ENSG00000133114

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379151 ENSG00000133114 HEK293_OSMI2_6hA HEK293_TMG_6hB GPALPP1 protein_coding protein_coding 12.64274 4.074674 20.63823 0.1341614 0.5022192 2.337726 1.0735752 0.4357250 2.5365970 0.02608384 0.4359145 2.51434479 0.08795417 0.1072000 0.12406667 0.01686667 9.567253e-01 7.354798e-09 FALSE TRUE
ENST00000479068 ENSG00000133114 HEK293_OSMI2_6hA HEK293_TMG_6hB GPALPP1 protein_coding processed_transcript 12.64274 4.074674 20.63823 0.1341614 0.5022192 2.337726 0.4310056 0.3711168 0.3871720 0.19870514 0.2625864 0.05953071 0.05545417 0.0910000 0.01930000 -0.07170000 7.770126e-01 7.354798e-09 FALSE FALSE
ENST00000497558 ENSG00000133114 HEK293_OSMI2_6hA HEK293_TMG_6hB GPALPP1 protein_coding nonsense_mediated_decay 12.64274 4.074674 20.63823 0.1341614 0.5022192 2.337726 2.8023676 0.6031985 4.3806921 0.10443412 0.6544361 2.84002228 0.20265417 0.1467667 0.21106667 0.06430000 6.895678e-01 7.354798e-09 TRUE TRUE
ENST00000611650 ENSG00000133114 HEK293_OSMI2_6hA HEK293_TMG_6hB GPALPP1 protein_coding protein_coding 12.64274 4.074674 20.63823 0.1341614 0.5022192 2.337726 0.4962018 0.8427176 0.4175003 0.05686003 0.1134066 -0.99614275 0.08805833 0.2064333 0.02023333 -0.18620000 7.354798e-09 7.354798e-09   FALSE
MSTRG.8686.1 ENSG00000133114 HEK293_OSMI2_6hA HEK293_TMG_6hB GPALPP1 protein_coding   12.64274 4.074674 20.63823 0.1341614 0.5022192 2.337726 6.7113171 1.6285362 11.7875094 0.08255712 0.4014538 2.84800287 0.48237917 0.4005667 0.57096667 0.17040000 2.658944e-02 7.354798e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000133114 E001 0.6246688 0.0192103050 5.303047e-01   13 44989518 44989528 11 + 0.198 0.000 -10.314
ENSG00000133114 E002 0.6246688 0.0192103050 5.303047e-01   13 44989529 44989537 9 + 0.198 0.000 -11.312
ENSG00000133114 E003 0.7860845 0.0169313416 3.878253e-01   13 44989538 44989549 12 + 0.236 0.000 -11.619
ENSG00000133114 E004 6.7049652 0.0051171019 9.413622e-01 9.728808e-01 13 44989550 44989557 8 + 0.782 0.781 -0.002
ENSG00000133114 E005 13.3393949 0.0028610942 2.522137e-02 1.128791e-01 13 44989558 44989563 6 + 0.998 1.245 0.889
ENSG00000133114 E006 36.9416659 0.0027660267 6.092133e-02 2.048542e-01 13 44989564 44989614 51 + 1.429 1.577 0.508
ENSG00000133114 E007 37.1988903 0.0035656033 2.282343e-02 1.053961e-01 13 44989615 44989619 5 + 1.424 1.602 0.608
ENSG00000133114 E008 73.8932956 0.0009371522 6.649988e-02 2.171896e-01 13 44989620 44989742 123 + 1.730 1.838 0.363
ENSG00000133114 E009 10.8162800 0.0033796753 3.614536e-20 1.096128e-17 13 44989743 44990427 685 + 0.590 1.534 3.524
ENSG00000133114 E010 2.7184893 0.2301878005 1.456830e-02 7.649596e-02 13 45001703 45002040 338 + 0.305 0.885 2.714
ENSG00000133114 E011 37.5903044 0.0012611521 2.146839e-01 4.471596e-01 13 45004305 45004310 6 + 1.477 1.394 -0.286
ENSG00000133114 E012 88.7847863 0.0007527530 6.552316e-02 2.150732e-01 13 45004311 45004437 127 + 1.843 1.763 -0.268
ENSG00000133114 E013 0.6600180 0.0193874923 5.277883e-01   13 45005110 45005241 132 + 0.198 0.000 -11.314
ENSG00000133114 E014 99.8586607 0.0005041128 2.102004e-02 9.941043e-02 13 45006202 45006303 102 + 1.896 1.799 -0.325
ENSG00000133114 E015 98.7500171 0.0051175345 4.806344e-01 6.875140e-01 13 45008795 45008879 85 + 1.881 1.855 -0.088
ENSG00000133114 E016 125.8010686 0.0070926264 3.023409e-02 1.280410e-01 13 45014952 45015083 132 + 1.997 1.888 -0.365
ENSG00000133114 E017 136.1060446 0.0044481600 1.502962e-02 7.819775e-02 13 45015432 45015596 165 + 2.029 1.925 -0.350
ENSG00000133114 E018 103.7045666 0.0048630112 1.976078e-01 4.266039e-01 13 45020330 45020413 84 + 1.905 1.852 -0.177
ENSG00000133114 E019 48.5134731 0.0043243282 9.332366e-01 9.687778e-01 13 45020414 45020428 15 + 1.570 1.593 0.081
ENSG00000133114 E020 107.5822088 0.0005732919 7.089422e-01 8.411269e-01 13 45027785 45028003 219 + 1.914 1.913 -0.006
ENSG00000133114 E021 27.7492380 0.0151677974 4.818500e-01 6.883239e-01 13 45028004 45029238 1235 + 1.320 1.408 0.305
ENSG00000133114 E022 150.5718679 0.0009696246 3.851705e-02 1.509179e-01 13 45029239 45030435 1197 + 2.067 2.000 -0.227
ENSG00000133114 E023 42.6935233 0.0081960614 8.283821e-01 9.128614e-01 13 45030436 45031091 656 + 1.520 1.517 -0.008
ENSG00000133114 E024 9.1257705 0.5418208449 7.453628e-01 8.639195e-01 13 45031092 45031203 112 + 0.893 0.915 0.082
ENSG00000133114 E025 30.0532910 0.8140767392 5.375119e-01 7.273168e-01 13 45031204 45031665 462 + 1.358 1.440 0.284
ENSG00000133114 E026 45.7034841 0.9254239072 4.342100e-01 6.544174e-01 13 45031666 45032107 442 + 1.514 1.686 0.586
ENSG00000133114 E027 36.2217684 0.8253664004 2.897137e-01 5.302638e-01 13 45033236 45033813 578 + 1.384 1.680 1.011
ENSG00000133114 E028 9.1192718 0.1331433790 2.303813e-01 4.655351e-01 13 45033814 45036129 2316 + 0.818 1.152 1.243
ENSG00000133114 E029 8.5175189 0.0150981245 5.659082e-03 3.803397e-02 13 45036130 45037669 1540 + 0.772 1.147 1.407