ENSG00000135148

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257604 ENSG00000135148 HEK293_OSMI2_6hA HEK293_TMG_6hB TRAFD1 protein_coding protein_coding 44.75259 46.29381 36.0947 5.198442 1.214289 -0.3589444 5.21049 5.574572 3.929870 0.6455891 0.3639769 -0.5032986 0.11907083 0.12256667 0.1089667 -0.0136000 0.9245382300 0.0003466192 FALSE TRUE
ENST00000412615 ENSG00000135148 HEK293_OSMI2_6hA HEK293_TMG_6hB TRAFD1 protein_coding protein_coding 44.75259 46.29381 36.0947 5.198442 1.214289 -0.3589444 32.49802 36.456753 23.065646 4.4425279 1.2410550 -0.6602107 0.71529167 0.78566667 0.6382000 -0.1474667 0.0003466192 0.0003466192 FALSE TRUE
ENST00000550051 ENSG00000135148 HEK293_OSMI2_6hA HEK293_TMG_6hB TRAFD1 protein_coding retained_intron 44.75259 46.29381 36.0947 5.198442 1.214289 -0.3589444 2.64212 1.309442 4.271974 0.4858344 0.1732616 1.6983481 0.06452083 0.02826667 0.1188667 0.0906000 0.0048820924 0.0003466192 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135148 E001 0.5705883 5.258402e-01 8.278548e-01   12 112125515 112125516 2 + 0.152 0.231 0.743
ENSG00000135148 E002 3.5162879 1.368354e-02 1.959464e-02 9.453468e-02 12 112125517 112125537 21 + 0.832 0.479 -1.528
ENSG00000135148 E003 6.0240324 4.532183e-03 9.780277e-03 5.717274e-02 12 112125538 112125544 7 + 1.004 0.679 -1.268
ENSG00000135148 E004 11.4138087 3.092592e-03 1.172760e-04 1.706319e-03 12 112125545 112125553 9 + 1.274 0.892 -1.387
ENSG00000135148 E005 15.1336859 2.865348e-03 1.026228e-04 1.528345e-03 12 112125554 112125556 3 + 1.376 1.034 -1.213
ENSG00000135148 E006 19.2447601 3.889096e-03 7.004246e-06 1.539185e-04 12 112125557 112125559 3 + 1.483 1.117 -1.282
ENSG00000135148 E007 25.0951127 1.488472e-03 9.047972e-07 2.603887e-05 12 112125560 112125562 3 + 1.582 1.240 -1.185
ENSG00000135148 E008 135.4917156 4.798261e-04 8.888253e-02 2.616994e-01 12 112125563 112125618 56 + 2.146 2.092 -0.181
ENSG00000135148 E009 70.1671805 1.027791e-02 1.610157e-01 3.783298e-01 12 112125619 112126084 466 + 1.777 1.870 0.312
ENSG00000135148 E010 38.2824566 3.791024e-03 3.110946e-01 5.515008e-01 12 112126085 112126149 65 + 1.618 1.554 -0.220
ENSG00000135148 E011 1.4645377 2.505599e-02 1.183038e-01 3.130758e-01 12 112130470 112130510 41 + 0.150 0.443 2.101
ENSG00000135148 E012 202.1860616 5.111251e-04 7.976763e-03 4.920409e-02 12 112130511 112130569 59 + 2.328 2.258 -0.234
ENSG00000135148 E013 0.1426347 3.159954e-02 3.023990e-01   12 112133140 112133320 181 + 0.150 0.000 -9.548
ENSG00000135148 E014 311.5367051 2.051426e-04 2.356278e-03 1.941817e-02 12 112134738 112134873 136 + 2.510 2.448 -0.209
ENSG00000135148 E015 221.8588994 3.363648e-04 2.714252e-03 2.165371e-02 12 112135013 112135066 54 + 2.369 2.295 -0.248
ENSG00000135148 E016 1.7606636 1.301629e-02 2.759216e-01 5.159929e-01 12 112140119 112140205 87 + 0.541 0.354 -0.975
ENSG00000135148 E017 323.8720553 1.676967e-04 4.031744e-02 1.555249e-01 12 112140819 112140980 162 + 2.515 2.473 -0.138
ENSG00000135148 E018 419.4596892 1.883492e-04 1.093288e-01 2.979988e-01 12 112140981 112141224 244 + 2.618 2.590 -0.094
ENSG00000135148 E019 9.7167922 3.411450e-03 6.481451e-08 2.521648e-06 12 112141225 112141491 267 + 1.283 0.702 -2.174
ENSG00000135148 E020 6.0577610 6.599949e-02 1.437930e-01 3.530645e-01 12 112141953 112142088 136 + 0.949 0.730 -0.853
ENSG00000135148 E021 352.1383603 8.172183e-04 6.522340e-01 8.048830e-01 12 112142089 112142295 207 + 2.534 2.526 -0.027
ENSG00000135148 E022 259.4877302 2.276415e-04 2.163252e-01 4.490800e-01 12 112145586 112145662 77 + 2.411 2.383 -0.094
ENSG00000135148 E023 460.1331353 2.025335e-04 9.439505e-01 9.743296e-01 12 112148074 112148304 231 + 2.641 2.643 0.006
ENSG00000135148 E024 148.1416648 1.873549e-03 1.930135e-01 4.208096e-01 12 112149751 112149756 6 + 2.182 2.138 -0.147
ENSG00000135148 E025 314.1549634 1.925800e-03 8.924928e-01 9.478455e-01 12 112149757 112149871 115 + 2.477 2.485 0.024
ENSG00000135148 E026 524.6521166 4.915209e-04 2.017030e-03 1.717799e-02 12 112151801 112152140 340 + 2.665 2.723 0.193
ENSG00000135148 E027 238.2160707 3.923040e-04 6.319110e-04 6.856623e-03 12 112152427 112152499 73 + 2.297 2.385 0.293
ENSG00000135148 E028 2.8559628 7.015379e-01 2.707971e-01 5.107291e-01 12 112152500 112152504 5 + 0.151 0.700 3.274
ENSG00000135148 E029 152.8832895 3.142384e-04 5.147277e-02 1.832319e-01 12 112152735 112152740 6 + 2.125 2.184 0.200
ENSG00000135148 E030 784.0454657 9.971976e-05 9.505099e-16 1.598202e-13 12 112152741 112153604 864 + 2.804 2.909 0.349