ENSG00000138777

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000351450 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding nonsense_mediated_decay 87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 11.071011 3.747845 20.236898 2.0564789 1.6742143 2.4297234 0.12350000 0.10906667 0.14396667 0.03490000 8.072461e-01 1.791697e-05 FALSE TRUE
ENST00000457404 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding processed_transcript 87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 12.228399 0.613646 25.264166 0.6136460 1.3947435 5.3407924 0.10115833 0.02980000 0.18020000 0.15040000 1.532829e-01 1.791697e-05 FALSE FALSE
ENST00000499847 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding retained_intron 87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 8.330839 4.001708 9.422932 1.0016679 0.8066299 1.2334897 0.12370833 0.13563333 0.06693333 -0.06870000 2.277916e-01 1.791697e-05 FALSE TRUE
ENST00000502596 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding protein_coding 87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 3.392325 2.811047 3.597659 0.7007323 0.4633759 0.3548322 0.04686667 0.09050000 0.02566667 -0.06483333 4.091211e-05 1.791697e-05 TRUE FALSE
ENST00000505713 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding processed_transcript 87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 3.604974 3.061274 4.515857 0.1917435 0.3662560 0.5593534 0.05195833 0.10606667 0.03230000 -0.07376667 1.791697e-05 1.791697e-05 FALSE FALSE
MSTRG.25266.10 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding   87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 24.254200 7.736669 39.435468 1.7366598 2.7348643 2.3482116 0.26752500 0.26306667 0.28116667 0.01810000 9.527941e-01 1.791697e-05 FALSE TRUE
MSTRG.25266.19 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding   87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 6.207643 2.553637 9.170925 0.4715518 0.3741111 1.8404476 0.07416667 0.08440000 0.06533333 -0.01906667 4.644650e-01 1.791697e-05 FALSE TRUE
MSTRG.25266.3 ENSG00000138777 HEK293_OSMI2_6hA HEK293_TMG_6hB PPA2 protein_coding   87.14239 29.97013 140.3759 4.749147 4.343302 2.22732 5.939444 1.885586 10.184219 0.3641483 2.0927309 2.4270351 0.06969583 0.06416667 0.07176667 0.00760000 9.607057e-01 1.791697e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000138777 E001 2.5318452 0.0088706742 5.631071e-01 7.454963e-01 4 105369077 105369242 166 - 0.457 0.566 0.525
ENSG00000138777 E002 7.5033938 0.0157759501 3.923004e-02 1.527739e-01 4 105369243 105369520 278 - 0.764 1.046 1.073
ENSG00000138777 E003 3.6401939 0.1401022947 1.425761e-01 3.513006e-01 4 105369521 105369560 40 - 0.499 0.814 1.354
ENSG00000138777 E004 2.5934885 0.0267065800 9.835949e-02 2.789110e-01 4 105369561 105369564 4 - 0.406 0.702 1.380
ENSG00000138777 E005 8.1882520 0.0045073301 7.076667e-01 8.403415e-01 4 105369565 105369579 15 - 0.867 0.923 0.212
ENSG00000138777 E006 348.2177052 0.0016271526 9.993529e-01 1.000000e+00 4 105369580 105369753 174 - 2.440 2.446 0.020
ENSG00000138777 E007 269.8664215 0.0027715545 4.532858e-01 6.681859e-01 4 105370837 105370873 37 - 2.336 2.314 -0.072
ENSG00000138777 E008 287.7752974 0.0019484829 8.418932e-01 9.205217e-01 4 105386567 105386612 46 - 2.357 2.372 0.049
ENSG00000138777 E009 218.6148925 0.0020125961 9.945893e-01 9.988905e-01 4 105386613 105386636 24 - 2.240 2.249 0.030
ENSG00000138777 E010 337.2867944 0.0018106204 4.344365e-01 6.545741e-01 4 105396249 105396325 77 - 2.422 2.455 0.109
ENSG00000138777 E011 204.2168553 0.0013603496 1.280509e-01 3.286877e-01 4 105396326 105396334 9 - 2.200 2.260 0.202
ENSG00000138777 E012 9.0460234 0.0172024735 7.454015e-01 8.639369e-01 4 105398304 105398867 564 - 0.904 0.954 0.190
ENSG00000138777 E013 311.8892051 0.0010038975 7.739202e-01 8.810946e-01 4 105399037 105399078 42 - 2.395 2.394 -0.003
ENSG00000138777 E014 366.4684727 0.0001521091 3.221700e-01 5.615966e-01 4 105399079 105399150 72 - 2.466 2.449 -0.056
ENSG00000138777 E015 227.8293566 0.0002113974 3.631904e-01 5.982819e-01 4 105399151 105399164 14 - 2.261 2.240 -0.069
ENSG00000138777 E016 7.8666947 0.1468050083 2.112647e-01 4.432904e-01 4 105399165 105399636 472 - 0.789 1.052 0.994
ENSG00000138777 E017 11.0907253 0.0032287721 9.716605e-01 9.877130e-01 4 105405637 105405837 201 - 0.987 0.987 0.002
ENSG00000138777 E018 9.7882529 0.0051308935 6.132965e-01 7.791491e-01 4 105405838 105405948 111 - 0.950 0.887 -0.240
ENSG00000138777 E019 2.0082552 0.2812130938 4.571860e-01 6.711704e-01 4 105422155 105422155 1 - 0.449 0.226 -1.404
ENSG00000138777 E020 15.8890527 0.0025502877 5.653358e-03 3.801180e-02 4 105422156 105422433 278 - 1.184 0.847 -1.243
ENSG00000138777 E021 8.4511857 0.0215803813 3.323994e-01 5.711742e-01 4 105423204 105423381 178 - 0.904 0.756 -0.578
ENSG00000138777 E022 372.6658013 0.0002506260 4.936726e-02 1.780696e-01 4 105424196 105424248 53 - 2.477 2.436 -0.135
ENSG00000138777 E023 405.4004140 0.0001792346 1.002769e-03 9.936078e-03 4 105424249 105424322 74 - 2.518 2.449 -0.231
ENSG00000138777 E024 256.6738980 0.0001971362 1.808752e-02 8.932151e-02 4 105437950 105437976 27 - 2.320 2.258 -0.205
ENSG00000138777 E025 339.9707919 0.0002444021 1.683480e-02 8.485570e-02 4 105437977 105438014 38 - 2.439 2.385 -0.179
ENSG00000138777 E026 296.5882877 0.0002221626 3.965613e-02 1.538581e-01 4 105438015 105438036 22 - 2.379 2.331 -0.162
ENSG00000138777 E027 20.4667842 0.0028418392 3.650678e-03 2.719998e-02 4 105446202 105446382 181 - 1.166 1.423 0.901
ENSG00000138777 E028 461.9497229 0.0001692359 1.583890e-02 8.121649e-02 4 105446383 105446502 120 - 2.571 2.526 -0.150
ENSG00000138777 E029 0.1426347 0.0324437409 1.000000e+00   4 105447995 105448093 99 - 0.063 0.000 -7.939
ENSG00000138777 E030 297.6396852 0.0009377201 1.850657e-02 9.074440e-02 4 105449350 105449403 54 - 2.384 2.318 -0.218
ENSG00000138777 E031 303.1570801 0.0003877792 9.773088e-02 2.777889e-01 4 105453598 105453642 45 - 2.388 2.348 -0.132
ENSG00000138777 E032 0.1614157 0.0341117432 1.000000e+00   4 105453781 105453908 128 - 0.063 0.000 -7.938
ENSG00000138777 E033 0.4764247 0.0215782394 5.301371e-01   4 105456081 105456247 167 - 0.117 0.223 1.111
ENSG00000138777 E034 2.1793916 0.0145376552 7.386184e-01 8.596402e-01 4 105456248 105456291 44 - 0.433 0.370 -0.350
ENSG00000138777 E035 1.5961116 0.0117559511 4.093768e-01 6.355752e-01 4 105456292 105456680 389 - 0.319 0.479 0.889
ENSG00000138777 E036 232.6970099 0.0002352994 8.473075e-02 2.542031e-01 4 105456681 105456695 15 - 2.274 2.228 -0.154
ENSG00000138777 E037 267.4334280 0.0002438952 6.443371e-01 7.996789e-01 4 105456696 105456745 50 - 2.327 2.320 -0.024
ENSG00000138777 E038 87.4668901 0.0029110381 6.342385e-13 6.744414e-11 4 105473145 105473339 195 - 1.751 2.099 1.171
ENSG00000138777 E039 35.3301216 0.0012629132 5.348801e-12 4.844531e-10 4 105473340 105473357 18 - 1.329 1.773 1.518
ENSG00000138777 E040 30.8156601 0.0104107741 6.789258e-08 2.624567e-06 4 105473358 105473378 21 - 1.274 1.711 1.503
ENSG00000138777 E041 82.3940961 0.0005305859 1.165973e-36 1.596846e-33 4 105473379 105473775 397 - 1.656 2.183 1.774
ENSG00000138777 E042 248.5431567 0.0120914810 7.706810e-02 2.389307e-01 4 105473894 105474076 183 - 2.273 2.371 0.329