ENSG00000132300

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254630 ENSG00000132300 HEK293_OSMI2_6hA HEK293_TMG_6hB PTCD3 protein_coding protein_coding 44.19304 13.68175 84.16769 1.528124 2.968904 2.620131 8.498169 3.3828429 13.861136 0.54622986 0.1885360 2.031519 0.21517917 0.24566667 0.16513333 -0.08053333 0.2359982202 0.0001074943 FALSE TRUE
ENST00000464541 ENSG00000132300 HEK293_OSMI2_6hA HEK293_TMG_6hB PTCD3 protein_coding retained_intron 44.19304 13.68175 84.16769 1.528124 2.968904 2.620131 2.305998 0.5734843 5.173293 0.07669696 0.5082106 3.151103 0.05113333 0.04176667 0.06196667 0.02020000 0.5541878322 0.0001074943   FALSE
ENST00000467273 ENSG00000132300 HEK293_OSMI2_6hA HEK293_TMG_6hB PTCD3 protein_coding retained_intron 44.19304 13.68175 84.16769 1.528124 2.968904 2.620131 2.513154 0.5281082 5.382723 0.05499045 0.4335823 3.325046 0.05161667 0.03883333 0.06380000 0.02496667 0.2185519759 0.0001074943 FALSE FALSE
ENST00000476215 ENSG00000132300 HEK293_OSMI2_6hA HEK293_TMG_6hB PTCD3 protein_coding retained_intron 44.19304 13.68175 84.16769 1.528124 2.968904 2.620131 2.624434 0.7284584 5.792458 0.23771446 0.8667462 2.974076 0.05572917 0.05793333 0.06830000 0.01036667 0.8703083860 0.0001074943   FALSE
ENST00000484203 ENSG00000132300 HEK293_OSMI2_6hA HEK293_TMG_6hB PTCD3 protein_coding retained_intron 44.19304 13.68175 84.16769 1.528124 2.968904 2.620131 7.614652 1.8646224 16.407286 0.14728468 1.0277152 3.130544 0.14752917 0.13850000 0.19470000 0.05620000 0.3222757622 0.0001074943 FALSE TRUE
ENST00000487043 ENSG00000132300 HEK293_OSMI2_6hA HEK293_TMG_6hB PTCD3 protein_coding retained_intron 44.19304 13.68175 84.16769 1.528124 2.968904 2.620131 3.229550 1.5992902 4.000009 0.09277251 0.2409430 1.317181 0.10721667 0.11850000 0.04753333 -0.07096667 0.0001074943 0.0001074943 FALSE TRUE
MSTRG.18785.6 ENSG00000132300 HEK293_OSMI2_6hA HEK293_TMG_6hB PTCD3 protein_coding   44.19304 13.68175 84.16769 1.528124 2.968904 2.620131 9.610847 2.8798972 19.357735 0.90556232 1.2785654 2.744565 0.20375000 0.20143333 0.23016667 0.02873333 0.8905341051 0.0001074943 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000132300 E001 1.2734679 0.0306650654 7.049698e-01   2 86106223 86106223 1 + 0.288 0.367 0.494
ENSG00000132300 E002 1.2734679 0.0306650654 7.049698e-01   2 86106224 86106225 2 + 0.288 0.367 0.494
ENSG00000132300 E003 1.2734679 0.0306650654 7.049698e-01   2 86106226 86106233 8 + 0.288 0.367 0.494
ENSG00000132300 E004 1.4161025 0.0677525386 8.232160e-01 0.909805219 2 86106234 86106234 1 + 0.321 0.367 0.280
ENSG00000132300 E005 1.8981367 0.0168385008 8.062930e-01 0.899927104 2 86106235 86106235 1 + 0.410 0.367 -0.244
ENSG00000132300 E006 16.6297573 0.0024133998 9.546932e-01 0.979253425 2 86106236 86106243 8 + 1.148 1.153 0.020
ENSG00000132300 E007 17.7788240 0.0041927382 8.846348e-01 0.943732193 2 86106244 86106247 4 + 1.177 1.173 -0.013
ENSG00000132300 E008 83.9922051 0.0005090702 6.410094e-01 0.797513078 2 86106248 86106351 104 + 1.825 1.860 0.117
ENSG00000132300 E009 0.3040503 0.0266630524 1.000000e+00   2 86107118 86107200 83 + 0.119 0.000 -9.402
ENSG00000132300 E010 0.0000000       2 86108345 86108349 5 +      
ENSG00000132300 E011 99.2841751 0.0004785121 3.446955e-01 0.582222443 2 86108350 86108402 53 + 1.892 1.946 0.180
ENSG00000132300 E012 96.5303551 0.0003891668 7.915216e-02 0.243135271 2 86108500 86108536 37 + 1.904 1.836 -0.231
ENSG00000132300 E013 1.0185584 0.0163995028 4.313589e-01   2 86108537 86108925 389 + 0.212 0.367 1.077
ENSG00000132300 E014 0.5117739 0.0230030934 5.519267e-01   2 86110978 86111112 135 + 0.119 0.221 1.074
ENSG00000132300 E015 111.4348724 0.0004215538 1.053908e-01 0.291331043 2 86111113 86111158 46 + 1.963 1.905 -0.194
ENSG00000132300 E016 0.0000000       2 86111790 86112095 306 +      
ENSG00000132300 E017 136.8790799 0.0003760197 9.887459e-02 0.279820342 2 86116530 86116598 69 + 2.052 2.001 -0.172
ENSG00000132300 E018 186.7448610 0.0006040358 2.119150e-01 0.443984337 2 86117055 86117159 105 + 2.181 2.152 -0.099
ENSG00000132300 E019 200.7392266 0.0004031490 2.953163e-01 0.535795266 2 86118921 86119044 124 + 2.213 2.192 -0.069
ENSG00000132300 E020 0.5008152 0.0400306514 6.547362e-01   2 86119045 86119181 137 + 0.168 0.000 -10.000
ENSG00000132300 E021 9.8719742 0.0078639581 8.442823e-01 0.921728433 2 86119544 86119614 71 + 0.962 0.950 -0.045
ENSG00000132300 E022 0.5008152 0.0400306514 6.547362e-01   2 86121465 86121478 14 + 0.168 0.000 -10.000
ENSG00000132300 E023 112.7697896 0.0022453536 1.070781e-01 0.294111601 2 86121479 86121493 15 + 1.973 1.910 -0.212
ENSG00000132300 E024 205.4076754 0.0002606617 4.738052e-02 0.173459989 2 86121494 86121594 101 + 2.228 2.178 -0.167
ENSG00000132300 E025 194.8927635 0.0002308454 9.184748e-03 0.054640983 2 86123701 86123762 62 + 2.206 2.134 -0.244
ENSG00000132300 E026 215.7712488 0.0002646270 5.801613e-03 0.038751123 2 86124995 86125082 88 + 2.250 2.176 -0.247
ENSG00000132300 E027 181.0223308 0.0002738792 1.339905e-02 0.072023686 2 86125455 86125515 61 + 2.174 2.103 -0.239
ENSG00000132300 E028 160.5787087 0.0022818598 7.387728e-01 0.859682925 2 86125795 86125880 86 + 2.111 2.110 -0.002
ENSG00000132300 E029 205.9255704 0.0003344584 3.767752e-01 0.609672151 2 86127161 86127305 145 + 2.211 2.251 0.134
ENSG00000132300 E030 61.2744055 0.0027485277 1.093019e-03 0.010622020 2 86127306 86127661 356 + 1.726 1.516 -0.715
ENSG00000132300 E031 21.0334646 0.0114572179 6.558361e-01 0.807219098 2 86127719 86127871 153 + 1.252 1.211 -0.145
ENSG00000132300 E032 15.6847508 0.0020820366 1.729226e-01 0.394373505 2 86127872 86127929 58 + 1.153 1.011 -0.511
ENSG00000132300 E033 22.0648364 0.0015574432 6.545018e-01 0.806389146 2 86127930 86127940 11 + 1.273 1.244 -0.103
ENSG00000132300 E034 122.7742109 0.0007177279 4.393783e-01 0.658203717 2 86127941 86127948 8 + 1.986 2.031 0.150
ENSG00000132300 E035 146.0938099 0.0003488896 1.790378e-01 0.402704493 2 86127949 86127991 43 + 2.058 2.118 0.204
ENSG00000132300 E036 109.4351515 0.0003955466 4.223635e-01 0.645414994 2 86130648 86130650 3 + 1.935 1.981 0.154
ENSG00000132300 E037 184.6189047 0.0003387336 8.647971e-01 0.933028623 2 86130651 86130737 87 + 2.169 2.176 0.022
ENSG00000132300 E038 23.2150595 0.0014472961 1.259879e-03 0.011862004 2 86130738 86130889 152 + 1.334 1.012 -1.151
ENSG00000132300 E039 32.1248792 0.0031150756 9.664612e-05 0.001452840 2 86130890 86131077 188 + 1.470 1.111 -1.257
ENSG00000132300 E040 133.0745962 0.0053637728 9.389714e-01 0.971794446 2 86131078 86131106 29 + 2.027 2.036 0.029
ENSG00000132300 E041 232.1786503 0.0002907023 9.006775e-01 0.952212280 2 86132318 86132424 107 + 2.268 2.276 0.028
ENSG00000132300 E042 39.2957481 0.1060453124 1.759705e-01 0.398424608 2 86132425 86132823 399 + 1.542 1.315 -0.784
ENSG00000132300 E043 15.8468787 0.1141883328 4.266342e-01 0.648506805 2 86132824 86132877 54 + 1.154 0.997 -0.567
ENSG00000132300 E044 22.9432532 0.0036931882 3.120681e-02 0.130942468 2 86132878 86133063 186 + 1.318 1.111 -0.733
ENSG00000132300 E045 17.2325328 0.0129666324 1.482391e-02 0.077409617 2 86133064 86133177 114 + 1.215 0.917 -1.087
ENSG00000132300 E046 178.5850717 0.0003618375 9.940167e-01 0.998620288 2 86133178 86133256 79 + 2.156 2.168 0.041
ENSG00000132300 E047 11.3110598 0.0029158824 2.782066e-02 0.120774742 2 86133257 86133268 12 + 1.044 0.751 -1.118
ENSG00000132300 E048 16.8228066 0.0021588159 4.850840e-02 0.176206752 2 86133269 86133322 54 + 1.193 0.983 -0.764
ENSG00000132300 E049 13.4632372 0.0130921588 1.387287e-01 0.345581518 2 86133323 86133345 23 + 1.105 0.918 -0.689
ENSG00000132300 E050 199.2737224 0.0023261754 6.904640e-01 0.829366508 2 86133346 86133436 91 + 2.200 2.227 0.091
ENSG00000132300 E051 86.1939544 0.0016427879 6.742885e-01 0.819076703 2 86134292 86134295 4 + 1.839 1.874 0.118
ENSG00000132300 E052 186.6919882 0.0002492960 7.626089e-01 0.874146181 2 86134296 86134377 82 + 2.172 2.194 0.075
ENSG00000132300 E053 263.2156444 0.0001987463 9.960796e-03 0.057998089 2 86134839 86134987 149 + 2.308 2.388 0.267
ENSG00000132300 E054 11.8344916 0.0055724423 6.214842e-01 0.784852912 2 86134988 86135198 211 + 0.998 1.065 0.243
ENSG00000132300 E055 156.8899416 0.0004906664 7.073431e-02 0.226207800 2 86136521 86136562 42 + 2.085 2.160 0.250
ENSG00000132300 E056 18.4264303 0.0305942879 8.167341e-03 0.050101225 2 86136563 86136881 319 + 1.110 1.420 1.090
ENSG00000132300 E057 282.8714811 0.0022984103 1.198427e-01 0.315600758 2 86136982 86137140 159 + 2.342 2.404 0.208
ENSG00000132300 E058 801.2541772 0.0039524255 3.446565e-04 0.004184942 2 86137469 86142158 4690 + 2.780 2.889 0.361