENSG00000140350 E025 1.493180 1.292554e-02 1.338730e-02 7.197888e-02 15 68817550 68817715 166 - 0.120 0.559 3.043
ENSG00000140350 E026 956.481449 1.276480e-03 3.254177e-03 2.492824e-02 15 68820698 68820977 280 - 2.946 3.015 0.227