ENSG00000137812

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000399668 ENSG00000137812 HEK293_OSMI2_6hA HEK293_TMG_6hB KNL1 protein_coding protein_coding 6.53868 2.559544 11.90278 0.3515721 0.3080331 2.212925 4.44559665 0.8780833 8.9195953 0.14209649 0.19072585 3.3298279 0.53677917 0.36473333 0.7507667 0.38603333 0.01521474 0.01521474 FALSE TRUE
ENST00000526913 ENSG00000137812 HEK293_OSMI2_6hA HEK293_TMG_6hB KNL1 protein_coding nonsense_mediated_decay 6.53868 2.559544 11.90278 0.3515721 0.3080331 2.212925 0.65377634 0.7021988 1.7954279 0.23399355 0.36995538 1.3419889 0.12070417 0.25963333 0.1494333 -0.11020000 0.61337152 0.01521474 TRUE TRUE
ENST00000528967 ENSG00000137812 HEK293_OSMI2_6hA HEK293_TMG_6hB KNL1 protein_coding retained_intron 6.53868 2.559544 11.90278 0.3515721 0.3080331 2.212925 0.15578240 0.2433936 0.4762048 0.12820990 0.02474612 0.9401842 0.03911250 0.11293333 0.0401000 -0.07283333 0.84846680 0.01521474   FALSE
ENST00000531626 ENSG00000137812 HEK293_OSMI2_6hA HEK293_TMG_6hB KNL1 protein_coding processed_transcript 6.53868 2.559544 11.90278 0.3515721 0.3080331 2.212925 0.90256618 0.4744658 0.0000000 0.47446580 0.00000000 -5.5983229 0.24665000 0.15616667 0.0000000 -0.15616667 0.74207880 0.01521474   FALSE
ENST00000532347 ENSG00000137812 HEK293_OSMI2_6hA HEK293_TMG_6hB KNL1 protein_coding retained_intron 6.53868 2.559544 11.90278 0.3515721 0.3080331 2.212925 0.08723275 0.1756437 0.0000000 0.08922298 0.00000000 -4.2144647 0.02349167 0.07703333 0.0000000 -0.07703333 0.06497075 0.01521474 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000137812 E001 0.1779838 0.0405016808 1.000000e+00   15 40594020 40594033 14 + 0.055 0.000 -6.921
ENSG00000137812 E002 0.1779838 0.0405016808 1.000000e+00   15 40594034 40594034 1 + 0.055 0.000 -9.086
ENSG00000137812 E003 1.4155550 0.0127417287 6.634347e-01 8.120633e-01 15 40594035 40594248 214 + 0.319 0.247 -0.499
ENSG00000137812 E004 1.2846868 0.0136130748 1.935736e-01   15 40594249 40594264 16 + 0.319 0.000 -12.136
ENSG00000137812 E005 2.4786564 0.1058219773 6.017799e-02 2.032434e-01 15 40594265 40594271 7 + 0.485 0.000 -12.515
ENSG00000137812 E006 21.0496811 0.0204264463 1.357855e-05 2.733463e-04 15 40594272 40594392 121 + 1.262 0.609 -2.496
ENSG00000137812 E007 19.4649920 0.0027327901 1.277602e-03 1.199172e-02 15 40602915 40602966 52 + 1.216 0.854 -1.331
ENSG00000137812 E008 17.0113438 0.0019326846 6.046855e-03 3.995545e-02 15 40605110 40605149 40 + 1.163 0.854 -1.141
ENSG00000137812 E009 18.1821066 0.0037743003 3.100364e-03 2.401976e-02 15 40606393 40606432 40 + 1.190 0.854 -1.239
ENSG00000137812 E010 17.0566226 0.0058318956 7.420727e-03 4.665372e-02 15 40606433 40606452 20 + 1.163 0.853 -1.142
ENSG00000137812 E011 17.6162025 0.0071070558 1.931702e-03 1.661420e-02 15 40608847 40608908 62 + 1.182 0.804 -1.405
ENSG00000137812 E012 15.2032323 0.0069556552 4.960813e-03 3.442125e-02 15 40610245 40610297 53 + 1.112 0.749 -1.371
ENSG00000137812 E013 0.8818932 0.0439084275 3.060502e-02   15 40610799 40610876 78 + 0.104 0.521 3.101
ENSG00000137812 E014 11.4327030 0.0311018030 3.949890e-02 1.534620e-01 15 40611478 40611483 6 + 0.998 0.686 -1.218
ENSG00000137812 E015 12.7715200 0.0332655854 1.985927e-02 9.538865e-02 15 40611484 40611511 28 + 1.044 0.686 -1.386
ENSG00000137812 E016 12.9493316 0.0359225630 3.889524e-04 4.624499e-03 15 40615341 40615378 38 + 1.066 0.404 -2.792
ENSG00000137812 E017 18.3653171 0.0367399903 3.520653e-04 4.259068e-03 15 40618959 40619011 53 + 1.202 0.611 -2.277
ENSG00000137812 E018 308.2066857 0.0265717746 4.749736e-07 1.479755e-05 15 40620640 40623131 2492 + 2.388 2.028 -1.204
ENSG00000137812 E019 381.3765682 0.0030034747 2.756214e-08 1.172223e-06 15 40623132 40625640 2509 + 2.460 2.318 -0.473
ENSG00000137812 E020 2.0158333 0.0118898367 9.231484e-01 9.638600e-01 15 40625641 40626042 402 + 0.396 0.404 0.044
ENSG00000137812 E021 21.6777960 0.0016681541 2.073941e-01 4.386799e-01 15 40628070 40628073 4 + 1.234 1.148 -0.305
ENSG00000137812 E022 49.7609085 0.0068769421 6.578071e-01 8.085267e-01 15 40628074 40628208 135 + 1.563 1.578 0.048
ENSG00000137812 E023 38.3289961 0.0012354942 8.073606e-02 2.463143e-01 15 40628611 40628678 68 + 1.427 1.595 0.576
ENSG00000137812 E024 33.2752733 0.0012506537 8.438654e-02 2.535073e-01 15 40629273 40629323 51 + 1.368 1.541 0.596
ENSG00000137812 E025 36.3257525 0.0013856620 5.229607e-01 7.170311e-01 15 40629324 40629371 48 + 1.418 1.512 0.320
ENSG00000137812 E026 50.8668881 0.0221471000 6.271028e-01 7.886057e-01 15 40640912 40641027 116 + 1.574 1.577 0.008
ENSG00000137812 E027 43.6622293 0.0068272312 1.040782e-01 2.889326e-01 15 40644997 40645087 91 + 1.523 1.433 -0.308
ENSG00000137812 E028 50.5197155 0.0096574758 6.800559e-01 8.227363e-01 15 40645656 40645772 117 + 1.569 1.579 0.034
ENSG00000137812 E029 0.6600180 0.0193874923 5.750653e-01   15 40646886 40646986 101 + 0.188 0.000 -11.175
ENSG00000137812 E030 61.5294053 0.0180410630 5.664545e-01 7.476368e-01 15 40646987 40647074 88 + 1.640 1.728 0.301
ENSG00000137812 E031 0.8315845 0.3059906579 6.960647e-02   15 40650127 40650300 174 + 0.106 0.518 3.054
ENSG00000137812 E032 49.7610909 0.0142160329 1.426673e-02 7.540440e-02 15 40650301 40650378 78 + 1.519 1.760 0.821
ENSG00000137812 E033 40.8762300 0.0066794663 4.930467e-02 1.779421e-01 15 40650544 40650583 40 + 1.447 1.643 0.667
ENSG00000137812 E034 58.8135863 0.0022312490 2.205373e-01 4.541489e-01 15 40651471 40651572 102 + 1.617 1.738 0.410
ENSG00000137812 E035 60.0101497 0.0007757577 1.672641e-01 3.869266e-01 15 40652005 40652105 101 + 1.625 1.750 0.424
ENSG00000137812 E036 46.9232634 0.0008505018 6.665091e-03 4.302276e-02 15 40654909 40654977 69 + 1.507 1.720 0.724
ENSG00000137812 E037 49.3253154 0.0007980760 5.392541e-02 1.889214e-01 15 40657042 40657151 110 + 1.536 1.700 0.560
ENSG00000137812 E038 41.3395448 0.0010102304 2.974603e-03 2.324266e-02 15 40657355 40657473 119 + 1.448 1.687 0.813
ENSG00000137812 E039 41.7399355 0.0715454569 3.850970e-02 1.508978e-01 15 40659339 40659461 123 + 1.435 1.731 1.013
ENSG00000137812 E040 93.3566198 1.3937452543 4.610857e-01 6.738904e-01 15 40662074 40662572 499 + 1.787 2.031 0.821
ENSG00000137812 E041 28.0065143 0.7450872134 2.341750e-01 4.699322e-01 15 40662573 40662678 106 + 1.241 1.626 1.329
ENSG00000137812 E042 14.3158572 0.5047214456 1.245530e-01 3.230246e-01 15 40662679 40662683 5 + 0.947 1.399 1.614
ENSG00000137812 E043 66.7456745 0.9578240008 1.757234e-01 3.980722e-01 15 40662684 40663334 651 + 1.573 2.055 1.629
ENSG00000137812 E044 12.8520342 0.4329162403 1.143603e-01 3.064069e-01 15 40663335 40663341 7 + 0.917 1.333 1.499
ENSG00000137812 E045 101.3729273 1.3834093070 2.835528e-01 5.239974e-01 15 40663342 40664342 1001 + 1.760 2.217 1.534