ENSG00000134198

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369515 ENSG00000134198 HEK293_OSMI2_6hA HEK293_TMG_6hB TSPAN2 protein_coding protein_coding 1.571382 0.4898281 1.887958 0.155497 0.2815379 1.924944 0.4399559 0.2408259 0.2795449 0.15254594 0.2795449 0.207101 0.2565542 0.4106667 0.1229333 -0.2877333 0.59147177 0.04817625 FALSE TRUE
ENST00000369516 ENSG00000134198 HEK293_OSMI2_6hA HEK293_TMG_6hB TSPAN2 protein_coding protein_coding 1.571382 0.4898281 1.887958 0.155497 0.2815379 1.924944 0.8880320 0.2490022 1.2116576 0.05367962 0.1073225 2.237804 0.6228208 0.5893333 0.6601333 0.0708000 0.98969197 0.04817625 FALSE TRUE
ENST00000433172 ENSG00000134198 HEK293_OSMI2_6hA HEK293_TMG_6hB TSPAN2 protein_coding protein_coding 1.571382 0.4898281 1.887958 0.155497 0.2815379 1.924944 0.2433943 0.0000000 0.3967558 0.00000000 0.1302042 5.346091 0.1206250 0.0000000 0.2169333 0.2169333 0.04817625 0.04817625 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000134198 E001 35.281009 0.002146932 0.02023986 0.09671067 1 115048011 115050355 2345 - 1.520 1.374 -0.504
ENSG00000134198 E002 6.341363 0.010508672 0.35394458 0.59078083 1 115050356 115050382 27 - 0.839 0.705 -0.538
ENSG00000134198 E003 6.863548 0.004550388 0.42084054 0.64432039 1 115050383 115050444 62 - 0.862 0.752 -0.432
ENSG00000134198 E004 11.454185 0.009955844 0.69523053 0.83241302 1 115050445 115050555 111 - 1.037 0.988 -0.177
ENSG00000134198 E005 13.331536 0.003615506 0.44730782 0.66372351 1 115053379 115053462 84 - 1.064 1.140 0.275
ENSG00000134198 E006 12.016184 0.003455230 0.07854526 0.24188139 1 115057537 115057608 72 - 0.998 1.175 0.639
ENSG00000134198 E007 0.000000       1 115058229 115058425 197 -      
ENSG00000134198 E008 13.306721 0.005320451 0.13788786 0.34427467 1 115058883 115058981 99 - 1.043 1.192 0.537
ENSG00000134198 E009 8.409028 0.004934303 0.82313155 0.90978032 1 115060464 115060538 75 - 0.902 0.932 0.114
ENSG00000134198 E010 6.897594 0.004490090 0.72766145 0.85282611 1 115062135 115062232 98 - 0.840 0.795 -0.176
ENSG00000134198 E011 5.169806 0.006462898 0.88781210 0.94546188 1 115072905 115073007 103 - 0.725 0.705 -0.080
ENSG00000134198 E012 4.132877 0.054634416 0.11616128 0.30944053 1 115089364 115089503 140 - 0.569 0.833 1.099