ENSG00000135476

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257934 ENSG00000135476 HEK293_OSMI2_6hA HEK293_TMG_6hB ESPL1 protein_coding protein_coding 33.4473 51.2851 24.57451 5.074952 0.2610307 -1.061072 17.070155 28.729185 15.756355 2.6758718 0.58680106 -0.8661700 0.5235292 0.56150000 0.6408333 0.07933333 4.906589e-01 1.206149e-09 FALSE TRUE
ENST00000549154 ENSG00000135476 HEK293_OSMI2_6hA HEK293_TMG_6hB ESPL1 protein_coding retained_intron 33.4473 51.2851 24.57451 5.074952 0.2610307 -1.061072 3.447937 4.476754 2.648351 1.0873443 0.07600709 -0.7551405 0.1056000 0.09226667 0.1078000 0.01553333 8.658700e-01 1.206149e-09 FALSE TRUE
MSTRG.7351.1 ENSG00000135476 HEK293_OSMI2_6hA HEK293_TMG_6hB ESPL1 protein_coding   33.4473 51.2851 24.57451 5.074952 0.2610307 -1.061072 3.348198 5.145932 3.015655 0.2960246 0.15360925 -0.7689858 0.1035458 0.10123333 0.1228333 0.02160000 6.844165e-01 1.206149e-09 TRUE TRUE
MSTRG.7351.4 ENSG00000135476 HEK293_OSMI2_6hA HEK293_TMG_6hB ESPL1 protein_coding   33.4473 51.2851 24.57451 5.074952 0.2610307 -1.061072 4.262458 6.519961 0.000000 2.1658316 0.00000000 -9.3509307 0.1078917 0.12153333 0.0000000 -0.12153333 1.206149e-09 1.206149e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135476 E001 0.3751086 0.0274424043 6.100260e-01   12 53268261 53268262 2 + 0.001 0.147 8.000
ENSG00000135476 E002 16.2876792 0.0021148119 2.594771e-01 4.984354e-01 12 53268263 53268298 36 + 1.258 1.160 -0.347
ENSG00000135476 E003 69.2213262 0.0008577105 3.379429e-03 2.563666e-02 12 53268299 53268332 34 + 1.888 1.756 -0.445
ENSG00000135476 E004 74.1164626 0.0006574584 2.514193e-03 2.042224e-02 12 53268333 53268334 2 + 1.916 1.786 -0.440
ENSG00000135476 E005 108.1307887 0.0003881327 4.982627e-04 5.658072e-03 12 53268335 53268377 43 + 2.076 1.953 -0.414
ENSG00000135476 E006 25.5375590 0.0180750204 6.414841e-02 2.120050e-01 12 53268378 53268406 29 + 1.495 1.317 -0.615
ENSG00000135476 E007 165.0586306 0.0003277357 1.514462e-08 6.820221e-07 12 53268755 53268847 93 + 2.286 2.123 -0.547
ENSG00000135476 E008 14.5226122 0.0022591032 2.171053e-02 1.016220e-01 12 53268848 53268928 81 + 1.282 1.074 -0.743
ENSG00000135476 E009 401.5372402 0.0003948982 7.702776e-10 4.589855e-08 12 53269024 53269525 502 + 2.643 2.519 -0.412
ENSG00000135476 E010 221.5840685 0.0002323359 2.599708e-04 3.321974e-03 12 53269526 53269701 176 + 2.366 2.275 -0.302
ENSG00000135476 E011 379.5606225 0.0010611947 1.210294e-04 1.750283e-03 12 53269702 53270085 384 + 2.598 2.507 -0.302
ENSG00000135476 E012 216.6483019 0.0003550804 5.190160e-06 1.189806e-04 12 53270378 53270482 105 + 2.374 2.257 -0.391
ENSG00000135476 E013 236.8405889 0.0006582798 2.649004e-04 3.374301e-03 12 53270678 53270798 121 + 2.397 2.301 -0.318
ENSG00000135476 E014 262.6555716 0.0003006536 1.785905e-03 1.560523e-02 12 53272721 53272857 137 + 2.426 2.354 -0.241
ENSG00000135476 E015 21.7661772 0.1359769064 6.199760e-01 7.837992e-01 12 53274081 53274466 386 + 1.326 1.303 -0.082
ENSG00000135476 E016 271.1858819 0.0002190893 4.777682e-01 6.856316e-01 12 53274817 53275010 194 + 2.398 2.386 -0.042
ENSG00000135476 E017 317.0137034 0.0002295074 7.463848e-01 8.646219e-01 12 53276620 53276859 240 + 2.457 2.454 -0.010
ENSG00000135476 E018 246.3662458 0.0002074559 7.463912e-03 4.685237e-02 12 53277083 53277227 145 + 2.389 2.327 -0.208
ENSG00000135476 E019 250.8471465 0.0003403329 2.870533e-02 1.234758e-01 12 53277470 53277608 139 + 2.389 2.337 -0.173
ENSG00000135476 E020 217.1921800 0.0002209497 2.553755e-01 4.940210e-01 12 53277821 53277960 140 + 2.312 2.286 -0.086
ENSG00000135476 E021 0.7794795 0.0171013947 6.994613e-01   12 53279705 53279731 27 + 0.181 0.256 0.632
ENSG00000135476 E022 236.6651560 0.0011274822 1.822547e-02 8.977301e-02 12 53279732 53279866 135 + 2.377 2.311 -0.218
ENSG00000135476 E023 265.0790219 0.0002114631 1.030587e-06 2.915985e-05 12 53281507 53281626 120 + 2.456 2.345 -0.373
ENSG00000135476 E024 0.9006742 0.2896316868 6.828184e-01   12 53282250 53282263 14 + 0.310 0.212 -0.729
ENSG00000135476 E025 300.8278632 0.0002698980 2.879242e-04 3.609105e-03 12 53282264 53282435 172 + 2.488 2.409 -0.264
ENSG00000135476 E026 271.7755239 0.0001857977 2.778724e-01 5.179469e-01 12 53283129 53283257 129 + 2.404 2.382 -0.073
ENSG00000135476 E027 268.2257667 0.0031313630 5.893467e-01 7.632495e-01 12 53283382 53283538 157 + 2.366 2.389 0.076
ENSG00000135476 E028 206.4145175 0.0002898295 1.994341e-01 4.290262e-01 12 53284058 53284167 110 + 2.242 2.283 0.137
ENSG00000135476 E029 875.0101231 0.0002079592 6.234036e-02 2.080254e-01 12 53285924 53286912 989 + 2.873 2.904 0.102
ENSG00000135476 E030 22.1030204 0.0927355299 9.889730e-01 9.962946e-01 12 53287256 53287880 625 + 1.314 1.316 0.005
ENSG00000135476 E031 9.9202486 0.0329622938 3.531164e-01 5.901131e-01 12 53287881 53287971 91 + 0.888 1.023 0.505
ENSG00000135476 E032 599.1818842 0.0002697796 4.935021e-02 1.780308e-01 12 53287972 53288341 370 + 2.704 2.743 0.129
ENSG00000135476 E033 361.0303557 0.0053976712 3.445390e-01 5.821054e-01 12 53288538 53288699 162 + 2.480 2.521 0.137
ENSG00000135476 E034 17.0459189 0.1842814589 3.074782e-01 5.480943e-01 12 53288700 53289089 390 + 1.313 1.162 -0.532
ENSG00000135476 E035 441.5218285 0.0002148384 1.443574e-01 3.539284e-01 12 53289090 53289303 214 + 2.576 2.609 0.110
ENSG00000135476 E036 419.9314366 0.0001788075 7.482210e-05 1.173812e-03 12 53289404 53289594 191 + 2.515 2.600 0.282
ENSG00000135476 E037 122.8416991 0.0705918999 1.861813e-01 4.120398e-01 12 53289595 53290084 490 + 2.142 2.000 -0.477
ENSG00000135476 E038 359.5892257 0.0013847298 4.541011e-04 5.257845e-03 12 53290085 53290212 128 + 2.433 2.532 0.330
ENSG00000135476 E039 49.5595150 0.0635853715 4.356321e-01 6.554649e-01 12 53290213 53290346 134 + 1.713 1.631 -0.278
ENSG00000135476 E040 344.9536323 0.0020458839 5.884174e-03 3.917162e-02 12 53290347 53290469 123 + 2.425 2.514 0.296
ENSG00000135476 E041 353.8010114 0.0110666463 8.478490e-02 2.542768e-01 12 53290841 53290996 156 + 2.429 2.525 0.320
ENSG00000135476 E042 434.7576053 0.0001902699 5.746010e-07 1.754098e-05 12 53291690 53291860 171 + 2.514 2.619 0.351
ENSG00000135476 E043 371.4510288 0.0002801654 1.888895e-04 2.550714e-03 12 53291984 53292088 105 + 2.459 2.545 0.287
ENSG00000135476 E044 401.3926497 0.0010634962 1.408026e-03 1.295807e-02 12 53292278 53292393 116 + 2.493 2.577 0.278
ENSG00000135476 E045 283.7009020 0.0034636267 3.245702e-03 2.487566e-02 12 53292574 53292657 84 + 2.320 2.433 0.377
ENSG00000135476 E046 294.0973072 0.0004518084 8.561360e-08 3.231642e-06 12 53292806 53292970 165 + 2.313 2.456 0.476
ENSG00000135476 E047 358.2228368 0.0002269782 6.720640e-08 2.601594e-06 12 53293273 53293638 366 + 2.415 2.541 0.419