ENSG00000134278

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000410092 ENSG00000134278 HEK293_OSMI2_6hA HEK293_TMG_6hB SPIRE1 protein_coding protein_coding 8.059124 4.729631 11.6218 1.258025 1.210761 1.295228 5.2786781 3.920288 7.290546 1.005123 0.3977306 0.8933693 0.71815417 0.8357667 0.63493333 -0.20083333 0.01750318 0.01750318 FALSE TRUE
ENST00000588236 ENSG00000134278 HEK293_OSMI2_6hA HEK293_TMG_6hB SPIRE1 protein_coding retained_intron 8.059124 4.729631 11.6218 1.258025 1.210761 1.295228 0.5704791 0.000000 1.120265 0.000000 0.6389801 6.8205170 0.04994583 0.0000000 0.08906667 0.08906667 0.41094157 0.01750318 FALSE FALSE
MSTRG.15521.6 ENSG00000134278 HEK293_OSMI2_6hA HEK293_TMG_6hB SPIRE1 protein_coding   8.059124 4.729631 11.6218 1.258025 1.210761 1.295228 0.8381788 0.000000 1.308937 0.000000 0.6654236 7.0432322 0.07253750 0.0000000 0.12163333 0.12163333 0.45240269 0.01750318 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000134278 E001 0.0000000       18 12446512 12446512 1 -      
ENSG00000134278 E002 446.3181653 0.0224830229 0.010433871 0.06000580 18 12446513 12449503 2991 - 2.562 2.735 0.576
ENSG00000134278 E003 22.1826425 0.0020685513 0.810332672 0.90241550 18 12449504 12449505 2 - 1.328 1.392 0.223
ENSG00000134278 E004 22.0417434 0.0189247037 0.971883163 0.98785442 18 12449506 12449508 3 - 1.330 1.381 0.175
ENSG00000134278 E005 39.9322615 0.0038434845 0.741982914 0.86164268 18 12449509 12449572 64 - 1.572 1.644 0.247
ENSG00000134278 E006 45.4096464 0.0010079163 0.463436642 0.67543914 18 12449573 12449665 93 - 1.616 1.704 0.297
ENSG00000134278 E007 25.2859294 0.0029941427 0.797458324 0.89486677 18 12449666 12449667 2 - 1.386 1.415 0.101
ENSG00000134278 E008 58.1245029 0.0078368809 0.249318814 0.48711538 18 12449668 12449809 142 - 1.757 1.734 -0.077
ENSG00000134278 E009 46.3439307 0.0011989171 0.468557953 0.67905715 18 12449810 12449896 87 - 1.652 1.659 0.026
ENSG00000134278 E010 58.2315467 0.0007333815 0.648304322 0.80238423 18 12452255 12452391 137 - 1.746 1.771 0.084
ENSG00000134278 E011 1.0539778 0.0161340178 0.658090155   18 12452392 12452443 52 - 0.335 0.277 -0.383
ENSG00000134278 E012 35.0684014 0.0013208519 0.231046194 0.46639040 18 12452485 12452512 28 - 1.550 1.521 -0.100
ENSG00000134278 E013 3.9355954 0.0070091692 0.022389409 0.10386922 18 12452513 12452610 98 - 0.523 0.886 1.520
ENSG00000134278 E014 44.4839788 0.0011454635 0.153364618 0.36713191 18 12453068 12453138 71 - 1.653 1.619 -0.116
ENSG00000134278 E015 55.5049998 0.0096191322 0.977043580 0.99028052 18 12454346 12454483 138 - 1.715 1.758 0.147
ENSG00000134278 E016 0.4952057 0.3228105385 1.000000000   18 12454484 12454506 23 - 0.167 0.161 -0.055
ENSG00000134278 E017 3.4418177 0.0072568474 0.225393242 0.45980416 18 12459755 12459928 174 - 0.552 0.771 0.937
ENSG00000134278 E018 53.2274373 0.0079070031 0.452490782 0.66751531 18 12463351 12463493 143 - 1.715 1.711 -0.012
ENSG00000134278 E019 34.8164229 0.0117049978 0.272303578 0.51221359 18 12464868 12464958 91 - 1.547 1.513 -0.119
ENSG00000134278 E020 53.9727187 0.0079074995 0.219288099 0.45255885 18 12479699 12479871 173 - 1.732 1.701 -0.105
ENSG00000134278 E021 1.3855550 0.0726036895 0.895612647   18 12485959 12486000 42 - 0.378 0.369 -0.055
ENSG00000134278 E022 35.2958667 0.0063644954 0.200477816 0.43027992 18 12493072 12493138 67 - 1.555 1.504 -0.177
ENSG00000134278 E023 35.7269597 0.0009639104 0.845284936 0.92227202 18 12493139 12493201 63 - 1.536 1.570 0.118
ENSG00000134278 E024 43.1379861 0.0011686399 0.526568818 0.71955002 18 12496016 12496102 87 - 1.621 1.633 0.040
ENSG00000134278 E025 29.8007043 0.0016225826 0.085286752 0.25515404 18 12506477 12506495 19 - 1.492 1.422 -0.242
ENSG00000134278 E026 66.9862729 0.0007083057 0.002306936 0.01909672 18 12506496 12506641 146 - 1.844 1.751 -0.313
ENSG00000134278 E027 48.6759370 0.0009399626 0.011023832 0.06246850 18 12512454 12512512 59 - 1.704 1.615 -0.304
ENSG00000134278 E028 32.7098289 0.0011898774 0.020192113 0.09656403 18 12512513 12512531 19 - 1.541 1.437 -0.359
ENSG00000134278 E029 0.0000000       18 12516071 12516225 155 -      
ENSG00000134278 E030 27.6966997 0.0014851250 0.024523030 0.11075407 18 12535476 12535477 2 - 1.472 1.359 -0.390
ENSG00000134278 E031 60.0873984 0.0007657182 0.027385304 0.11944356 18 12535478 12535601 124 - 1.785 1.726 -0.198
ENSG00000134278 E032 58.7344251 0.0053591114 0.282138746 0.52250434 18 12546674 12546904 231 - 1.758 1.745 -0.045
ENSG00000134278 E033 0.0000000       18 12588376 12588419 44 -      
ENSG00000134278 E034 13.4180907 0.0183036697 0.925420631 0.96487198 18 12635062 12635096 35 - 1.110 1.174 0.227
ENSG00000134278 E035 0.0000000       18 12636324 12636340 17 -      
ENSG00000134278 E036 0.0000000       18 12647795 12647865 71 -      
ENSG00000134278 E037 0.0000000       18 12653001 12653085 85 -      
ENSG00000134278 E038 0.0000000       18 12653384 12653440 57 -      
ENSG00000134278 E039 0.0000000       18 12656575 12656650 76 -      
ENSG00000134278 E040 0.0000000       18 12656651 12656736 86 -      
ENSG00000134278 E041 17.3813016 0.0024468862 0.079830157 0.24448544 18 12657530 12658107 578 - 1.176 1.367 0.673