ENSG00000135677

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000258145 ENSG00000135677 HEK293_OSMI2_6hA HEK293_TMG_6hB GNS protein_coding protein_coding 26.80159 20.60663 33.30116 4.033189 1.311441 0.6921972 24.545394 19.9158728 30.401662 3.92480874 0.9377138 0.6099817 0.91743333 0.965533333 0.91356667 -0.05196667 0.02172085 0.02172085 FALSE  
ENST00000540196 ENSG00000135677 HEK293_OSMI2_6hA HEK293_TMG_6hB GNS protein_coding protein_coding 26.80159 20.60663 33.30116 4.033189 1.311441 0.6921972 1.050127 0.1449994 2.515471 0.09694367 0.3178112 4.0262183 0.03236667 0.009866667 0.07506667 0.06520000 0.07293972 0.02172085 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135677 E001 1.1789786 0.0140327963 1.226186e-01   12 64713445 64713448 4 - 0.187 0.467 1.844
ENSG00000135677 E002 1996.4997458 0.0005729908 1.050043e-21 3.746248e-19 12 64713449 64716461 3013 - 3.241 3.356 0.382
ENSG00000135677 E003 122.8694709 0.0004327525 2.355023e-01 4.714172e-01 12 64716462 64716474 13 - 2.061 2.118 0.191
ENSG00000135677 E004 206.8070317 0.0003476185 8.534175e-01 9.268416e-01 12 64716475 64716608 134 - 2.298 2.321 0.078
ENSG00000135677 E005 251.4953320 0.0002086279 1.489913e-02 7.770691e-02 12 64716609 64716819 211 - 2.361 2.434 0.245
ENSG00000135677 E006 97.3411672 0.0004378668 4.833907e-03 3.371288e-02 12 64720022 64720038 17 - 1.931 2.051 0.403
ENSG00000135677 E007 101.5738474 0.0004592716 3.137383e-03 2.423029e-02 12 64720039 64720050 12 - 1.948 2.071 0.413
ENSG00000135677 E008 196.7318166 0.0003215208 4.661258e-01 6.773532e-01 12 64720051 64720182 132 - 2.272 2.309 0.123
ENSG00000135677 E009 204.0928680 0.0014763647 8.142044e-03 4.998392e-02 12 64721595 64721705 111 - 2.327 2.264 -0.211
ENSG00000135677 E010 0.9714428 0.0153787590 6.978990e-01   12 64722833 64723005 173 - 0.257 0.335 0.524
ENSG00000135677 E011 219.1465698 0.0020065026 2.356144e-03 1.941801e-02 12 64723006 64723113 108 - 2.364 2.286 -0.262
ENSG00000135677 E012 218.2539121 0.0004486525 3.296985e-07 1.072818e-05 12 64728956 64729057 102 - 2.377 2.263 -0.383
ENSG00000135677 E013 1.2928691 0.0136968632 7.242488e-02   12 64729058 64729218 161 - 0.460 0.142 -2.283
ENSG00000135677 E014 211.6638795 0.0003107193 9.143270e-05 1.386301e-03 12 64737004 64737107 104 - 2.352 2.270 -0.275
ENSG00000135677 E015 130.5191171 0.0003059712 2.652882e-03 2.129012e-02 12 64739381 64739403 23 - 2.141 2.063 -0.262
ENSG00000135677 E016 184.5067401 0.0002660925 3.060285e-05 5.491407e-04 12 64739404 64739499 96 - 2.298 2.204 -0.316
ENSG00000135677 E017 180.1409305 0.0019999833 3.513503e-04 4.252841e-03 12 64740606 64740688 83 - 2.292 2.189 -0.347
ENSG00000135677 E018 216.2725799 0.0002643913 4.378904e-05 7.434899e-04 12 64743141 64743308 168 - 2.363 2.278 -0.282
ENSG00000135677 E019 132.6519566 0.0015888532 5.327307e-02 1.873898e-01 12 64744809 64744907 99 - 2.140 2.087 -0.179
ENSG00000135677 E020 106.2878689 0.0004771594 2.494642e-04 3.214078e-03 12 64745659 64745724 66 - 2.066 1.952 -0.383
ENSG00000135677 E021 0.3641499 0.0275363265 8.150333e-01   12 64745725 64746238 514 - 0.104 0.142 0.525
ENSG00000135677 E022 197.1304085 0.0007069511 3.926291e-09 2.014425e-07 12 64747712 64747918 207 - 2.346 2.196 -0.498
ENSG00000135677 E023 106.3855762 0.0009110096 2.244769e-04 2.941782e-03 12 64752698 64752757 60 - 2.070 1.950 -0.402
ENSG00000135677 E024 6.8217974 0.0074090177 1.323327e-02 7.138408e-02 12 64752758 64752844 87 - 0.995 0.687 -1.204
ENSG00000135677 E025 0.0000000       12 64752845 64752971 127 -      
ENSG00000135677 E026 0.0000000       12 64756667 64756762 96 -      
ENSG00000135677 E027 159.8642894 0.0009301458 7.541979e-01 8.692535e-01 12 64759085 64759431 347 - 2.188 2.217 0.097