ENSG00000138035

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000415374 ENSG00000138035 HEK293_OSMI2_6hA HEK293_TMG_6hB PNPT1 protein_coding nonsense_mediated_decay 25.83479 6.529334 48.47566 0.8786109 1.259877 2.890342 3.141487 0.8033542 6.904408 0.4436268 0.3699708 3.087650 0.11638333 0.13113333 0.14293333 0.01180000 8.893366e-01 3.019464e-10 TRUE TRUE
ENST00000447944 ENSG00000138035 HEK293_OSMI2_6hA HEK293_TMG_6hB PNPT1 protein_coding protein_coding 25.83479 6.529334 48.47566 0.8786109 1.259877 2.890342 5.651940 2.3475959 6.395333 0.2213601 0.1196705 1.441957 0.30040417 0.36320000 0.13213333 -0.23106667 3.019464e-10 3.019464e-10 FALSE TRUE
ENST00000481066 ENSG00000138035 HEK293_OSMI2_6hA HEK293_TMG_6hB PNPT1 protein_coding retained_intron 25.83479 6.529334 48.47566 0.8786109 1.259877 2.890342 1.162025 0.6462002 2.091712 0.1323861 0.0817274 1.679357 0.07521667 0.10403333 0.04313333 -0.06090000 1.950327e-01 3.019464e-10   FALSE
ENST00000625249 ENSG00000138035 HEK293_OSMI2_6hA HEK293_TMG_6hB PNPT1 protein_coding protein_coding 25.83479 6.529334 48.47566 0.8786109 1.259877 2.890342 1.551019 0.0000000 3.760669 0.0000000 2.0029057 8.558677 0.03472917 0.00000000 0.07573333 0.07573333 5.246988e-01 3.019464e-10   FALSE
MSTRG.18434.4 ENSG00000138035 HEK293_OSMI2_6hA HEK293_TMG_6hB PNPT1 protein_coding   25.83479 6.529334 48.47566 0.8786109 1.259877 2.890342 2.162837 0.2107045 4.951937 0.1336915 0.5115393 4.490716 0.08490417 0.03563333 0.10220000 0.06656667 4.201036e-01 3.019464e-10 FALSE TRUE
MSTRG.18434.5 ENSG00000138035 HEK293_OSMI2_6hA HEK293_TMG_6hB PNPT1 protein_coding   25.83479 6.529334 48.47566 0.8786109 1.259877 2.890342 10.715059 2.2219163 21.565384 0.7248516 1.5153196 3.273031 0.33897917 0.32796667 0.44606667 0.11810000 7.440835e-01 3.019464e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000138035 E001 49.4669286 0.0027364789 4.663628e-11 3.537254e-09 2 55634061 55634568 508 - 1.482 1.875 1.334
ENSG00000138035 E002 50.7182546 0.0013579666 1.528824e-15 2.498840e-13 2 55634569 55634849 281 - 1.478 1.922 1.508
ENSG00000138035 E003 44.0961951 0.0041834769 1.922003e-08 8.460351e-07 2 55634850 55635093 244 - 1.444 1.814 1.258
ENSG00000138035 E004 21.3369680 0.0259157251 1.099068e-01 2.988599e-01 2 55635094 55635141 48 - 1.195 1.382 0.654
ENSG00000138035 E005 28.1504296 0.0013900791 3.211749e-01 5.606909e-01 2 55635142 55635216 75 - 1.329 1.413 0.290
ENSG00000138035 E006 78.3429891 0.0012633050 6.548934e-03 4.245694e-02 2 55635217 55635561 345 - 1.751 1.891 0.471
ENSG00000138035 E007 27.6940172 0.0014571324 1.470017e-01 3.580048e-01 2 55635562 55635578 17 - 1.317 1.437 0.415
ENSG00000138035 E008 197.5004022 0.0003495989 4.315393e-02 1.629467e-01 2 55635579 55636311 733 - 2.158 2.225 0.222
ENSG00000138035 E009 161.9213846 0.0003678159 4.378497e-01 6.571073e-01 2 55636312 55636369 58 - 2.090 2.061 -0.096
ENSG00000138035 E010 135.4047730 0.0003723324 2.974242e-01 5.379415e-01 2 55636370 55636392 23 - 2.015 1.973 -0.142
ENSG00000138035 E011 153.6585789 0.0003488158 5.556708e-01 7.402623e-01 2 55637552 55637599 48 - 2.058 2.080 0.076
ENSG00000138035 E012 178.1198019 0.0002994326 7.757327e-03 4.815993e-02 2 55640627 55640705 79 - 2.109 2.200 0.303
ENSG00000138035 E013 154.0785314 0.0003173035 5.675408e-03 3.811654e-02 2 55643158 55643213 56 - 2.046 2.146 0.337
ENSG00000138035 E014 184.8211821 0.0002976196 7.748744e-01 8.816775e-01 2 55643319 55643412 94 - 2.140 2.151 0.035
ENSG00000138035 E015 102.1356204 0.0045546498 4.899905e-01 6.939921e-01 2 55643413 55643425 13 - 1.893 1.849 -0.147
ENSG00000138035 E016 165.1941581 0.0003706776 7.571368e-01 8.709325e-01 2 55644637 55644720 84 - 2.094 2.106 0.040
ENSG00000138035 E017 18.3204394 0.0900063777 6.936615e-01 8.314489e-01 2 55644721 55645348 628 - 1.171 1.131 -0.144
ENSG00000138035 E018 200.2237097 0.0002518982 2.838573e-01 5.243242e-01 2 55645349 55645432 84 - 2.173 2.209 0.121
ENSG00000138035 E019 184.8212391 0.0002520957 1.743687e-01 3.963514e-01 2 55646259 55646322 64 - 2.136 2.183 0.156
ENSG00000138035 E020 199.0826277 0.0002492490 7.503641e-01 8.669530e-01 2 55646415 55646486 72 - 2.178 2.168 -0.033
ENSG00000138035 E021 182.3158985 0.0003578478 4.089049e-01 6.351678e-01 2 55647347 55647400 54 - 2.144 2.115 -0.095
ENSG00000138035 E022 174.0441922 0.0012751946 7.172681e-01 8.464339e-01 2 55647401 55647453 53 - 2.122 2.108 -0.047
ENSG00000138035 E023 0.1779838 0.0369146421 1.000000e+00   2 55654453 55654500 48 - 0.058 0.001 -6.426
ENSG00000138035 E024 157.4674275 0.0017407316 9.891548e-01 9.963678e-01 2 55654900 55654953 54 - 2.076 2.077 0.001
ENSG00000138035 E025 164.4578744 0.0009484255 5.030343e-01 7.028847e-01 2 55656131 55656220 90 - 2.098 2.072 -0.088
ENSG00000138035 E026 133.7146239 0.0021645574 8.886790e-01 9.459936e-01 2 55656305 55656371 67 - 2.005 2.011 0.020
ENSG00000138035 E027 121.7665619 0.0009881747 8.597077e-01 9.301910e-01 2 55660157 55660193 37 - 1.964 1.972 0.028
ENSG00000138035 E028 126.1344046 0.0025299012 8.829687e-01 9.428143e-01 2 55661956 55662026 71 - 1.981 1.975 -0.021
ENSG00000138035 E029 182.3087839 0.0003715077 1.807346e-02 8.929101e-02 2 55666991 55667093 103 - 2.150 2.064 -0.289
ENSG00000138035 E030 158.6932886 0.0002900757 7.583564e-03 4.741068e-02 2 55667862 55667958 97 - 2.094 1.989 -0.350
ENSG00000138035 E031 134.9435692 0.0003759858 2.674734e-02 1.175243e-01 2 55671319 55671376 58 - 2.020 1.926 -0.315
ENSG00000138035 E032 125.6388323 0.0007099024 9.504347e-02 2.730350e-01 2 55671995 55672046 52 - 1.988 1.915 -0.247
ENSG00000138035 E033 196.7278848 0.0012335111 1.502383e-01 3.626600e-01 2 55672893 55673079 187 - 2.177 2.122 -0.183
ENSG00000138035 E034 168.4173530 0.0004867902 2.629390e-02 1.161873e-01 2 55679682 55679795 114 - 2.115 2.030 -0.285
ENSG00000138035 E035 117.1414759 0.0003371049 6.025659e-02 2.034021e-01 2 55680712 55680759 48 - 1.959 1.875 -0.284
ENSG00000138035 E036 1.2563522 0.0133694893 7.845345e-01   2 55680760 55680854 95 - 0.300 0.238 -0.445
ENSG00000138035 E037 109.4880623 0.0003937603 1.079300e-01 2.956003e-01 2 55680855 55680918 64 - 1.927 1.853 -0.251
ENSG00000138035 E038 99.1321757 0.0003931075 9.171773e-02 2.668777e-01 2 55683785 55683834 50 - 1.888 1.806 -0.276
ENSG00000138035 E039 90.4602131 0.0004713222 2.735874e-02 1.193695e-01 2 55684943 55684993 51 - 1.851 1.736 -0.389
ENSG00000138035 E040 85.1309974 0.0006885019 5.812064e-02 1.985570e-01 2 55684994 55685048 55 - 1.825 1.724 -0.342
ENSG00000138035 E041 89.1691018 0.0030316936 2.867750e-01 5.274886e-01 2 55686370 55686440 71 - 1.838 1.779 -0.200
ENSG00000138035 E042 49.7422382 0.0130569275 4.897468e-01 6.938259e-01 2 55686441 55686444 4 - 1.590 1.535 -0.189
ENSG00000138035 E043 80.0429879 0.0005721737 1.437858e-01 3.530562e-01 2 55687645 55687705 61 - 1.796 1.718 -0.266
ENSG00000138035 E044 122.4124995 0.0040162058 3.525523e-02 1.422565e-01 2 55693663 55693863 201 - 1.983 1.872 -0.373