ENSG00000132953

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000255305 ENSG00000132953 HEK293_OSMI2_6hA HEK293_TMG_6hB XPO4 protein_coding protein_coding 6.463726 1.75176 12.40059 0.481049 0.4194668 2.816483 3.45024724 1.27187286 6.067487 0.36282265 0.2890903 2.245222 0.61322917 0.71923333 0.4888667 -0.23036667 6.175435e-03 1.541753e-07 FALSE TRUE
MSTRG.8429.3 ENSG00000132953 HEK293_OSMI2_6hA HEK293_TMG_6hB XPO4 protein_coding   6.463726 1.75176 12.40059 0.481049 0.4194668 2.816483 0.01401073 0.06000452 0.000000 0.03897915 0.0000000 -2.807448 0.01115000 0.06323333 0.0000000 -0.06323333 2.706193e-01 1.541753e-07 FALSE TRUE
MSTRG.8429.4 ENSG00000132953 HEK293_OSMI2_6hA HEK293_TMG_6hB XPO4 protein_coding   6.463726 1.75176 12.40059 0.481049 0.4194668 2.816483 0.74690520 0.38229308 1.326241 0.13739908 0.1861322 1.768177 0.17073333 0.20076667 0.1082000 -0.09256667 2.923831e-01 1.541753e-07 FALSE TRUE
MSTRG.8429.5 ENSG00000132953 HEK293_OSMI2_6hA HEK293_TMG_6hB XPO4 protein_coding   6.463726 1.75176 12.40059 0.481049 0.4194668 2.816483 1.25986191 0.03758962 2.604946 0.03758962 0.3420255 5.779990 0.11490000 0.01680000 0.2088333 0.19203333 9.822700e-04 1.541753e-07 FALSE TRUE
MSTRG.8429.6 ENSG00000132953 HEK293_OSMI2_6hA HEK293_TMG_6hB XPO4 protein_coding   6.463726 1.75176 12.40059 0.481049 0.4194668 2.816483 0.99270071 0.00000000 2.401920 0.00000000 0.1799018 7.914038 0.09000417 0.00000000 0.1941000 0.19410000 1.541753e-07 1.541753e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000132953 E001 0.5775531 0.019387492 7.335699e-01   13 20777329 20777406 78 - 0.141 0.258 1.080
ENSG00000132953 E002 1.2131806 0.018073674 6.384059e-01   13 20777407 20777448 42 - 0.247 0.419 1.081
ENSG00000132953 E003 4.7988161 0.005752680 1.532833e-01 0.3670374984 13 20777449 20777791 343 - 0.581 0.919 1.376
ENSG00000132953 E004 316.4598654 1.169130196 2.079191e-01 0.4393142365 13 20777792 20781689 3898 - 2.222 2.741 1.731
ENSG00000132953 E005 259.7386362 1.662200694 5.023231e-01 0.7023961732 13 20781690 20783919 2230 - 2.203 2.494 0.971
ENSG00000132953 E006 35.1199269 0.001160702 1.278345e-01 0.3284201702 13 20786965 20787057 93 - 1.364 1.619 0.876
ENSG00000132953 E007 48.2821231 0.024000197 7.108380e-01 0.8423648156 13 20787481 20787598 118 - 1.514 1.647 0.454
ENSG00000132953 E008 49.5549891 0.026913570 2.082179e-01 0.4396887661 13 20788486 20788616 131 - 1.543 1.568 0.083
ENSG00000132953 E009 51.3041534 0.009673496 2.062316e-01 0.4372610750 13 20790462 20790580 119 - 1.556 1.610 0.186
ENSG00000132953 E010 59.1883684 0.016210542 4.034506e-01 0.6310991680 13 20796076 20796256 181 - 1.611 1.705 0.320
ENSG00000132953 E011 94.9183896 0.030361700 3.148590e-02 0.1317326772 13 20796764 20797057 294 - 1.831 1.801 -0.101
ENSG00000132953 E012 81.6499673 0.028656816 1.125670e-02 0.0634297909 13 20799165 20799339 175 - 1.771 1.689 -0.277
ENSG00000132953 E013 81.1364210 0.041902628 9.595270e-02 0.2746729860 13 20800156 20800325 170 - 1.760 1.753 -0.023
ENSG00000132953 E014 68.2316971 0.040076320 6.985454e-02 0.2245104424 13 20800831 20800990 160 - 1.690 1.645 -0.154
ENSG00000132953 E015 62.8518885 0.028457481 1.231591e-02 0.0677000298 13 20807457 20807634 178 - 1.662 1.560 -0.348
ENSG00000132953 E016 57.1692788 0.017444180 6.312514e-03 0.0412613079 13 20808436 20808581 146 - 1.621 1.524 -0.330
ENSG00000132953 E017 57.9269267 0.022450143 3.734166e-02 0.1478443415 13 20809083 20809225 143 - 1.618 1.590 -0.095
ENSG00000132953 E018 62.5730021 0.013452238 5.745038e-02 0.1970603877 13 20809791 20809967 177 - 1.646 1.651 0.015
ENSG00000132953 E019 58.6844105 0.019872050 4.250077e-02 0.1612365472 13 20821704 20821878 175 - 1.624 1.590 -0.115
ENSG00000132953 E020 54.1932565 0.012595155 4.720331e-05 0.0007917242 13 20822132 20822289 158 - 1.610 1.391 -0.752
ENSG00000132953 E021 51.5993345 0.028677323 3.698354e-02 0.1468554700 13 20827067 20827179 113 - 1.571 1.522 -0.167
ENSG00000132953 E022 52.6012688 0.032797872 1.347456e-01 0.3392973213 13 20842895 20843048 154 - 1.572 1.581 0.030
ENSG00000132953 E023 44.7868286 0.014797470 5.733301e-02 0.1967956151 13 20843770 20843886 117 - 1.507 1.492 -0.052
ENSG00000132953 E024 20.8030085 0.001643699 3.616028e-01 0.5969958525 13 20855627 20855633 7 - 1.182 1.237 0.193
ENSG00000132953 E025 46.3790115 0.015090695 2.784018e-02 0.1208411364 13 20855634 20855765 132 - 1.526 1.480 -0.159
ENSG00000132953 E026 48.3809374 0.014282933 5.979572e-03 0.0396265080 13 20862717 20862858 142 - 1.550 1.455 -0.326
ENSG00000132953 E027 7.3745139 0.012176448 3.778252e-01 0.6104057135 13 20862969 20863123 155 - 0.739 0.997 0.992
ENSG00000132953 E028 0.5008152 0.047416180 7.650006e-01   13 20863124 20863139 16 - 0.142 0.000 -8.963
ENSG00000132953 E029 0.0000000       13 20868413 20868595 183 -      
ENSG00000132953 E030 27.9878781 0.001805110 7.736761e-03 0.0480763253 13 20868596 20868701 106 - 1.322 1.216 -0.371
ENSG00000132953 E031 0.3206185 0.026179215 1.000000e+00   13 20898896 20898951 56 - 0.099 0.000 -8.375
ENSG00000132953 E032 15.7108559 0.055037781 2.084961e-01 0.4400624903 13 20902670 20903048 379 - 1.083 1.002 -0.298