ENSG00000135924

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000336576 ENSG00000135924 HEK293_OSMI2_6hA HEK293_TMG_6hB DNAJB2 protein_coding protein_coding 21.68991 34.3023 11.09097 4.86967 0.719019 -1.62804 4.6535988 9.297541 1.2389159 1.368517 0.1676092 -2.8977238 0.1965625 0.27056667 0.11226667 -0.15830000 0.0005830843 0.0005830843 FALSE TRUE
ENST00000392086 ENSG00000135924 HEK293_OSMI2_6hA HEK293_TMG_6hB DNAJB2 protein_coding protein_coding 21.68991 34.3023 11.09097 4.86967 0.719019 -1.62804 10.6304103 20.631821 5.5858241 5.251150 0.2375913 -1.8831477 0.4886292 0.57903333 0.50506667 -0.07396667 0.8335893068 0.0005830843 FALSE TRUE
ENST00000463463 ENSG00000135924 HEK293_OSMI2_6hA HEK293_TMG_6hB DNAJB2 protein_coding processed_transcript 21.68991 34.3023 11.09097 4.86967 0.719019 -1.62804 0.4582613 0.000000 0.9641664 0.000000 0.5387877 6.6060963 0.0331625 0.00000000 0.08196667 0.08196667 0.1389163429 0.0005830843   FALSE
ENST00000472019 ENSG00000135924 HEK293_OSMI2_6hA HEK293_TMG_6hB DNAJB2 protein_coding retained_intron 21.68991 34.3023 11.09097 4.86967 0.719019 -1.62804 3.6041680 2.032136 1.7042045 1.129159 0.3365121 -0.2525398 0.1500125 0.06946667 0.15676667 0.08730000 0.7181253438 0.0005830843   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135924 E001 2.947739 0.1067642859 5.247262e-01 0.7182803704 2 219279342 219279343 2 + 0.377 0.520 0.742
ENSG00000135924 E002 3.889732 0.1387348547 6.605218e-01 0.8102904435 2 219279344 219279347 4 + 0.487 0.601 0.530
ENSG00000135924 E003 5.317583 0.0062880051 7.396180e-01 0.8601668595 2 219279348 219279365 18 + 0.651 0.703 0.218
ENSG00000135924 E004 6.746223 0.0324694776 9.634138e-01 0.9836556136 2 219279366 219279366 1 + 0.769 0.777 0.031
ENSG00000135924 E005 7.745933 0.0235010742 9.149069e-01 0.9596045462 2 219279367 219279368 2 + 0.817 0.835 0.070
ENSG00000135924 E006 28.969667 0.0030933745 2.786720e-02 0.1209057017 2 219279369 219279449 81 + 1.194 1.398 0.714
ENSG00000135924 E007 27.223594 0.0013441613 2.537630e-03 0.0205771868 2 219279450 219279464 15 + 1.084 1.378 1.038
ENSG00000135924 E008 41.498092 0.0015202786 7.151004e-02 0.2278475987 2 219279465 219279515 51 + 1.404 1.537 0.457
ENSG00000135924 E009 31.440284 0.0147655443 5.266505e-01 0.7195920136 2 219279516 219279516 1 + 1.341 1.408 0.231
ENSG00000135924 E010 31.137042 0.0170423702 5.792572e-01 0.7566491078 2 219279517 219279518 2 + 1.341 1.402 0.213
ENSG00000135924 E011 1.651950 0.6252084801 8.744328e-01 0.9384317795 2 219279519 219279587 69 + 0.387 0.342 -0.266
ENSG00000135924 E012 2.800757 0.5330178580 4.264717e-01 0.6483742070 2 219279588 219279687 100 + 0.663 0.446 -1.007
ENSG00000135924 E013 3.508370 0.3284876201 3.926978e-01 0.6225786310 2 219279688 219279797 110 + 0.720 0.524 -0.860
ENSG00000135924 E014 88.795507 0.0005962599 6.057987e-01 0.7740725486 2 219279798 219279898 101 + 1.818 1.838 0.070
ENSG00000135924 E015 1.053790 0.0384847612 2.474669e-02   2 219279899 219279905 7 + 0.577 0.161 -2.630
ENSG00000135924 E016 1.196425 0.1413182545 1.839505e-02   2 219279906 219279919 14 + 0.646 0.160 -2.943
ENSG00000135924 E017 2.118727 0.1329350000 1.327881e-02 0.0715638193 2 219279920 219279975 56 + 0.810 0.277 -2.616
ENSG00000135924 E018 1.225544 0.0960272036 2.374346e-01   2 219279976 219280059 84 + 0.488 0.242 -1.479
ENSG00000135924 E019 2.123385 0.1587740145 3.552601e-01 0.5919533365 2 219280060 219280170 111 + 0.577 0.373 -1.028
ENSG00000135924 E020 80.686648 0.0010500276 9.583123e-01 0.9810383039 2 219280578 219280612 35 + 1.794 1.792 -0.008
ENSG00000135924 E021 109.007101 0.0004054754 5.677239e-01 0.7485855255 2 219280613 219280687 75 + 1.948 1.919 -0.098
ENSG00000135924 E022 5.318795 0.0505323063 1.673301e-02 0.0844874187 2 219280688 219280839 152 + 1.002 0.601 -1.595
ENSG00000135924 E023 22.636340 0.2055619414 1.145943e-01 0.3068052281 2 219280840 219281717 878 + 1.505 1.183 -1.123
ENSG00000135924 E024 102.019713 0.0004729515 6.218730e-01 0.7850578070 2 219281718 219281771 54 + 1.920 1.893 -0.090
ENSG00000135924 E025 81.881635 0.0005429899 7.072542e-01 0.8400808085 2 219281939 219281959 21 + 1.794 1.808 0.049
ENSG00000135924 E026 144.447180 0.0010073702 7.689135e-01 0.8779613536 2 219281960 219282061 102 + 2.061 2.046 -0.051
ENSG00000135924 E027 3.512251 0.2420621225 9.729199e-02 0.2770404930 2 219282062 219282478 417 + 0.857 0.459 -1.723
ENSG00000135924 E028 2.958093 0.1680447226 6.848273e-01 0.8258564906 2 219282479 219282697 219 + 0.582 0.479 -0.486
ENSG00000135924 E029 1.226022 0.5868776602 1.000000e+00   2 219282698 219282741 44 + 0.230 0.289 0.438
ENSG00000135924 E030 2.107367 0.1199030340 2.975166e-01 0.5380424807 2 219282742 219282836 95 + 0.581 0.348 -1.195
ENSG00000135924 E031 167.036829 0.0002673453 5.303110e-01 0.7222769976 2 219282837 219282929 93 + 2.131 2.105 -0.087
ENSG00000135924 E032 6.331673 0.0894774345 2.745328e-02 0.1196601365 2 219282930 219283063 134 + 1.062 0.652 -1.594
ENSG00000135924 E033 5.230343 0.0792524247 7.748146e-02 0.2398326952 2 219283064 219283132 69 + 0.938 0.609 -1.324
ENSG00000135924 E034 177.934608 0.0002691946 1.150039e-02 0.0643905182 2 219283133 219283235 103 + 2.207 2.121 -0.288
ENSG00000135924 E035 2.374113 0.3867792967 1.152359e-01 0.3078546418 2 219283236 219283418 183 + 0.803 0.317 -2.316
ENSG00000135924 E036 161.355348 0.0002636400 1.048739e-02 0.0602055397 2 219283419 219283489 71 + 2.168 2.076 -0.305
ENSG00000135924 E037 226.548368 0.0005585413 2.641471e-02 0.1165183430 2 219284632 219284835 204 + 2.298 2.228 -0.234
ENSG00000135924 E038 93.515186 0.0005501793 1.871237e-03 0.0162041234 2 219284836 219285262 427 + 1.726 1.880 0.519
ENSG00000135924 E039 119.538537 0.0013780317 2.851425e-05 0.0005164649 2 219285263 219285776 514 + 1.794 1.990 0.660
ENSG00000135924 E040 27.752303 0.0014971102 2.740180e-02 0.1195001635 2 219285777 219285800 24 + 1.173 1.375 0.708
ENSG00000135924 E041 62.460225 0.0007504427 2.519536e-03 0.0204597729 2 219285801 219285986 186 + 1.528 1.713 0.632
ENSG00000135924 E042 248.497979 0.0002683272 7.869358e-01 0.8887161601 2 219285987 219286265 279 + 2.276 2.279 0.010
ENSG00000135924 E043 425.181796 0.0006307718 2.324144e-01 0.4679840980 2 219286266 219286898 633 + 2.494 2.517 0.075