ENSG00000135632

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000389501 ENSG00000135632 HEK293_OSMI2_6hA HEK293_TMG_6hB SMYD5 protein_coding protein_coding 43.50134 59.32865 25.71788 9.399362 0.950677 -1.20564 38.211322 54.389692 20.915675 10.3925497 0.9148604 -1.378324 0.85322500 0.90480000 0.8129000 -0.0919 0.37604060 0.03632695 FALSE TRUE
ENST00000477038 ENSG00000135632 HEK293_OSMI2_6hA HEK293_TMG_6hB SMYD5 protein_coding retained_intron 43.50134 59.32865 25.71788 9.399362 0.950677 -1.20564 2.170742 1.345589 2.705828 0.2969435 0.4052122 1.002472 0.06457917 0.02606667 0.1062667 0.0802 0.03632695 0.03632695 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135632 E001 0.5283420 0.3410931588 2.282401e-01   2 73214222 73214237 16 + 0.325 0.077 -2.526
ENSG00000135632 E002 0.7900783 0.1078054955 4.942873e-01   2 73214238 73214244 7 + 0.322 0.195 -0.951
ENSG00000135632 E003 29.8400989 0.0041741267 3.983819e-01 0.6269603277 2 73214245 73214266 22 + 1.368 1.447 0.274
ENSG00000135632 E004 101.9588120 0.0004057474 6.753366e-01 0.8197657160 2 73214267 73214362 96 + 1.934 1.967 0.111
ENSG00000135632 E005 2.8684411 0.2166040329 3.155867e-01 0.5555888976 2 73214567 73214589 23 + 0.683 0.474 -0.952
ENSG00000135632 E006 5.9186268 0.1437712299 1.545589e-01 0.3688275797 2 73214590 73214705 116 + 0.988 0.689 -1.165
ENSG00000135632 E007 5.4427497 0.2393485630 4.131899e-01 0.6385108703 2 73214706 73214749 44 + 0.905 0.693 -0.836
ENSG00000135632 E008 183.8408365 0.0002583835 9.867271e-01 0.9952246596 2 73218861 73218969 109 + 2.199 2.215 0.051
ENSG00000135632 E009 3.9443363 0.2471932755 2.724688e-01 0.5123114953 2 73219917 73219951 35 + 0.805 0.575 -0.965
ENSG00000135632 E010 11.6780675 0.1046395800 3.010018e-01 0.5414595323 2 73219952 73220050 99 + 1.176 1.002 -0.631
ENSG00000135632 E011 132.5183130 0.0004073275 1.501518e-01 0.3625912789 2 73220051 73220069 19 + 2.097 2.063 -0.113
ENSG00000135632 E012 255.8090762 0.0010248098 2.612596e-01 0.5002975400 2 73220070 73220190 121 + 2.368 2.351 -0.055
ENSG00000135632 E013 285.3046870 0.0002615860 2.666432e-02 0.1172596397 2 73220661 73220782 122 + 2.429 2.392 -0.121
ENSG00000135632 E014 147.1352130 0.0003848943 2.170369e-02 0.1015955829 2 73221165 73221175 11 + 2.156 2.096 -0.201
ENSG00000135632 E015 224.2838674 0.0004722662 7.270715e-02 0.2301934420 2 73221176 73221234 59 + 2.319 2.284 -0.116
ENSG00000135632 E016 326.3691974 0.0034167726 5.597490e-01 0.7430954591 2 73221826 73221930 105 + 2.462 2.452 -0.034
ENSG00000135632 E017 249.9519473 0.0002881138 6.856769e-03 0.0439758723 2 73222755 73222817 63 + 2.380 2.327 -0.178
ENSG00000135632 E018 279.1426924 0.0001886270 7.286260e-04 0.0076841238 2 73223036 73223106 71 + 2.436 2.373 -0.212
ENSG00000135632 E019 5.2139327 0.0217948451 9.183729e-04 0.0092492490 2 73223107 73223167 61 + 1.033 0.566 -1.868
ENSG00000135632 E020 321.9891385 0.0002109902 1.154296e-01 0.3081928885 2 73223426 73223532 107 + 2.468 2.449 -0.064
ENSG00000135632 E021 221.2156121 0.0003263944 7.640313e-01 0.8750685970 2 73223947 73224003 57 + 2.273 2.298 0.082
ENSG00000135632 E022 277.6295403 0.0002014801 5.011787e-01 0.7016263322 2 73224866 73224960 95 + 2.365 2.398 0.108
ENSG00000135632 E023 5.3312107 0.1288189417 8.750598e-01 0.9387216929 2 73225222 73225630 409 + 0.768 0.742 -0.105
ENSG00000135632 E024 231.7573635 0.0004120447 1.118446e-02 0.0631295378 2 73225631 73225701 71 + 2.246 2.334 0.292
ENSG00000135632 E025 1459.3952823 0.0016228377 1.417105e-05 0.0002835595 2 73225796 73227221 1426 + 3.040 3.126 0.286