ENSG00000133302

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265140 ENSG00000133302 HEK293_OSMI2_6hA HEK293_TMG_6hB SLF1 protein_coding protein_coding 6.962772 1.50887 12.69865 0.285521 0.6644081 3.064741 3.74282219 0.78968462 7.7801195 0.02558450 0.21508368 3.2841424 0.48351667 0.56923333 0.61426667 0.04503333 0.9568769332 0.0003643789 FALSE TRUE
ENST00000475916 ENSG00000133302 HEK293_OSMI2_6hA HEK293_TMG_6hB SLF1 protein_coding retained_intron 6.962772 1.50887 12.69865 0.285521 0.6644081 3.064741 0.06140288 0.08269709 0.1229829 0.04276509 0.07334636 0.5206448 0.01386250 0.06150000 0.01033333 -0.05116667 0.6054632079 0.0003643789   FALSE
ENST00000508130 ENSG00000133302 HEK293_OSMI2_6hA HEK293_TMG_6hB SLF1 protein_coding nonsense_mediated_decay 6.962772 1.50887 12.69865 0.285521 0.6644081 3.064741 0.75332024 0.00000000 1.5357988 0.00000000 0.19970985 7.2722087 0.06457083 0.00000000 0.12200000 0.12200000 0.0003643789 0.0003643789 TRUE TRUE
MSTRG.26604.12 ENSG00000133302 HEK293_OSMI2_6hA HEK293_TMG_6hB SLF1 protein_coding   6.962772 1.50887 12.69865 0.285521 0.6644081 3.064741 0.55600606 0.07290137 0.7354302 0.07290137 0.22281891 3.1686056 0.07321667 0.04406667 0.05703333 0.01296667 0.8518108388 0.0003643789   FALSE
MSTRG.26604.13 ENSG00000133302 HEK293_OSMI2_6hA HEK293_TMG_6hB SLF1 protein_coding   6.962772 1.50887 12.69865 0.285521 0.6644081 3.064741 1.28214521 0.53575430 0.8873695 0.29373720 0.52521878 0.7174505 0.29432500 0.29613333 0.06750000 -0.22863333 0.7862401783 0.0003643789   FALSE
MSTRG.26604.6 ENSG00000133302 HEK293_OSMI2_6hA HEK293_TMG_6hB SLF1 protein_coding   6.962772 1.50887 12.69865 0.285521 0.6644081 3.064741 0.16906134 0.00000000 0.7033853 0.00000000 0.36570077 6.1566096 0.02270833 0.00000000 0.05396667 0.05396667 0.6572648725 0.0003643789 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000133302 E001 0.6434498 0.0196156836 1.0000000000   5 94618669 94618670 2 + 0.156 0.000 -9.050
ENSG00000133302 E002 1.4295343 0.0128005745 0.3783940629 0.610838917 5 94618671 94618677 7 + 0.295 0.000 -12.136
ENSG00000133302 E003 6.1982644 0.0064755363 0.0754049141 0.235679161 5 94618678 94618684 7 + 0.700 0.293 -2.059
ENSG00000133302 E004 6.3596801 0.0054208801 0.0679831905 0.220555879 5 94618685 94618686 2 + 0.709 0.293 -2.097
ENSG00000133302 E005 11.8500236 0.0030958804 0.0139383211 0.074095270 5 94618687 94618708 22 + 0.944 0.466 -2.018
ENSG00000133302 E006 21.6975752 0.0021282130 0.4428972811 0.660681831 5 94618709 94618765 57 + 1.159 1.064 -0.341
ENSG00000133302 E007 0.1614157 0.0334389149 0.6385103686   5 94620065 94620171 107 + 0.045 0.000 -9.049
ENSG00000133302 E008 31.2305936 0.0013664705 0.9640036417 0.983906342 5 94628811 94628881 71 + 1.302 1.306 0.015
ENSG00000133302 E009 26.8117758 0.0016584816 0.4025496505 0.630425892 5 94628882 94628924 43 + 1.252 1.160 -0.326
ENSG00000133302 E010 36.2669112 0.0012325231 0.8174534211 0.906624819 5 94629092 94629167 76 + 1.367 1.346 -0.074
ENSG00000133302 E011 0.1779838 0.0352079807 0.6388673456   5 94629664 94629720 57 + 0.045 0.000 -9.038
ENSG00000133302 E012 64.9289517 0.0006949429 0.6888446133 0.828335238 5 94630503 94630743 241 + 1.613 1.585 -0.094
ENSG00000133302 E013 52.8021669 0.0007752314 0.0242250257 0.109767244 5 94643273 94643435 163 + 1.538 1.345 -0.665
ENSG00000133302 E014 45.2430462 0.0008316358 0.0067682766 0.043554719 5 94649454 94649597 144 + 1.476 1.214 -0.909
ENSG00000133302 E015 45.6147742 0.0009343027 0.0224786443 0.104155161 5 94651702 94651845 144 + 1.476 1.263 -0.740
ENSG00000133302 E016 45.3977980 0.0011061845 0.5212087703 0.715703808 5 94653272 94653421 150 + 1.450 1.502 0.179
ENSG00000133302 E017 45.7571345 0.0026743958 0.5733169160 0.752533609 5 94654630 94654752 123 + 1.455 1.502 0.161
ENSG00000133302 E018 35.6383952 0.0010378878 0.7359622731 0.857924651 5 94662298 94662351 54 + 1.351 1.382 0.107
ENSG00000133302 E019 60.8014380 0.0007185644 0.7073278917 0.840115657 5 94663750 94663908 159 + 1.580 1.607 0.091
ENSG00000133302 E020 38.2661122 0.0079923955 0.9048128890 0.954366985 5 94665861 94665906 46 + 1.391 1.380 -0.038
ENSG00000133302 E021 53.5790447 0.0009028291 0.8098097188 0.902070893 5 94665907 94666024 118 + 1.533 1.515 -0.061
ENSG00000133302 E022 42.5458780 0.0009660967 0.2846499637 0.525193474 5 94670151 94670279 129 + 1.441 1.345 -0.331
ENSG00000133302 E023 56.8067753 0.0006529306 0.0920684167 0.267554241 5 94670843 94671008 166 + 1.565 1.431 -0.460
ENSG00000133302 E024 2.4099106 0.0105525012 0.2611394874 0.500174718 5 94678399 94678807 409 + 0.361 0.590 1.154
ENSG00000133302 E025 59.3710299 0.0007790535 0.7843522031 0.887143475 5 94678808 94678955 148 + 1.571 1.552 -0.067
ENSG00000133302 E026 55.5582998 0.0007170008 0.7256708770 0.851555054 5 94686573 94686718 146 + 1.537 1.563 0.090
ENSG00000133302 E027 59.6863293 0.0007006156 0.4589253595 0.672455908 5 94688506 94688669 164 + 1.565 1.617 0.177
ENSG00000133302 E028 53.2009337 0.0007275700 0.8270694021 0.912136942 5 94689473 94689606 134 + 1.523 1.540 0.057
ENSG00000133302 E029 1.5303323 0.0160487920 0.0619108556 0.207117137 5 94691195 94691563 369 + 0.217 0.590 2.153
ENSG00000133302 E030 49.3023622 0.0137125490 0.7382445041 0.859417027 5 94691564 94691656 93 + 1.487 1.515 0.097
ENSG00000133302 E031 51.4132522 0.0010833715 0.0930279896 0.269347227 5 94692074 94692185 112 + 1.494 1.617 0.419
ENSG00000133302 E032 45.5569258 0.0009529879 0.1764262202 0.399005072 5 94692186 94692256 71 + 1.446 1.552 0.361
ENSG00000133302 E033 191.6919377 0.0002826417 0.0252800745 0.113057648 5 94694831 94697621 2791 + 2.062 2.145 0.276
ENSG00000133302 E034 1.5533880 0.0123242597 0.3221806056 0.561609445 5 94697831 94697917 87 + 0.318 0.000 -12.267
ENSG00000133302 E035 2.3052705 0.0105801747 0.2178604376 0.450875639 5 94701695 94701771 77 + 0.341 0.590 1.278
ENSG00000133302 E036 1.6960226 0.0121505489 0.2760476905 0.516092034 5 94702010 94702027 18 + 0.340 0.000 -12.384
ENSG00000133302 E037 2.2241770 0.0107014350 0.6251118161 0.787390608 5 94702523 94702576 54 + 0.361 0.466 0.568
ENSG00000133302 E038 3.9914302 0.1661119695 0.0413731379 0.158287665 5 94708225 94708289 65 + 0.463 0.897 1.855
ENSG00000133302 E039 4.7304486 0.0309861087 0.0003355898 0.004095307 5 94714588 94714730 143 + 0.484 1.063 2.365
ENSG00000133302 E040 0.0000000       5 94739164 94739436 273 +