ENSG00000135540

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343505 ENSG00000135540 HEK293_OSMI2_6hA HEK293_TMG_6hB NHSL1 protein_coding protein_coding 2.688524 3.502005 2.655107 0.7774347 0.1547904 -0.3981014 1.62928135 2.5465573 1.6126779 0.63442309 0.01619066 -0.6558257 0.61549583 0.72780000 0.61100000 -0.11680000 7.334277e-01 7.016184e-06 FALSE TRUE
ENST00000427025 ENSG00000135540 HEK293_OSMI2_6hA HEK293_TMG_6hB NHSL1 protein_coding protein_coding 2.688524 3.502005 2.655107 0.7774347 0.1547904 -0.3981014 0.14429975 0.2462524 0.1051204 0.11612036 0.02887545 -1.1544221 0.05532500 0.06816667 0.03856667 -0.02960000 8.433419e-01 7.016184e-06 FALSE TRUE
ENST00000491526 ENSG00000135540 HEK293_OSMI2_6hA HEK293_TMG_6hB NHSL1 protein_coding protein_coding 2.688524 3.502005 2.655107 0.7774347 0.1547904 -0.3981014 0.29426971 0.3052406 0.0000000 0.30524057 0.00000000 -4.9783813 0.09637500 0.06633333 0.00000000 -0.06633333 9.038858e-01 7.016184e-06 FALSE FALSE
MSTRG.29032.3 ENSG00000135540 HEK293_OSMI2_6hA HEK293_TMG_6hB NHSL1 protein_coding   2.688524 3.502005 2.655107 0.7774347 0.1547904 -0.3981014 0.13931553 0.1618643 0.1538211 0.04718408 0.15382113 -0.0691484 0.05448750 0.05906667 0.06476667 0.00570000 7.950842e-01 7.016184e-06 FALSE TRUE
MSTRG.29032.4 ENSG00000135540 HEK293_OSMI2_6hA HEK293_TMG_6hB NHSL1 protein_coding   2.688524 3.502005 2.655107 0.7774347 0.1547904 -0.3981014 0.06205432 0.0000000 0.2423359 0.00000000 0.24233590 4.6572736 0.02310000 0.00000000 0.08326667 0.08326667 8.288900e-01 7.016184e-06 FALSE FALSE
MSTRG.29032.5 ENSG00000135540 HEK293_OSMI2_6hA HEK293_TMG_6hB NHSL1 protein_coding   2.688524 3.502005 2.655107 0.7774347 0.1547904 -0.3981014 0.15007278 0.0000000 0.3587204 0.00000000 0.08110577 5.2044554 0.05432917 0.00000000 0.13570000 0.13570000 7.016184e-06 7.016184e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135540 E001 145.1606754 0.0031278648 1.120343e-05 0.0002313869 6 138422043 138423966 1924 - 2.043 2.210 0.561
ENSG00000135540 E002 66.3714587 0.0045734882 2.973757e-05 0.0005358220 6 138423967 138424666 700 - 1.660 1.888 0.774
ENSG00000135540 E003 28.9009767 0.0359392776 5.886318e-01 0.7627751857 6 138424667 138424816 150 - 1.431 1.482 0.173
ENSG00000135540 E004 26.2752444 0.0073237914 4.351212e-01 0.6550675771 6 138429711 138429843 133 - 1.459 1.411 -0.165
ENSG00000135540 E005 191.7742120 0.0004409397 1.673393e-02 0.0844895717 6 138430393 138433507 3115 - 2.300 2.262 -0.129
ENSG00000135540 E006 24.0894115 0.0022774548 5.137394e-02 0.1830090203 6 138433508 138433680 173 - 1.461 1.334 -0.439
ENSG00000135540 E007 14.1799090 0.0029619420 3.872531e-01 0.6183314038 6 138441983 138442114 132 - 1.209 1.144 -0.229
ENSG00000135540 E008 1.3936670 0.0583950419 5.373490e-01 0.7272344588 6 138446790 138447000 211 - 0.422 0.307 -0.673
ENSG00000135540 E009 17.8909711 0.0155863308 1.431359e-01 0.3521894716 6 138447001 138447064 64 - 1.334 1.199 -0.476
ENSG00000135540 E010 17.0055115 0.0024828784 9.236081e-02 0.2681194875 6 138447065 138447123 59 - 1.315 1.190 -0.443
ENSG00000135540 E011 14.6042601 0.0101982108 8.476956e-02 0.2542712351 6 138447124 138447193 70 - 1.272 1.118 -0.545
ENSG00000135540 E012 0.0000000       6 138470541 138470594 54 -      
ENSG00000135540 E013 17.7902950 0.0077367486 3.333824e-03 0.0253894800 6 138473306 138473433 128 - 1.401 1.165 -0.831
ENSG00000135540 E014 13.3683953 0.0027245403 4.631046e-03 0.0326412452 6 138496219 138496371 153 - 1.288 1.047 -0.860
ENSG00000135540 E015 1.8923396 0.0442224583 6.688091e-02 0.2180608333 6 138499233 138499494 262 - 0.629 0.305 -1.677
ENSG00000135540 E016 0.0000000       6 138512279 138512495 217 -      
ENSG00000135540 E017 0.0000000       6 138545623 138545711 89 -      
ENSG00000135540 E018 0.0000000       6 138571530 138571698 169 -      
ENSG00000135540 E019 0.6709767 0.0311053046 1.392881e-01   6 138571699 138571709 11 - 0.348 0.099 -2.271
ENSG00000135540 E020 2.8357534 0.0680882537 5.576884e-01 0.7416121820 6 138571710 138572061 352 - 0.633 0.519 -0.517
ENSG00000135540 E021 0.8569551 0.0184865957 9.110736e-01   6 138572062 138572385 324 - 0.260 0.246 -0.101
ENSG00000135540 E022 1.3030199 0.0136968632 4.866561e-01   6 138572386 138572540 155 - 0.421 0.305 -0.687
ENSG00000135540 E023 4.3537402 0.0117771575 7.559335e-01 0.8701790217 6 138692476 138692571 96 - 0.671 0.727 0.234