ENSG00000135945

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258428 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding protein_coding 8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 1.3041248 1.11788494 0.0000000 0.28007041 0.0000000 -6.8174761 0.20515000 0.30933333 0.00000000 -0.30933333 7.859103e-11 7.859103e-11 FALSE TRUE
ENST00000393445 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding protein_coding 8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 1.4262372 0.36770503 2.7876849 0.36770503 1.3938589 2.8889016 0.16525833 0.09980000 0.20146667 0.10166667 7.308183e-01 7.859103e-11 FALSE TRUE
ENST00000413697 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding nonsense_mediated_decay 8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 0.6530328 0.33695864 0.9916575 0.02424844 0.0997568 1.5295537 0.07383750 0.09153333 0.06666667 -0.02486667 7.359733e-01 7.859103e-11 TRUE TRUE
ENST00000465086 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding retained_intron 8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 0.5381273 0.22342260 1.0254174 0.15657872 0.2137024 2.1491964 0.05730000 0.05810000 0.06720000 0.00910000 8.842212e-01 7.859103e-11   FALSE
ENST00000465835 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding processed_transcript 8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 1.2683967 0.09486917 2.8888172 0.09486917 0.3914428 4.7888018 0.09545000 0.02783333 0.19346667 0.16563333 1.445669e-01 7.859103e-11 FALSE TRUE
ENST00000473819 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding retained_intron 8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 0.5450340 0.19223690 1.0494204 0.09787776 0.3676931 2.3891571 0.05613333 0.05403333 0.06776667 0.01373333 9.023363e-01 7.859103e-11 FALSE TRUE
ENST00000477121 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding retained_intron 8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 0.4003263 0.42306126 0.6313972 0.22502250 0.2271426 0.5666469 0.05014583 0.10963333 0.04073333 -0.06890000 6.492045e-01 7.859103e-11 FALSE FALSE
MSTRG.18938.4 ENSG00000135945 HEK293_OSMI2_6hA HEK293_TMG_6hB REV1 protein_coding   8.960835 3.693604 14.94225 0.1678975 1.184973 2.013361 0.7816736 0.26830366 1.2215234 0.10670108 0.4159684 2.1457122 0.08187917 0.07160000 0.08193333 0.01033333 9.852190e-01 7.859103e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135945 E001 0.2027342 0.0356319701 0.1558436394   2 99400475 99400476 2 - 0.000 0.197 11.100
ENSG00000135945 E002 12.6226110 0.4861535866 0.2566546533 0.495424152 2 99400477 99400775 299 - 0.984 1.276 1.050
ENSG00000135945 E003 12.5842230 0.4549347145 0.1233059857 0.320999230 2 99400776 99400887 112 - 0.916 1.365 1.611
ENSG00000135945 E004 7.0737928 0.1788856459 0.0063887696 0.041631558 2 99400888 99400937 50 - 0.620 1.220 2.300
ENSG00000135945 E005 3.2375380 0.2025966753 0.0230271535 0.106039923 2 99400938 99400949 12 - 0.383 0.936 2.433
ENSG00000135945 E006 33.8379187 0.7251634972 0.1634594689 0.381888073 2 99400950 99401285 336 - 1.322 1.766 1.523
ENSG00000135945 E007 32.6990798 0.0665587222 0.0915624898 0.266581233 2 99401286 99401352 67 - 1.396 1.635 0.819
ENSG00000135945 E008 9.5395730 0.3094522658 0.6557647355 0.807178801 2 99401470 99402243 774 - 0.991 0.858 -0.503
ENSG00000135945 E009 24.7980382 0.0137154569 0.1488782526 0.360700652 2 99402244 99402258 15 - 1.308 1.465 0.545
ENSG00000135945 E010 36.4417833 0.0158234767 0.3324256676 0.571195380 2 99402259 99402346 88 - 1.481 1.591 0.378
ENSG00000135945 E011 12.9698069 0.1736502564 0.9540545261 0.978957492 2 99402347 99402529 183 - 1.076 1.100 0.087
ENSG00000135945 E012 8.4909310 0.2265529540 0.9487437240 0.976606005 2 99402530 99402643 114 - 0.890 0.992 0.381
ENSG00000135945 E013 51.1997164 0.0175738666 0.2501670446 0.488143648 2 99402644 99402800 157 - 1.620 1.740 0.406
ENSG00000135945 E014 44.9014959 0.0010973197 0.0148275102 0.077414787 2 99402889 99403039 151 - 1.548 1.717 0.575
ENSG00000135945 E015 18.7933979 0.0021875464 0.0100456923 0.058348491 2 99403040 99403055 16 - 1.154 1.400 0.863
ENSG00000135945 E016 26.2973365 0.0014355316 0.0007654071 0.007989165 2 99403056 99403106 51 - 1.286 1.556 0.932
ENSG00000135945 E017 7.4704714 0.2117336353 0.4442143540 0.661543713 2 99403107 99403430 324 - 0.900 0.748 -0.596
ENSG00000135945 E018 41.8927506 0.0157692860 0.0150139128 0.078135196 2 99403695 99403815 121 - 1.496 1.721 0.765
ENSG00000135945 E019 75.4261828 0.0039464752 0.9422805019 0.973406035 2 99404444 99404677 234 - 1.809 1.834 0.085
ENSG00000135945 E020 33.3212964 0.0071292386 0.1454313304 0.355555337 2 99405910 99405927 18 - 1.490 1.399 -0.310
ENSG00000135945 E021 29.0620438 0.0144118574 0.1666007924 0.386005326 2 99405928 99405932 5 - 1.437 1.333 -0.358
ENSG00000135945 E022 57.8623178 0.0015550959 0.0134974140 0.072372169 2 99405933 99406083 151 - 1.730 1.620 -0.373
ENSG00000135945 E023 27.3089609 0.0015402116 0.1362414336 0.341731467 2 99406084 99406106 23 - 1.410 1.323 -0.300
ENSG00000135945 E024 3.3053013 0.2338171466 0.6915782249 0.830116938 2 99406107 99406324 218 - 0.562 0.638 0.339
ENSG00000135945 E025 43.9766453 0.0007955501 0.0980214595 0.278301038 2 99406325 99406431 107 - 1.606 1.535 -0.244
ENSG00000135945 E026 28.2862538 0.0013189301 0.3249399202 0.564143617 2 99406432 99406490 59 - 1.413 1.368 -0.154
ENSG00000135945 E027 6.4113167 0.1336598129 0.5921518376 0.765088550 2 99406491 99406928 438 - 0.834 0.704 -0.521
ENSG00000135945 E028 41.5728215 0.0008795916 0.1218087946 0.318735515 2 99408029 99408131 103 - 1.580 1.512 -0.232
ENSG00000135945 E029 5.4544124 0.3184613411 0.3377698183 0.576102882 2 99408132 99408403 272 - 0.614 1.009 1.565
ENSG00000135945 E030 50.0850474 0.0007842235 0.0209991142 0.099343471 2 99410695 99410867 173 - 1.668 1.563 -0.359
ENSG00000135945 E031 46.9054202 0.0010970893 0.0782266980 0.241245983 2 99412731 99412840 110 - 1.633 1.556 -0.261
ENSG00000135945 E032 48.8952554 0.0010433212 0.0369784879 0.146851197 2 99412841 99412951 111 - 1.657 1.563 -0.322
ENSG00000135945 E033 0.6600180 0.0193874923 0.2744079603   2 99412952 99412953 2 - 0.237 0.000 -14.033
ENSG00000135945 E034 29.5192805 0.0012582139 0.0404633062 0.155932357 2 99418828 99418831 4 - 1.451 1.324 -0.440
ENSG00000135945 E035 55.0305803 0.0007238881 0.0095283690 0.056094649 2 99418832 99418947 116 - 1.711 1.596 -0.393
ENSG00000135945 E036 58.8159453 0.0008997867 0.0053278525 0.036331187 2 99421499 99421653 155 - 1.738 1.614 -0.421
ENSG00000135945 E037 43.2887430 0.0010500401 0.0276619876 0.120285802 2 99424152 99424280 129 - 1.606 1.497 -0.375
ENSG00000135945 E038 2.4612804 0.0438514750 0.2796442908 0.519743821 2 99424281 99424634 354 - 0.528 0.331 -1.053
ENSG00000135945 E039 0.8018448 0.0178328900 0.4820629040   2 99424781 99424791 11 - 0.189 0.332 1.073
ENSG00000135945 E040 1.9257132 0.0918494526 0.1241419771 0.322288241 2 99424792 99424899 108 - 0.322 0.644 1.632
ENSG00000135945 E041 30.2590442 0.0012751385 0.0426987358 0.161768765 2 99429840 99429945 106 - 1.459 1.335 -0.428
ENSG00000135945 E042 5.8040804 0.0052596926 0.8333293514 0.915629982 2 99429946 99429948 3 - 0.771 0.828 0.223
ENSG00000135945 E043 2.2295262 0.0404516869 0.7827637126 0.886267631 2 99431741 99431800 60 - 0.448 0.518 0.346
ENSG00000135945 E044 3.9177762 0.0391486075 0.5085298011 0.706851482 2 99431801 99431940 140 - 0.610 0.746 0.570
ENSG00000135945 E045 30.4418615 0.0012487132 0.6812172137 0.823419701 2 99434332 99434448 117 - 1.436 1.437 0.003
ENSG00000135945 E046 0.6072928 0.0195629451 0.2969878117   2 99435475 99435833 359 - 0.134 0.332 1.659
ENSG00000135945 E047 16.8599051 0.0553621768 0.7990909399 0.895742023 2 99435834 99435836 3 - 1.194 1.216 0.078
ENSG00000135945 E048 30.4706283 0.0016228718 0.0445243222 0.166424266 2 99435837 99435941 105 - 1.467 1.346 -0.418
ENSG00000135945 E049 1.0301373 0.0313621164 0.6634465838   2 99436647 99436791 145 - 0.237 0.333 0.668
ENSG00000135945 E050 2.6366600 0.0092579975 0.6037407552 0.772636168 2 99438574 99438600 27 - 0.477 0.588 0.523
ENSG00000135945 E051 65.4922703 0.0008327990 0.2760085488 0.516077300 2 99438601 99438817 217 - 1.764 1.739 -0.081
ENSG00000135945 E052 114.6700909 0.0003989828 0.7081241705 0.840581230 2 99438818 99439310 493 - 1.993 2.011 0.058
ENSG00000135945 E053 57.9668018 0.0007081674 0.2695169198 0.509349787 2 99442317 99442469 153 - 1.714 1.686 -0.095
ENSG00000135945 E054 54.0363435 0.0012309233 0.0657996514 0.215718519 2 99449336 99449504 169 - 1.694 1.620 -0.253
ENSG00000135945 E055 7.6462700 0.0052097906 0.2356766436 0.471611555 2 99451345 99451501 157 - 0.833 1.007 0.658
ENSG00000135945 E056 32.5684087 0.0021847267 0.5774652063 0.755414453 2 99462496 99462622 127 - 1.450 1.519 0.238
ENSG00000135945 E057 0.0000000       2 99462623 99462788 166 -      
ENSG00000135945 E058 18.7592756 0.0045650898 0.0640257085 0.211716676 2 99464922 99464985 64 - 1.185 1.378 0.675
ENSG00000135945 E059 17.1390626 0.0020878269 0.0454944196 0.168793050 2 99489817 99490035 219 - 1.142 1.346 0.719