ENSG00000136156

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000607866 ENSG00000136156 HEK293_OSMI2_6hA HEK293_TMG_6hB ITM2B protein_coding nonsense_mediated_decay 103.7924 74.46627 127.2775 11.35989 3.454489 0.7732376 36.34573 23.180471 59.911271 3.161647 3.5503025 1.3695361 0.34820000 0.3148333 0.47016667 0.15533333 3.741654e-02 8.626799e-12 FALSE FALSE
ENST00000648898 ENSG00000136156 HEK293_OSMI2_6hA HEK293_TMG_6hB ITM2B protein_coding processed_transcript 103.7924 74.46627 127.2775 11.35989 3.454489 0.7732376 19.74695 10.441666 30.510500 2.550479 1.5614303 1.5460457 0.19352083 0.1367667 0.24000000 0.10323333 1.209997e-01 8.626799e-12 FALSE TRUE
ENST00000649452 ENSG00000136156 HEK293_OSMI2_6hA HEK293_TMG_6hB ITM2B protein_coding nonsense_mediated_decay 103.7924 74.46627 127.2775 11.35989 3.454489 0.7732376 10.27905 10.803912 4.696285 1.760279 0.4654448 -1.2002280 0.09995417 0.1458333 0.03676667 -0.10906667 8.626799e-12 8.626799e-12 TRUE TRUE
ENST00000650479 ENSG00000136156 HEK293_OSMI2_6hA HEK293_TMG_6hB ITM2B protein_coding nonsense_mediated_decay 103.7924 74.46627 127.2775 11.35989 3.454489 0.7732376 25.44377 22.048955 15.734032 5.166461 0.4192995 -0.4865594 0.24615833 0.2951000 0.12383333 -0.17126667 5.561688e-03 8.626799e-12 FALSE FALSE
MSTRG.8738.3 ENSG00000136156 HEK293_OSMI2_6hA HEK293_TMG_6hB ITM2B protein_coding   103.7924 74.46627 127.2775 11.35989 3.454489 0.7732376 10.91937 7.522786 14.912979 1.400533 0.1772813 0.9862802 0.10264167 0.1004333 0.11726667 0.01683333 7.469323e-01 8.626799e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000136156 E001 0.0000000       13 48232612 48232698 87 +      
ENSG00000136156 E002 23.5133250 0.0235173459 1.133880e-05 2.336029e-04 13 48233188 48233196 9 + 1.128 1.578 1.568
ENSG00000136156 E003 29.8796715 0.0138829850 5.847610e-07 1.780632e-05 13 48233197 48233202 6 + 1.244 1.673 1.479
ENSG00000136156 E004 31.6823235 0.0134173337 2.581601e-07 8.642128e-06 13 48233203 48233205 3 + 1.265 1.698 1.489
ENSG00000136156 E005 51.3328404 0.0008422202 2.254585e-18 5.430929e-16 13 48233206 48233218 13 + 1.465 1.909 1.506
ENSG00000136156 E006 507.1238224 0.0001603718 2.320935e-25 1.220273e-22 13 48233219 48233477 259 + 2.627 2.789 0.536
ENSG00000136156 E007 0.8923043 0.2113568493 5.475752e-01   13 48233599 48233600 2 + 0.203 0.318 0.860
ENSG00000136156 E008 1.2564542 0.1912112051 6.037257e-01   13 48233601 48233645 45 + 0.277 0.390 0.706
ENSG00000136156 E009 0.2027342 0.0392305981 3.979705e-01   13 48233646 48233653 8 + 0.000 0.132 10.567
ENSG00000136156 E010 0.5067846 0.0213250752 6.978946e-01   13 48233930 48234013 84 + 0.200 0.132 -0.723
ENSG00000136156 E011 0.6907378 0.5603602071 8.384892e-01   13 48234014 48234071 58 + 0.196 0.245 0.408
ENSG00000136156 E012 0.7132229 0.0776805146 5.658025e-01   13 48239226 48239332 107 + 0.201 0.311 0.837
ENSG00000136156 E013 472.3455498 0.0013267345 1.463151e-01 3.568530e-01 13 48253808 48253874 67 + 2.664 2.690 0.084
ENSG00000136156 E014 463.2347350 0.0001761357 2.507752e-01 4.888023e-01 13 48253875 48253936 62 + 2.680 2.655 -0.081
ENSG00000136156 E015 0.4764247 0.0215771505 6.983039e-01   13 48254900 48254970 71 + 0.200 0.132 -0.724
ENSG00000136156 E016 0.0000000       13 48254971 48254976 6 +      
ENSG00000136156 E017 0.1308682 0.0326491905 4.007051e-01   13 48255337 48255437 101 + 0.000 0.132 10.560
ENSG00000136156 E018 580.7824092 0.0001131024 5.387979e-02 1.888083e-01 13 48256177 48256268 92 + 2.781 2.748 -0.111
ENSG00000136156 E019 808.5303093 0.0001037673 1.218080e-05 2.487470e-04 13 48256269 48256383 115 + 2.935 2.876 -0.198
ENSG00000136156 E020 912.6962726 0.0001262709 1.228571e-01 3.202499e-01 13 48258126 48258236 111 + 2.972 2.949 -0.080
ENSG00000136156 E021 994.5018529 0.0001354896 5.623329e-01 7.449417e-01 13 48258797 48258935 139 + 3.004 2.992 -0.042
ENSG00000136156 E022 404.8750898 0.0001705928 3.820694e-02 1.501356e-01 13 48258936 48258947 12 + 2.593 2.625 0.105
ENSG00000136156 E023 765.4548682 0.0009451818 5.853187e-01 7.605014e-01 13 48261139 48261321 183 + 2.892 2.874 -0.061
ENSG00000136156 E024 618.7150963 0.0028665276 3.814414e-06 9.114608e-05 13 48261322 48262570 1249 + 2.849 2.710 -0.461
ENSG00000136156 E025 11.8364147 0.0901096840 5.487668e-01 7.354844e-01 13 48262571 48270357 7787 + 1.057 1.148 0.326