ENSG00000136628

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366923 ENSG00000136628 HEK293_OSMI2_6hA HEK293_TMG_6hB EPRS1 protein_coding protein_coding 84.26709 26.76592 143.6726 7.283683 3.336448 2.423877 75.917643 25.582416 128.729848 7.0496990 5.5930636 2.3306704 0.88673750 0.95356667 0.89533333 -0.05823333 6.573406e-01 2.386286e-13 FALSE TRUE
ENST00000464052 ENSG00000136628 HEK293_OSMI2_6hA HEK293_TMG_6hB EPRS1 protein_coding retained_intron 84.26709 26.76592 143.6726 7.283683 3.336448 2.423877 5.258551 0.000000 12.228854 0.0000000 2.2530911 10.2572527 0.03654167 0.00000000 0.08556667 0.08556667 2.386286e-13 2.386286e-13 FALSE FALSE
ENST00000468487 ENSG00000136628 HEK293_OSMI2_6hA HEK293_TMG_6hB EPRS1 protein_coding processed_transcript 84.26709 26.76592 143.6726 7.283683 3.336448 2.423877 2.748543 1.146677 1.675152 0.8270804 0.3150298 0.5428934 0.07327500 0.04536667 0.01163333 -0.03373333 9.097425e-01 2.386286e-13   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000136628 E001 1.6906351 0.0121989077 1.778641e-03 1.555753e-02 1 219968600 219968602 3 - 0.207 0.760 2.959
ENSG00000136628 E002 145.5532246 0.0011968714 2.033343e-11 1.662534e-09 1 219968603 219968688 86 - 1.991 2.269 0.931
ENSG00000136628 E003 644.9666027 0.0046722758 4.434273e-12 4.081526e-10 1 219968689 219968956 268 - 2.631 2.922 0.970
ENSG00000136628 E004 551.7474779 0.0022811379 1.840917e-14 2.605251e-12 1 219969058 219969122 65 - 2.575 2.830 0.848
ENSG00000136628 E005 7.0747204 0.0089338416 9.012217e-01 9.524792e-01 1 219969123 219969247 125 - 0.803 0.810 0.027
ENSG00000136628 E006 587.7616147 0.0035214811 6.509780e-11 4.787832e-09 1 219972069 219972147 79 - 2.602 2.855 0.843
ENSG00000136628 E007 875.6475870 0.0025668314 8.557547e-12 7.452658e-10 1 219973238 219973398 161 - 2.783 3.009 0.754
ENSG00000136628 E008 920.9586694 0.0027028265 7.160752e-07 2.123381e-05 1 219978546 219978719 174 - 2.817 2.998 0.600
ENSG00000136628 E009 910.3771370 0.0001063715 2.021883e-18 4.908401e-16 1 219979418 219979615 198 - 2.819 2.978 0.528
ENSG00000136628 E010 659.8940530 0.0010908793 2.013639e-06 5.253683e-05 1 219980085 219980240 156 - 2.684 2.825 0.470
ENSG00000136628 E011 508.8263762 0.0001520273 2.408132e-07 8.120654e-06 1 219980756 219980857 102 - 2.574 2.706 0.441
ENSG00000136628 E012 436.2403200 0.0001574520 2.852267e-03 2.251258e-02 1 219981378 219981457 80 - 2.516 2.612 0.320
ENSG00000136628 E013 0.6765862 0.2360031666 5.471044e-01   1 219982510 219982771 262 - 0.210 0.000 -10.973
ENSG00000136628 E014 455.5965201 0.0001469061 3.571662e-02 1.434773e-01 1 219982772 219982844 73 - 2.538 2.615 0.255
ENSG00000136628 E015 722.9018987 0.0007365626 5.396205e-03 3.667784e-02 1 219983189 219983398 210 - 2.736 2.823 0.289
ENSG00000136628 E016 340.8175983 0.0002120325 1.863594e-03 1.614782e-02 1 219984206 219984257 52 - 2.405 2.511 0.355
ENSG00000136628 E017 0.9475002 0.0153787590 2.431948e-01   1 219984258 219984258 1 - 0.283 0.000 -11.537
ENSG00000136628 E018 525.7969436 0.0001436166 1.054272e-04 1.564075e-03 1 219987142 219987295 154 - 2.594 2.701 0.356
ENSG00000136628 E019 479.0359050 0.0013712143 1.355656e-01 3.406854e-01 1 219987296 219987404 109 - 2.562 2.633 0.236
ENSG00000136628 E020 666.6976275 0.0005733600 7.543899e-02 2.357333e-01 1 219988590 219988823 234 - 2.706 2.771 0.219
ENSG00000136628 E021 556.7981521 0.0001349743 4.586215e-01 6.722467e-01 1 219996983 219997175 193 - 2.630 2.677 0.157
ENSG00000136628 E022 616.1671825 0.0001015729 5.640918e-04 6.256802e-03 1 219997176 219997342 167 - 2.690 2.659 -0.102
ENSG00000136628 E023 453.4657882 0.0004400445 7.205437e-03 4.560938e-02 1 220001138 220001200 63 - 2.555 2.525 -0.102
ENSG00000136628 E024 430.3746631 0.0002091616 6.541087e-04 7.043479e-03 1 220001201 220001255 55 - 2.535 2.490 -0.150
ENSG00000136628 E025 588.8541938 0.0008370369 7.841978e-04 8.146875e-03 1 220005248 220005360 113 - 2.672 2.624 -0.158
ENSG00000136628 E026 809.1859257 0.0001218967 3.548662e-07 1.143972e-05 1 220006106 220006313 208 - 2.812 2.761 -0.171
ENSG00000136628 E027 646.2685694 0.0003264923 5.630935e-09 2.792822e-07 1 220007202 220007338 137 - 2.719 2.636 -0.278
ENSG00000136628 E028 1.7680086 0.0338581582 6.757401e-01 8.200094e-01 1 220007339 220007365 27 - 0.375 0.481 0.563
ENSG00000136628 E029 489.7756189 0.0005197546 4.298872e-07 1.355997e-05 1 220010946 220011056 111 - 2.600 2.513 -0.290
ENSG00000136628 E030 0.1426347 0.0335025250 1.000000e+00   1 220018126 220018146 21 - 0.062 0.000 -8.951
ENSG00000136628 E031 310.1716078 0.0001644059 8.110694e-06 1.749279e-04 1 220018449 220018508 60 - 2.402 2.316 -0.288
ENSG00000136628 E032 398.3185496 0.0035438559 2.136977e-02 1.005587e-01 1 220018995 220019079 85 - 2.505 2.453 -0.174
ENSG00000136628 E033 697.9406443 0.0008042047 8.080230e-09 3.877392e-07 1 220019988 220020221 234 - 2.756 2.658 -0.327
ENSG00000136628 E034 567.0415204 0.0001837210 5.639316e-13 6.054021e-11 1 220022347 220022518 172 - 2.668 2.552 -0.387
ENSG00000136628 E035 569.7876351 0.0002544021 1.641824e-21 5.748855e-19 1 220024264 220024456 193 - 2.679 2.505 -0.577
ENSG00000136628 E036 519.6061034 0.0003076293 9.650452e-25 4.792014e-22 1 220025132 220025258 127 - 2.643 2.437 -0.687
ENSG00000136628 E037 538.1402622 0.0002028955 2.095781e-30 1.719380e-27 1 220030386 220030480 95 - 2.659 2.435 -0.748
ENSG00000136628 E038 590.2125372 0.0001069793 5.809665e-34 6.490886e-31 1 220032387 220032526 140 - 2.698 2.477 -0.736
ENSG00000136628 E039 577.3908471 0.0001459946 2.285701e-24 1.093421e-21 1 220033502 220033658 157 - 2.685 2.505 -0.599
ENSG00000136628 E040 412.4195073 0.0001849536 3.319669e-15 5.195909e-13 1 220034914 220035013 100 - 2.537 2.378 -0.531
ENSG00000136628 E041 414.6944854 0.0002702006 8.822862e-25 4.419838e-22 1 220040185 220040269 85 - 2.549 2.317 -0.774
ENSG00000136628 E042 363.9799070 0.0001662919 1.855673e-19 5.010111e-17 1 220046343 220046615 273 - 2.488 2.282 -0.687