ENSG00000136448

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258960 ENSG00000136448 HEK293_OSMI2_6hA HEK293_TMG_6hB NMT1 protein_coding protein_coding 65.6524 67.05068 68.32124 7.495956 3.631167 0.02707824 29.184808 36.102128 20.78062 6.288880 1.0744743 -0.7965510 0.4408833 0.5330000 0.3054667 -0.22753333 0.01217206 0.01217206 FALSE TRUE
ENST00000590114 ENSG00000136448 HEK293_OSMI2_6hA HEK293_TMG_6hB NMT1 protein_coding nonsense_mediated_decay 65.6524 67.05068 68.32124 7.495956 3.631167 0.02707824 9.639928 4.612517 17.13504 2.314405 0.4212679 1.8910407 0.1496042 0.0757000 0.2518333 0.17613333 0.64718022 0.01217206 FALSE TRUE
ENST00000676828 ENSG00000136448 HEK293_OSMI2_6hA HEK293_TMG_6hB NMT1 protein_coding nonsense_mediated_decay 65.6524 67.05068 68.32124 7.495956 3.631167 0.02707824 16.444384 17.096006 13.82846 3.665397 1.4456474 -0.3058193 0.2471417 0.2484333 0.2018000 -0.04663333 0.76766192 0.01217206 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000136448 E001 0.0000000       17 44957992 44958062 71 +      
ENSG00000136448 E002 0.0000000       17 45051610 45051693 84 +      
ENSG00000136448 E003 0.1426347 0.0317361817 3.459473e-01   17 45061271 45061282 12 + 0.143 0.000 -9.749
ENSG00000136448 E004 1.4644356 0.0123162737 3.887316e-01 6.194442e-01 17 45061283 45061300 18 + 0.471 0.315 -0.881
ENSG00000136448 E005 1.4644356 0.0123162737 3.887316e-01 6.194442e-01 17 45061301 45061303 3 + 0.471 0.315 -0.881
ENSG00000136448 E006 1.9456619 0.0105725904 5.369792e-01 7.270014e-01 17 45061304 45061305 2 + 0.525 0.414 -0.561
ENSG00000136448 E007 20.5909112 0.0018537964 4.458820e-01 6.626855e-01 17 45061306 45061316 11 + 1.361 1.292 -0.238
ENSG00000136448 E008 49.1915031 0.0286809246 2.998280e-01 5.402579e-01 17 45061317 45061319 3 + 1.755 1.643 -0.380
ENSG00000136448 E009 66.1074984 0.0185670399 1.304699e-01 3.325596e-01 17 45061320 45061329 10 + 1.892 1.761 -0.443
ENSG00000136448 E010 76.8260368 0.0194539948 1.233605e-01 3.210841e-01 17 45061330 45061333 4 + 1.958 1.825 -0.447
ENSG00000136448 E011 286.9907615 0.0091970245 1.996042e-01 4.291674e-01 17 45061334 45061460 127 + 2.494 2.420 -0.248
ENSG00000136448 E012 1.0398556 1.0078240541 7.725342e-01   17 45061679 45061910 232 + 0.147 0.377 1.780
ENSG00000136448 E013 0.0000000       17 45078083 45078149 67 +      
ENSG00000136448 E014 326.9374580 0.0137541471 6.283343e-01 7.894880e-01 17 45081644 45081752 109 + 2.495 2.515 0.066
ENSG00000136448 E015 2.0833073 0.0101342160 3.624982e-01 5.977674e-01 17 45081753 45081995 243 + 0.573 0.414 -0.781
ENSG00000136448 E016 1.0107361 0.0459902626 6.818280e-01   17 45083658 45083755 98 + 0.337 0.256 -0.550
ENSG00000136448 E017 475.6182409 0.0040963189 5.209480e-01 7.155279e-01 17 45086508 45086652 145 + 2.688 2.656 -0.107
ENSG00000136448 E018 0.7558267 0.0506368016 5.163774e-01   17 45093631 45093684 54 + 0.144 0.256 1.034
ENSG00000136448 E019 497.3581468 0.0005771603 9.502541e-04 9.505508e-03 17 45093685 45093803 119 + 2.730 2.656 -0.244
ENSG00000136448 E020 409.5041914 0.0016465902 1.678495e-01 3.877294e-01 17 45096194 45096285 92 + 2.628 2.586 -0.140
ENSG00000136448 E021 462.9812754 0.0045072659 3.493569e-02 1.414106e-01 17 45097128 45097244 117 + 2.701 2.624 -0.256
ENSG00000136448 E022 654.4087515 0.0014242056 1.782541e-04 2.428142e-03 17 45098382 45098552 171 + 2.857 2.768 -0.295
ENSG00000136448 E023 2.3777474 0.1306668957 6.982029e-01 8.344071e-01 17 45098553 45098843 291 + 0.574 0.493 -0.381
ENSG00000136448 E024 589.5483784 0.0003254740 4.745176e-08 1.903260e-06 17 45099405 45099513 109 + 2.816 2.717 -0.328
ENSG00000136448 E025 17.1058542 0.0023363429 6.975331e-01 8.339433e-01 17 45099514 45099819 306 + 1.232 1.255 0.082
ENSG00000136448 E026 761.1765133 0.0003121951 8.602011e-06 1.841214e-04 17 45102951 45103121 171 + 2.915 2.841 -0.247
ENSG00000136448 E027 2.3700991 0.0643813727 5.658167e-01 7.472422e-01 17 45103122 45103708 587 + 0.574 0.459 -0.551
ENSG00000136448 E028 792.4878562 0.0011163435 2.661260e-03 2.134526e-02 17 45103709 45103876 168 + 2.928 2.863 -0.217
ENSG00000136448 E029 38.7302637 0.0659853102 3.332535e-01 5.718674e-01 17 45103877 45104120 244 + 1.664 1.534 -0.444
ENSG00000136448 E030 22.1903552 0.0325335710 4.724220e-01 6.818464e-01 17 45104121 45104224 104 + 1.406 1.316 -0.313
ENSG00000136448 E031 86.8495348 0.0815271859 2.489889e-01 4.867675e-01 17 45104225 45104858 634 + 2.019 1.869 -0.503
ENSG00000136448 E032 676.9498847 0.0023029423 1.997947e-01 4.294161e-01 17 45104859 45104996 138 + 2.843 2.806 -0.124
ENSG00000136448 E033 54.4421126 0.0748233655 8.334976e-01 9.157027e-01 17 45104997 45105618 622 + 1.703 1.754 0.175
ENSG00000136448 E034 309.4750556 0.0023749890 4.042816e-01 6.317635e-01 17 45105619 45105643 25 + 2.501 2.469 -0.106
ENSG00000136448 E035 728.4337773 0.0019799697 6.930227e-01 8.310767e-01 17 45105644 45105877 234 + 2.862 2.848 -0.046
ENSG00000136448 E036 283.1752583 0.0001750853 7.198763e-01 8.481210e-01 17 45105878 45105945 68 + 2.452 2.435 -0.058
ENSG00000136448 E037 1739.5149813 0.0012465130 2.172006e-05 4.098295e-04 17 45105946 45107778 1833 + 3.189 3.255 0.218
ENSG00000136448 E038 1218.6977558 0.0001215195 2.587046e-55 1.098350e-51 17 45107779 45108677 899 + 2.966 3.133 0.553
ENSG00000136448 E039 335.0842337 0.0001618843 1.388752e-19 3.847341e-17 17 45108678 45109103 426 + 2.398 2.577 0.598