ENSG00000135116

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257572 ENSG00000135116 HEK293_OSMI2_6hA HEK293_TMG_6hB HRK protein_coding protein_coding 3.187739 2.733129 2.577412 0.2427522 0.8133051 -0.08431299 2.2282000 2.5279729 1.6869208 0.18296862 0.47691172 -0.58075744 0.73855417 0.92786667 0.6750667 -0.2528000 0.003636977 0.003636977 FALSE TRUE
ENST00000552092 ENSG00000135116 HEK293_OSMI2_6hA HEK293_TMG_6hB HRK protein_coding processed_transcript 3.187739 2.733129 2.577412 0.2427522 0.8133051 -0.08431299 0.1171317 0.1157901 0.1189605 0.06773977 0.08760434 0.03591078 0.04464167 0.03943333 0.1020333 0.0626000 1.000000000 0.003636977 FALSE FALSE
MSTRG.8098.5 ENSG00000135116 HEK293_OSMI2_6hA HEK293_TMG_6hB HRK protein_coding   3.187739 2.733129 2.577412 0.2427522 0.8133051 -0.08431299 0.5811542 0.0000000 0.7523444 0.00000000 0.44127598 6.25237093 0.14726667 0.00000000 0.2177667 0.2177667 0.126914890 0.003636977   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000135116 E001 208.3362821 0.005033200 0.007197362 0.04556996 12 116856144 116859790 3647 - 2.234 2.326 0.308
ENSG00000135116 E002 8.9283472 0.062026203 0.742908199 0.86227445 12 116859791 116859889 99 - 0.993 0.979 -0.055
ENSG00000135116 E003 8.9572437 0.004247646 0.192613130 0.42028602 12 116859890 116859976 87 - 1.070 0.937 -0.488
ENSG00000135116 E004 25.0716930 0.001746883 0.251394113 0.48946733 12 116859977 116860281 305 - 1.445 1.376 -0.238
ENSG00000135116 E005 8.4025586 0.018692193 0.158777641 0.37492833 12 116860282 116860313 32 - 1.067 0.900 -0.622
ENSG00000135116 E006 42.4244864 0.001081804 0.001032973 0.01016236 12 116860314 116861023 710 - 1.720 1.560 -0.542
ENSG00000135116 E007 13.2131249 0.008984380 0.104825841 0.29032942 12 116861024 116861126 103 - 1.220 1.071 -0.536
ENSG00000135116 E008 14.8837958 0.004773452 0.406300956 0.63326798 12 116861127 116861276 150 - 1.221 1.155 -0.235
ENSG00000135116 E009 14.2590565 0.003515339 0.359128881 0.59515339 12 116861277 116861332 56 - 1.102 1.200 0.352
ENSG00000135116 E010 20.9396694 0.002393164 0.554628638 0.73953616 12 116861333 116861466 134 - 1.287 1.345 0.203
ENSG00000135116 E011 0.8102147 0.112364937 0.237784414   12 116867649 116867791 143 - 0.369 0.166 -1.522
ENSG00000135116 E012 0.0000000       12 116869477 116869655 179 -      
ENSG00000135116 E013 0.9662812 0.524335114 0.006446495   12 116876691 116876800 110 - 0.569 0.000 -13.272
ENSG00000135116 E014 0.2617363 1.468299463 1.000000000   12 116878458 116878544 87 - 0.000 0.152 9.222
ENSG00000135116 E015 1.9798958 2.088119931 0.808306158 0.90119997 12 116879073 116879617 545 - 0.527 0.409 -0.596
ENSG00000135116 E016 14.1567946 0.018823413 0.027113187 0.11862535 12 116880976 116881441 466 - 0.956 1.240 1.028