ENSG00000138071

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000260641 ENSG00000138071 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTR2 protein_coding protein_coding 84.72864 28.81685 143.6134 7.281171 5.529693 2.316806 63.96924 25.422827 105.8394 6.8360975 4.004271 2.057249 0.7972667 0.8710000 0.7370 -0.1340000 0.0135857390 0.0005874431 FALSE TRUE
MSTRG.18542.1 ENSG00000138071 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTR2 protein_coding   84.72864 28.81685 143.6134 7.281171 5.529693 2.316806 20.65959 3.300071 37.5891 0.5834086 1.650737 3.505764 0.2011167 0.1224333 0.2616 0.1391667 0.0005874431 0.0005874431 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000138071 E001 0.4820342 0.0213444008 7.207876e-01   2 65227700 65227702 3 + 0.158 0.000 -8.578
ENSG00000138071 E002 6.0456984 0.0044192002 6.471383e-01 8.015563e-01 2 65227703 65227787 85 + 0.754 0.723 -0.126
ENSG00000138071 E003 73.4195386 0.0046797921 2.087822e-01 4.404036e-01 2 65227788 65227830 43 + 1.762 1.729 -0.113
ENSG00000138071 E004 73.2816559 0.0053801711 1.622706e-01 3.801685e-01 2 65227831 65227832 2 + 1.763 1.717 -0.155
ENSG00000138071 E005 73.6210555 0.0048614895 1.438836e-01 3.532214e-01 2 65227833 65227836 4 + 1.766 1.718 -0.162
ENSG00000138071 E006 387.5662131 0.0029077797 3.358180e-02 1.377095e-01 2 65227837 65227957 121 + 2.475 2.448 -0.092
ENSG00000138071 E007 617.9590968 0.0002833567 2.131823e-09 1.167094e-07 2 65239852 65239962 111 + 2.685 2.614 -0.235
ENSG00000138071 E008 0.4929928 0.0232651104 5.164468e-01   2 65241972 65242048 77 + 0.111 0.234 1.291
ENSG00000138071 E009 0.3150090 0.0470256509 2.773762e-01   2 65242049 65242063 15 + 0.059 0.234 2.294
ENSG00000138071 E010 0.5173834 0.0610440549 7.212556e-01   2 65246185 65246307 123 + 0.158 0.000 -8.578
ENSG00000138071 E011 945.9677277 0.0001090855 9.836246e-14 1.227348e-11 2 65246524 65246739 216 + 2.867 2.802 -0.218
ENSG00000138071 E012 467.3476009 0.0002415012 3.847306e-04 4.585312e-03 2 65251027 65251099 73 + 2.556 2.526 -0.100
ENSG00000138071 E013 663.7220544 0.0001601304 3.082065e-03 2.391071e-02 2 65253728 65253864 137 + 2.704 2.700 -0.013
ENSG00000138071 E014 607.7424746 0.0001181688 2.454292e-05 4.545954e-04 2 65255545 65255694 150 + 2.641 2.766 0.416
ENSG00000138071 E015 821.9109084 0.0001767006 2.671373e-03 2.140716e-02 2 65261247 65261392 146 + 2.795 2.795 0.001
ENSG00000138071 E016 812.2490716 0.0001281568 2.982288e-04 3.712241e-03 2 65265043 65265175 133 + 2.792 2.783 -0.030
ENSG00000138071 E017 5019.8766298 0.0028513690 3.050245e-05 5.476864e-04 2 65268564 65271253 2690 + 3.558 3.670 0.371