ENSG00000135709

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000538274 ENSG00000135709 HEK293_OSMI2_6hA HEK293_TMG_6hB KIAA0513 protein_coding protein_coding 3.45907 5.088829 2.344472 0.5775449 0.03288618 -1.114762 2.4891921 4.1690995 1.3591830 0.3983422 0.05425145 -1.60987677 0.7235708 0.8232 0.5802000 -0.2430000 0.002668437 0.002668437 FALSE TRUE
ENST00000567328 ENSG00000135709 HEK293_OSMI2_6hA HEK293_TMG_6hB KIAA0513 protein_coding protein_coding 3.45907 5.088829 2.344472 0.5775449 0.03288618 -1.114762 0.7918115 0.8669334 0.8580596 0.2091560 0.03280639 -0.01467325 0.2276958 0.1650 0.3657333 0.2007333 0.020647795 0.002668437 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135709 E001 2.8516175 0.0082434682 4.157245e-01 6.403300e-01 16 85027782 85027793 12 + 0.429 0.576 0.715
ENSG00000135709 E002 3.3328439 0.0074593519 5.431023e-01 7.312629e-01 16 85027794 85027794 1 + 0.512 0.617 0.480
ENSG00000135709 E003 9.4272606 0.0030998017 1.744557e-01 3.964591e-01 16 85027795 85027851 57 + 0.821 0.995 0.657
ENSG00000135709 E004 8.7261843 0.0033467020 2.831004e-01 5.235545e-01 16 85027852 85027858 7 + 0.821 0.961 0.531
ENSG00000135709 E005 0.2735028 0.0260107835 4.167571e-01   16 85063212 85063670 459 + 0.194 0.074 -1.607
ENSG00000135709 E006 59.6854760 0.0009702617 4.362562e-03 3.116419e-02 16 85066900 85067400 501 + 1.822 1.682 -0.472
ENSG00000135709 E007 29.8832899 0.0011483514 2.948512e-02 1.258306e-01 16 85071783 85071882 100 + 1.534 1.385 -0.512
ENSG00000135709 E008 24.8424377 0.0118033249 1.079849e-02 6.151733e-02 16 85072925 85072998 74 + 1.498 1.273 -0.777
ENSG00000135709 E009 26.1710113 0.0023473401 1.912753e-02 9.289271e-02 16 85075844 85075914 71 + 1.489 1.314 -0.604
ENSG00000135709 E010 40.6232960 0.0009795548 2.518936e-01 4.899419e-01 16 85077425 85077632 208 + 1.607 1.537 -0.237
ENSG00000135709 E011 12.5281487 0.0028874341 9.025315e-01 9.531276e-01 16 85078415 85078418 4 + 1.088 1.075 -0.047
ENSG00000135709 E012 16.9926860 0.0025515835 4.162884e-01 6.408323e-01 16 85078419 85078455 37 + 1.252 1.178 -0.264
ENSG00000135709 E013 25.3699720 0.0014543623 7.659398e-01 8.761919e-01 16 85078925 85079003 79 + 1.339 1.362 0.080
ENSG00000135709 E014 18.9606076 0.0024773019 2.683761e-03 2.147907e-02 16 85079004 85079754 751 + 1.411 1.161 -0.875
ENSG00000135709 E015 19.9153667 0.0020649067 8.558651e-01 9.281526e-01 16 85081315 85081392 78 + 1.252 1.268 0.056
ENSG00000135709 E016 0.0000000       16 85082564 85082593 30 +      
ENSG00000135709 E017 19.2800873 0.0023281309 9.935043e-02 2.806059e-01 16 85086644 85086724 81 + 1.126 1.281 0.548
ENSG00000135709 E018 0.0000000       16 85086914 85087071 158 +      
ENSG00000135709 E019 19.7598135 0.0018004363 2.197196e-01 4.531080e-01 16 85087072 85087166 95 + 1.328 1.223 -0.366
ENSG00000135709 E020 453.1185127 0.0002336921 1.483361e-07 5.287759e-06 16 85088276 85094230 5955 + 2.536 2.607 0.234