ENSG00000132780

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000350030 ENSG00000132780 HEK293_OSMI2_6hA HEK293_TMG_6hB NASP protein_coding protein_coding 299.5766 225.2151 391.9066 22.48198 9.542234 0.7991794 41.88517 52.20889 26.93910 13.0231441 2.3830486 -0.9543348 0.14662917 0.2243000 0.06856667 -0.15573333 0.002410354 0.002410354 FALSE TRUE
ENST00000351223 ENSG00000132780 HEK293_OSMI2_6hA HEK293_TMG_6hB NASP protein_coding protein_coding 299.5766 225.2151 391.9066 22.48198 9.542234 0.7991794 66.13975 44.73289 81.97273 4.7248419 3.8984812 0.8736615 0.23339167 0.1987000 0.20923333 0.01053333 0.923034961 0.002410354 FALSE TRUE
ENST00000453748 ENSG00000132780 HEK293_OSMI2_6hA HEK293_TMG_6hB NASP protein_coding nonsense_mediated_decay 299.5766 225.2151 391.9066 22.48198 9.542234 0.7991794 18.74531 15.08015 16.35143 0.5631394 1.3304624 0.1166915 0.06346250 0.0690000 0.04190000 -0.02710000 0.328398062 0.002410354 FALSE TRUE
ENST00000464190 ENSG00000132780 HEK293_OSMI2_6hA HEK293_TMG_6hB NASP protein_coding retained_intron 299.5766 225.2151 391.9066 22.48198 9.542234 0.7991794 66.64404 36.87849 113.56086 3.8422500 2.7337906 1.6223500 0.20700833 0.1671000 0.29000000 0.12290000 0.062999944 0.002410354 FALSE TRUE
ENST00000525515 ENSG00000132780 HEK293_OSMI2_6hA HEK293_TMG_6hB NASP protein_coding protein_coding 299.5766 225.2151 391.9066 22.48198 9.542234 0.7991794 17.71228 11.22642 29.71239 1.1772189 2.7742964 1.4033678 0.05517917 0.0506000 0.07556667 0.02496667 0.332970150 0.002410354 FALSE FALSE
ENST00000528238 ENSG00000132780 HEK293_OSMI2_6hA HEK293_TMG_6hB NASP protein_coding protein_coding 299.5766 225.2151 391.9066 22.48198 9.542234 0.7991794 44.39269 32.39385 62.58949 4.1248412 0.9346052 0.9499854 0.14830833 0.1435333 0.15993333 0.01640000 0.797727002 0.002410354 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000132780 E001 4.0796329 7.778876e-01 3.283309e-01 5.673596e-01 1 45581985 45583319 1335 + 0.901 0.236 -3.264
ENSG00000132780 E002 1.1353746 4.806354e-01 2.041228e-01   1 45583320 45583845 526 + 0.114 0.489 2.800
ENSG00000132780 E003 1.7839860 2.656489e-01 9.180973e-02 2.670447e-01 1 45583846 45583912 67 + 0.203 0.616 2.389
ENSG00000132780 E004 0.9984221 3.298066e-01 8.722152e-01   1 45583913 45583987 75 + 0.287 0.304 0.113
ENSG00000132780 E005 0.6648197 5.295079e-01 5.948849e-01   1 45583988 45583988 1 + 0.289 0.125 -1.511
ENSG00000132780 E006 1.7165144 3.002188e-01 6.009446e-01 7.708274e-01 1 45583989 45584040 52 + 0.505 0.309 -1.084
ENSG00000132780 E007 1.7165144 3.002188e-01 6.009446e-01 7.708274e-01 1 45584041 45584041 1 + 0.505 0.309 -1.084
ENSG00000132780 E008 2.0087983 1.441401e-01 4.751722e-01 6.836670e-01 1 45584042 45584044 3 + 0.541 0.374 -0.859
ENSG00000132780 E009 53.4918256 7.624428e-04 4.957484e-02 1.785984e-01 1 45584045 45584057 13 + 1.775 1.690 -0.287
ENSG00000132780 E010 84.6689454 4.975646e-04 3.262764e-03 2.497261e-02 1 45584058 45584059 2 + 1.978 1.873 -0.352
ENSG00000132780 E011 100.6230449 7.041483e-04 9.527420e-06 2.009907e-04 1 45584060 45584060 1 + 2.073 1.919 -0.517
ENSG00000132780 E012 241.1473670 7.347891e-04 1.036210e-39 1.675926e-36 1 45584061 45584062 2 + 2.516 2.167 -1.165
ENSG00000132780 E013 383.9610475 6.118281e-04 2.292635e-25 1.211725e-22 1 45584063 45584064 2 + 2.676 2.458 -0.725
ENSG00000132780 E014 816.8719438 2.269606e-04 3.930338e-12 3.657325e-10 1 45584065 45584107 43 + 2.952 2.865 -0.291
ENSG00000132780 E015 1184.3194632 8.153375e-04 1.231409e-06 3.402565e-05 1 45584108 45584205 98 + 3.106 3.035 -0.237
ENSG00000132780 E016 3.3084739 1.563096e-01 9.993286e-01 1.000000e+00 1 45588651 45588948 298 + 0.606 0.629 0.097
ENSG00000132780 E017 1020.7080647 6.372347e-04 1.412595e-05 2.829309e-04 1 45591223 45591270 48 + 3.037 2.977 -0.197
ENSG00000132780 E018 3.1534704 1.128009e-01 6.773614e-01 8.210615e-01 1 45594694 45594773 80 + 0.574 0.674 0.436
ENSG00000132780 E019 33.6630284 1.137487e-03 3.489168e-01 5.863259e-01 1 45600385 45600457 73 + 1.559 1.513 -0.158
ENSG00000132780 E020 1406.9864770 1.524411e-03 1.608338e-04 2.228200e-03 1 45602255 45602365 111 + 3.179 3.112 -0.222
ENSG00000132780 E021 1.1138898 1.637214e-02 6.192624e-01   1 45604917 45604935 19 + 0.281 0.375 0.594
ENSG00000132780 E022 971.9058256 4.958851e-04 2.160509e-06 5.587090e-05 1 45604936 45604965 30 + 3.016 2.954 -0.208
ENSG00000132780 E023 1248.0467165 8.995142e-05 7.189795e-13 7.550974e-11 1 45604966 45605016 51 + 3.127 3.061 -0.219
ENSG00000132780 E024 1165.9653265 9.589934e-05 5.966015e-09 2.934163e-07 1 45606482 45606516 35 + 3.092 3.039 -0.178
ENSG00000132780 E025 1400.8918954 1.629661e-04 3.684556e-06 8.850386e-05 1 45606517 45606591 75 + 3.166 3.126 -0.133
ENSG00000132780 E026 56.0728898 1.137300e-01 2.330954e-02 1.069199e-01 1 45606592 45606680 89 + 1.886 1.545 -1.157
ENSG00000132780 E027 1346.0975942 2.175741e-03 5.213525e-34 5.887465e-31 1 45607321 45607972 652 + 2.954 3.264 1.033
ENSG00000132780 E028 817.5077900 3.990393e-03 4.110648e-20 1.242140e-17 1 45607973 45608337 365 + 2.730 3.052 1.073
ENSG00000132780 E029 280.5345284 1.070446e-01 8.763201e-02 2.593139e-01 1 45609300 45610553 1254 + 2.539 2.321 -0.725
ENSG00000132780 E030 124.7227666 1.222898e-01 2.233438e-01 4.573772e-01 1 45610554 45610995 442 + 2.162 2.016 -0.488
ENSG00000132780 E031 582.7986995 7.756869e-02 4.686361e-01 6.791107e-01 1 45610996 45613168 2173 + 2.792 2.735 -0.187
ENSG00000132780 E032 1378.7113302 1.880807e-04 7.240759e-02 2.295960e-01 1 45613169 45613248 80 + 3.145 3.136 -0.031
ENSG00000132780 E033 1301.9650705 7.647827e-05 5.912462e-01 7.645529e-01 1 45614096 45614181 86 + 3.112 3.117 0.018
ENSG00000132780 E034 1377.2120072 7.478202e-05 7.648755e-01 8.755700e-01 1 45614293 45614366 74 + 3.132 3.146 0.047
ENSG00000132780 E035 5.3631569 5.408031e-03 5.726611e-01 7.521581e-01 1 45614963 45615012 50 + 0.830 0.764 -0.261
ENSG00000132780 E036 1146.8801334 1.432713e-04 8.887986e-02 2.616971e-01 1 45615013 45615058 46 + 3.045 3.076 0.103
ENSG00000132780 E037 1853.8118602 6.200521e-05 6.869373e-01 8.271697e-01 1 45615059 45615201 143 + 3.261 3.276 0.048
ENSG00000132780 E038 2168.0790541 3.592187e-04 5.025900e-02 1.802992e-01 1 45615305 45615471 167 + 3.320 3.353 0.109
ENSG00000132780 E039 17.7216409 4.288862e-02 6.881239e-01 8.279255e-01 1 45615472 45615715 244 + 1.287 1.241 -0.162
ENSG00000132780 E040 1211.6446467 1.091034e-03 1.820722e-01 4.066823e-01 1 45616337 45616393 57 + 3.066 3.101 0.117
ENSG00000132780 E041 1488.2056599 6.962376e-04 1.650592e-02 8.365920e-02 1 45616626 45616700 75 + 3.149 3.196 0.156
ENSG00000132780 E042 993.3003889 4.805980e-04 3.730563e-01 6.065500e-01 1 45616701 45616703 3 + 2.985 3.008 0.078
ENSG00000132780 E043 24.8268114 4.989620e-02 6.187600e-01 7.830080e-01 1 45617237 45617462 226 + 1.430 1.383 -0.162
ENSG00000132780 E044 1006.2288961 1.285146e-03 4.833163e-02 1.757639e-01 1 45617463 45617464 2 + 2.978 3.028 0.165
ENSG00000132780 E045 1806.0908791 1.154760e-04 3.664755e-08 1.510948e-06 1 45617465 45617591 127 + 3.225 3.288 0.208
ENSG00000132780 E046 1210.9229003 9.240972e-05 4.472932e-12 4.114879e-10 1 45618061 45618904 844 + 3.040 3.127 0.287