ENSG00000140323

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000267889 ENSG00000140323 HEK293_OSMI2_6hA HEK293_TMG_6hB DISP2 protein_coding protein_coding 1.011558 2.643694 0.2551422 0.2149072 0.1141476 -3.323163 0.2983809 0.5846543 0.1249910 0.02258574 0.01868969 -2.1391875 0.4803625 0.22300000 0.7303667 0.5073667 4.123546e-01 1.579462e-05 FALSE TRUE
ENST00000558623 ENSG00000140323 HEK293_OSMI2_6hA HEK293_TMG_6hB DISP2 protein_coding processed_transcript 1.011558 2.643694 0.2551422 0.2149072 0.1141476 -3.323163 0.5818679 1.8121833 0.0000000 0.18476513 0.00000000 -7.5095243 0.3408958 0.68613333 0.0000000 -0.6861333 1.579462e-05 1.579462e-05   FALSE
ENST00000559721 ENSG00000140323 HEK293_OSMI2_6hA HEK293_TMG_6hB DISP2 protein_coding retained_intron 1.011558 2.643694 0.2551422 0.2149072 0.1141476 -3.323163 0.1233457 0.2468560 0.1301512 0.15822290 0.13015120 -0.8739757 0.1617208 0.09086667 0.2696333 0.1787667 9.418345e-01 1.579462e-05   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000140323 E001 3.7546202 0.007304877 1.536402e-01 3.675030e-01 15 40358219 40358440 222 + 0.262 0.576 1.742
ENSG00000140323 E002 11.6311902 0.003138024 8.658930e-01 9.336889e-01 15 40363625 40363954 330 + 0.967 0.944 -0.087
ENSG00000140323 E003 4.9092810 0.005608387 9.848140e-01 9.942373e-01 15 40364226 40364255 30 + 0.635 0.630 -0.018
ENSG00000140323 E004 7.8349439 0.004359648 6.663195e-02 2.174788e-01 15 40364421 40364544 124 + 1.004 0.752 -0.968
ENSG00000140323 E005 7.2035656 0.009657668 1.710446e-01 3.919213e-01 15 40364838 40364953 116 + 0.927 0.723 -0.798
ENSG00000140323 E006 0.6368449 0.019268599 8.303304e-01   15 40365007 40365146 140 + 0.000 0.177 10.316
ENSG00000140323 E007 8.2948686 0.079648739 8.645543e-01 9.329149e-01 15 40365147 40365274 128 + 0.772 0.813 0.162
ENSG00000140323 E008 7.6161576 0.058473168 4.136053e-01 6.387106e-01 15 40365628 40365694 67 + 0.882 0.754 -0.501
ENSG00000140323 E009 4.2333126 0.016904889 9.175581e-01 9.610045e-01 15 40365695 40365725 31 + 0.542 0.564 0.099
ENSG00000140323 E010 67.4521385 0.010878677 1.676416e-02 8.460943e-02 15 40367058 40369854 2797 + 1.521 1.683 0.553
ENSG00000140323 E011 8.3178150 0.019088812 7.322748e-01 8.556078e-01 15 40369855 40369928 74 + 0.776 0.836 0.235
ENSG00000140323 E012 29.9080345 0.013077615 9.201042e-01 9.622553e-01 15 40369929 40370611 683 + 1.318 1.329 0.039
ENSG00000140323 E013 17.7641309 0.002683940 9.012378e-02 2.640174e-01 15 40370612 40371056 445 + 0.927 1.136 0.768
ENSG00000140323 E014 0.4644705 0.023950367 1.000000e+00   15 40371057 40371824 768 + 0.000 0.139 9.950
ENSG00000140323 E015 0.5177432 0.024441170 3.384029e-01   15 40371825 40372122 298 + 0.262 0.098 -1.714
ENSG00000140323 E016 0.3040503 0.027442404 7.136421e-03   15 40372123 40372373 251 + 0.424 0.000 -13.996
ENSG00000140323 E017 0.1614157 0.048288224 5.219433e-02   15 40372374 40372476 103 + 0.262 0.000 -12.983
ENSG00000140323 E018 4.4320526 0.006832223 2.258359e-08 9.793701e-07 15 40372477 40378621 6145 + 1.154 0.377 -3.258