ENSG00000134352

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000336909 ENSG00000134352 HEK293_OSMI2_6hA HEK293_TMG_6hB IL6ST protein_coding protein_coding 9.140614 1.0659 21.53961 0.04944005 1.810197 4.324046 0.55893459 0.2222280 1.72271737 0.17517787 0.68936266 2.899423 0.19882917 0.2142333 0.084600000 -0.1296333 0.927754136 0.001503901 FALSE TRUE
ENST00000381287 ENSG00000134352 HEK293_OSMI2_6hA HEK293_TMG_6hB IL6ST protein_coding protein_coding 9.140614 1.0659 21.53961 0.04944005 1.810197 4.324046 0.06229836 0.1699906 0.04338025 0.16999063 0.04338025 -1.753544 0.04843333 0.1465667 0.002166667 -0.1444000 0.705729569 0.001503901 FALSE TRUE
ENST00000381294 ENSG00000134352 HEK293_OSMI2_6hA HEK293_TMG_6hB IL6ST protein_coding protein_coding 9.140614 1.0659 21.53961 0.04944005 1.810197 4.324046 0.81025035 0.0000000 2.89950811 0.00000000 0.37009725 8.184631 0.04638333 0.0000000 0.138566667 0.1385667 0.002352720 0.001503901 FALSE TRUE
ENST00000502326 ENSG00000134352 HEK293_OSMI2_6hA HEK293_TMG_6hB IL6ST protein_coding protein_coding 9.140614 1.0659 21.53961 0.04944005 1.810197 4.324046 1.69444534 0.0000000 3.42048395 0.00000000 0.68973871 8.422268 0.10090833 0.0000000 0.160433333 0.1604333 0.001503901 0.001503901 FALSE TRUE
ENST00000523039 ENSG00000134352 HEK293_OSMI2_6hA HEK293_TMG_6hB IL6ST protein_coding retained_intron 9.140614 1.0659 21.53961 0.04944005 1.810197 4.324046 0.02097444 0.1677955 0.00000000 0.16779550 0.00000000 -4.152147 0.02114167 0.1691333 0.000000000 -0.1691333 0.488414023 0.001503901   FALSE
ENST00000583149 ENSG00000134352 HEK293_OSMI2_6hA HEK293_TMG_6hB IL6ST protein_coding retained_intron 9.140614 1.0659 21.53961 0.04944005 1.810197 4.324046 1.53897443 0.1029783 4.53069325 0.10297829 1.47127153 5.328795 0.09201667 0.0888000 0.202833333 0.1140333 0.524711742 0.001503901 FALSE FALSE
ENST00000651614 ENSG00000134352 HEK293_OSMI2_6hA HEK293_TMG_6hB IL6ST protein_coding nonsense_mediated_decay 9.140614 1.0659 21.53961 0.04944005 1.810197 4.324046 4.04180424 0.4029078 8.88813644 0.04004346 1.20505448 4.429612 0.46634583 0.3813000 0.410000000 0.0287000 0.967007722 0.001503901 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000134352 E001 0.0000000       5 55935095 55935098 4 -      
ENSG00000134352 E002 0.0000000       5 55935099 55935124 26 -      
ENSG00000134352 E003 7.1062310 0.2537267801 1.998658e-01 0.4295112824 5 55935125 55936649 1525 - 0.537 1.091 2.215
ENSG00000134352 E004 604.8656680 0.0744837859 5.273738e-04 0.0059271306 5 55936650 55940965 4316 - 2.353 2.831 1.594
ENSG00000134352 E005 29.0434335 0.0055679397 3.047581e-01 0.5453514350 5 55940966 55941027 62 - 1.110 1.176 0.234
ENSG00000134352 E006 25.1255425 0.1483641201 3.095437e-01 0.5500272846 5 55941028 55941081 54 - 1.053 0.936 -0.432
ENSG00000134352 E007 93.7331377 1.3627327371 5.906446e-01 0.7641563695 5 55941082 55941522 441 - 1.574 1.824 0.847
ENSG00000134352 E008 55.9372257 0.9432735785 3.744313e-01 0.6077009892 5 55941523 55941693 171 - 1.346 1.733 1.325
ENSG00000134352 E009 38.4216877 0.2872574793 2.912077e-01 0.5317894779 5 55941694 55941764 71 - 1.192 1.576 1.333
ENSG00000134352 E010 41.4568156 0.0304628222 6.177997e-01 0.7823000109 5 55941765 55941819 55 - 1.244 1.361 0.410
ENSG00000134352 E011 54.8518931 0.0060251634 6.183429e-01 0.7826609731 5 55942670 55942751 82 - 1.357 1.527 0.587
ENSG00000134352 E012 51.4189752 0.0235918826 2.123682e-01 0.4445165109 5 55947493 55947560 68 - 1.333 1.411 0.270
ENSG00000134352 E013 32.2046136 0.0340831387 7.816201e-01 0.8855986815 5 55947561 55947564 4 - 1.135 1.325 0.671
ENSG00000134352 E014 41.5550880 0.0524484389 6.100432e-01 0.7769097542 5 55947565 55947589 25 - 1.240 1.408 0.586
ENSG00000134352 E015 0.0000000       5 55947590 55947594 5 -      
ENSG00000134352 E016 0.8807956 0.0172671820 4.091023e-03   5 55948102 55948897 796 - 0.079 0.753 4.538
ENSG00000134352 E017 0.4646582 0.0217681645 6.333101e-03   5 55948898 55948982 85 - 0.028 0.614 5.537
ENSG00000134352 E018 61.4977723 0.0547324619 2.543711e-01 0.4928473351 5 55951464 55951604 141 - 1.407 1.480 0.250
ENSG00000134352 E019 0.3641499 0.0322028964 6.034773e-02   5 55951605 55951637 33 - 0.028 0.408 4.542
ENSG00000134352 E020 62.7059014 0.0317243388 4.176936e-02 0.1593162700 5 55951929 55952075 147 - 1.420 1.384 -0.125
ENSG00000134352 E021 55.3027224 0.0345390872 2.406936e-03 0.0197300345 5 55952250 55952351 102 - 1.374 1.129 -0.861
ENSG00000134352 E022 66.6296568 0.0355042249 5.964718e-03 0.0395512223 5 55954810 55954992 183 - 1.450 1.294 -0.542
ENSG00000134352 E023 0.5657866 0.0198837603 1.703490e-02   5 55955980 55956024 45 - 0.055 0.613 4.536
ENSG00000134352 E024 86.6687460 0.0443257004 2.690144e-02 0.1180090271 5 55956025 55956235 211 - 1.557 1.501 -0.195
ENSG00000134352 E025 63.1263184 0.0644502745 1.376132e-01 0.3438720896 5 55957209 55957291 83 - 1.422 1.429 0.025
ENSG00000134352 E026 0.0000000       5 55959626 55959671 46 -      
ENSG00000134352 E027 67.1048617 0.0354310342 6.914362e-03 0.0442383211 5 55960402 55960561 160 - 1.453 1.294 -0.551
ENSG00000134352 E028 58.4062386 0.0302018791 3.279086e-02 0.1354735944 5 55963352 55963506 155 - 1.392 1.327 -0.223
ENSG00000134352 E029 39.2430112 0.0345500994 3.537732e-02 0.1425588742 5 55964146 55964208 63 - 1.230 1.126 -0.371
ENSG00000134352 E030 49.3474531 0.0216554802 2.698653e-02 0.1182594128 5 55964209 55964312 104 - 1.324 1.256 -0.239
ENSG00000134352 E031 56.4413968 0.0007496625 1.949907e-02 0.0941940245 5 55968276 55968396 121 - 1.382 1.357 -0.087
ENSG00000134352 E032 68.8086122 0.0259141751 2.655007e-05 0.0004853926 5 55969550 55969759 210 - 1.469 1.075 -1.388
ENSG00000134352 E033 42.7073003 0.0384653120 5.748235e-04 0.0063520466 5 55969760 55969855 96 - 1.271 0.859 -1.504
ENSG00000134352 E034 33.9474685 0.0102525931 3.100167e-04 0.0038317294 5 55976215 55976293 79 - 1.178 0.754 -1.592
ENSG00000134352 E035 0.0000000       5 55976294 55976314 21 -      
ENSG00000134352 E036 24.0609923 0.0269635019 3.441081e-02 0.1399897903 5 55982724 55982811 88 - 1.035 0.858 -0.662
ENSG00000134352 E037 13.5234881 0.0610869741 4.650436e-01 0.6766002966 5 55994784 55995022 239 - 0.805 0.858 0.204