ENSG00000137414

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000259963 ENSG00000137414 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM8A1 protein_coding protein_coding 19.777 4.443431 37.9234 0.7724072 0.688473 3.09048 9.487324 2.688718 11.00971 0.2917127 0.5849802 2.029740 0.4609792 0.6264333 0.2906 -0.3358333 0.008335444 0.008335444 FALSE TRUE
ENST00000690940 ENSG00000137414 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM8A1 protein_coding protein_coding 19.777 4.443431 37.9234 0.7724072 0.688473 3.09048 9.622133 1.717689 25.44732 0.5399127 0.9429724 3.881165 0.5047042 0.3640333 0.6709 0.3068667 0.041067243 0.008335444 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000137414 E001 6.2034732 0.0065039314 1.299162e-01 3.317009e-01 6 17600302 17600362 61 + 0.701 0.902 0.794
ENSG00000137414 E002 25.5305469 0.0052215874 1.953504e-04 2.624188e-03 6 17600363 17600405 43 + 1.235 1.525 1.004
ENSG00000137414 E003 26.7444652 0.0022031426 2.589852e-04 3.313368e-03 6 17600406 17600409 4 + 1.259 1.526 0.924
ENSG00000137414 E004 45.6992694 0.0007840983 4.183933e-04 4.910094e-03 6 17600410 17600541 132 + 1.494 1.691 0.672
ENSG00000137414 E005 24.7722950 0.0019227230 4.716478e-04 5.418159e-03 6 17600542 17600581 40 + 1.219 1.484 0.922
ENSG00000137414 E006 48.5524262 0.0020515320 2.189914e-06 5.650223e-05 6 17600582 17600882 301 + 1.498 1.766 0.913
ENSG00000137414 E007 40.9914044 0.0011893446 1.024545e-04 1.526374e-03 6 17600883 17601004 122 + 1.438 1.670 0.792
ENSG00000137414 E008 61.4659210 0.0007870745 8.346824e-01 9.163850e-01 6 17601005 17601121 117 + 1.661 1.616 -0.151
ENSG00000137414 E009 0.1779838 0.0343432521 9.115307e-01   6 17601161 17601485 325 + 0.055 0.000 -6.993
ENSG00000137414 E010 101.8203206 0.0022995551 8.449907e-02 2.537341e-01 6 17602590 17602710 121 + 1.886 1.755 -0.443
ENSG00000137414 E011 0.7485225 0.2495567852 6.978058e-01   6 17602711 17602881 171 + 0.220 0.001 -8.645
ENSG00000137414 E012 124.2732946 0.0003820595 4.459511e-02 1.665921e-01 6 17604906 17605029 124 + 1.971 1.845 -0.422
ENSG00000137414 E013 142.2828094 0.0019239495 9.682679e-01 9.859793e-01 6 17605874 17606013 140 + 2.020 1.989 -0.103
ENSG00000137414 E014 2.8372670 0.0079778513 7.325639e-01 8.557371e-01 6 17606014 17606169 156 + 0.498 0.407 -0.468
ENSG00000137414 E015 1817.7097804 0.0021859402 3.691083e-04 4.428335e-03 6 17608195 17611719 3525 + 3.124 3.055 -0.231