ENSG00000132475

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254810 ENSG00000132475 HEK293_OSMI2_6hA HEK293_TMG_6hB H3-3B protein_coding protein_coding 618.266 437.2808 565.5797 42.91134 26.16058 0.3711628 110.5777 53.96865 106.3005 5.864188 5.085216 0.977823498 0.1743375 0.1233000 0.1880000 0.06470000 2.210181e-03 9.201542e-30 FALSE TRUE
ENST00000589599 ENSG00000132475 HEK293_OSMI2_6hA HEK293_TMG_6hB H3-3B protein_coding protein_coding 618.266 437.2808 565.5797 42.91134 26.16058 0.3711628 209.7683 170.31937 169.8768 17.284877 9.648321 -0.003753846 0.3415375 0.3892333 0.3002667 -0.08896667 1.004037e-03 9.201542e-30 FALSE TRUE
ENST00000592643 ENSG00000132475 HEK293_OSMI2_6hA HEK293_TMG_6hB H3-3B protein_coding protein_coding 618.266 437.2808 565.5797 42.91134 26.16058 0.3711628 148.9396 110.57004 121.2662 6.664706 9.798833 0.133205807 0.2413125 0.2546667 0.2137667 -0.04090000 4.236805e-01 9.201542e-30 FALSE FALSE
ENST00000593254 ENSG00000132475 HEK293_OSMI2_6hA HEK293_TMG_6hB H3-3B protein_coding processed_transcript 618.266 437.2808 565.5797 42.91134 26.16058 0.3711628 135.2352 75.01962 162.4461 8.452627 5.719772 1.114517702 0.2181583 0.1712333 0.2877667 0.11653333 1.263970e-06 9.201542e-30 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000132475 E001 2068.886500 2.076624e-03 9.946838e-09 4.688321e-07 17 75776434 75778019 1586 - 3.381 3.253 -0.425
ENSG00000132475 E002 480.479803 1.379787e-03 1.039370e-05 2.168681e-04 17 75778020 75778071 52 - 2.744 2.633 -0.370
ENSG00000132475 E003 1374.600627 2.557452e-04 1.200651e-46 3.185909e-43 17 75778072 75778249 178 - 3.233 3.053 -0.598
ENSG00000132475 E004 1715.197992 1.026617e-04 8.019071e-32 7.441654e-29 17 75778250 75778324 75 - 3.300 3.182 -0.393
ENSG00000132475 E005 2325.958878 1.467569e-04 7.818706e-12 6.844310e-10 17 75778325 75778452 128 - 3.404 3.339 -0.217
ENSG00000132475 E006 1870.914225 3.423398e-04 3.582451e-01 5.944321e-01 17 75778453 75778566 114 - 3.278 3.269 -0.030
ENSG00000132475 E007 609.890675 1.753960e-03 1.721373e-02 8.623096e-02 17 75778567 75778573 7 - 2.745 2.816 0.236
ENSG00000132475 E008 612.540773 1.788752e-03 3.292733e-01 5.682837e-01 17 75778574 75778575 2 - 2.769 2.802 0.112
ENSG00000132475 E009 1350.457834 5.571385e-04 9.559092e-02 2.740787e-01 17 75778576 75778609 34 - 3.115 3.144 0.097
ENSG00000132475 E010 1446.858100 6.783334e-05 4.245323e-02 1.611321e-01 17 75778610 75778637 28 - 3.156 3.169 0.044
ENSG00000132475 E011 1362.461866 9.074033e-05 1.862919e-01 4.121610e-01 17 75778638 75778646 9 - 3.133 3.141 0.025
ENSG00000132475 E012 1995.905904 5.948233e-05 3.661272e-06 8.800682e-05 17 75778647 75778723 77 - 3.284 3.317 0.110
ENSG00000132475 E013 17.326642 4.066138e-02 2.604656e-01 4.994191e-01 17 75778724 75778809 86 - 1.339 1.198 -0.495
ENSG00000132475 E014 1236.219780 1.368971e-04 8.682518e-05 1.328012e-03 17 75778810 75778820 11 - 3.072 3.112 0.133
ENSG00000132475 E015 1749.528502 7.478867e-05 1.717478e-08 7.647280e-07 17 75778821 75778882 62 - 3.219 3.265 0.153
ENSG00000132475 E016 1769.068608 5.820156e-04 1.011493e-12 1.036661e-10 17 75778883 75778963 81 - 3.184 3.295 0.370
ENSG00000132475 E017 20.694083 6.596797e-03 3.321451e-01 5.710001e-01 17 75778964 75779046 83 - 1.286 1.366 0.278
ENSG00000132475 E018 2115.189628 3.982865e-04 4.826549e-19 1.234426e-16 17 75779047 75779184 138 - 3.259 3.375 0.383
ENSG00000132475 E019 14.156979 9.767455e-02 8.017846e-01 8.973797e-01 17 75779185 75779541 357 - 1.116 1.230 0.404
ENSG00000132475 E020 7.600220 2.165561e-01 1.000000e+00 1.000000e+00 17 75779542 75779646 105 - 0.912 0.956 0.165
ENSG00000132475 E021 7.967167 5.665633e-03 4.160840e-02 1.588986e-01 17 75779647 75779656 10 - 0.799 1.042 0.920
ENSG00000132475 E022 823.161892 9.457408e-04 2.826704e-03 2.235236e-02 17 75779657 75779661 5 - 2.881 2.945 0.212
ENSG00000132475 E023 1368.267036 1.211544e-03 1.343080e-06 3.672677e-05 17 75779662 75779780 119 - 3.075 3.182 0.354
ENSG00000132475 E024 2.865102 2.313024e-01 4.734186e-01 6.824093e-01 17 75783107 75784520 1414 - 0.380 0.694 1.496
ENSG00000132475 E025 1.896594 2.098982e+00 4.394992e-01 6.582890e-01 17 75784521 75785375 855 - 0.589 0.322 -1.389
ENSG00000132475 E026 4.364097 3.383113e+00 7.517339e-01 8.678153e-01 17 75785376 75785893 518 - 0.916 0.478 -1.852